Improved Methods for Encoding and Decoding Dialkylamine-Encoded Combinatorial Libraries

WL Fitch, TA Baer, WW Chen,F Holden,CP Holmes,D Maclean, NH Shah,E Sullivan, M Tang, P Waybourn,SM Fischer,CA Miller, LR Synder

Journal of combinatorial chemistry（1999）

Affymax Res Inst

被引用49|浏览36

摘要

Optimized methods for utilizing and analyzing dialkylamine tags in encoded combinatorial chemistry are reported. A previously described LC/fluorescence method has been shortened from over 1 h to 6 min. A novel ion exchange LC/MS method for decoding has been developed which is both fast and rugged. The use of quantitative encoding schemes is described. These new techniques have allowed a simplified, automated sample preparation scheme, and over 300 encoded bead samples can be read in a day.

更多

查看译文