Pinus roxburghii (P. roxburghii) is an important ecological and industrial conifer of the Himalayas. Plant boom is incredibly impacted by way of environmental stresses, in particular high ranges of nitrogen (N) and ozone (O3). This work aims to explore how these factors affect the total protein content and chlorophyll levels of P. roxburghii. Three consortiums were inoculated with two-year-old P. roxburghii seedlings. The stresses of 100 kg N h−1 and 100 ppb O3 were applied for 1 month to study their effect on chlorophyll level and total protein content. To evaluate their potential mitigating effects, the fungal consortium presented promising outcomes for the chosen plant species. The elevated metabolic activities and photosynthesis rate were determined by improved total protein content and high chlorophyll level (p