Papilionanthe teres (Roxb.) Schltr. is a medicinal orchid found in all reserve forests of Southern Assam, India. Mature pods of Papilionanthe teres were collected from natural plants and seeds were inoculated in Murashige and Skoog (MS), Knudson C (KnC), Commercial Orchid Maintenance(OMM) and Commercial Orchid Maintenance Replate (OMR) medium(HiMedia). Best germination and growth were observed in commercial orchid maintenance replate medium supplemented with 2mg\L IAA and 5mg\L KN. Growth of root, shoot were measured after three months of germination. Shoot tip and nodal explant of the plant were tried in different medium viz. MS, White, B5, KnC, Commercial Orchid Maintenance medium etc. containing different growth regulators modification for micro propagation. For phytochemical screening qualitative tests were performed from the dried powder of the plants. From the qualitative test it was observed that the plant extracts contain alkaloid, flavonoid and tannin in petroleum ether extract and alkaloid, tannin and saponin were found in ethyl acetate extract. Steroid was found present in methanol and benzene extract. Flavonoid was found only in acetone extract.
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