Chelerythrine is one of the most abundant alkaloids in the traditional Chinese medicinal herb Chelidonium majus L. We aimed to develop a wettable powder formulation containing chelerythrine,and to clarify the mechanism by which chelerythrine induces apoptosis and autophagy in Spodoptera frugiperda clone 9 (Sf9) cells,thereby promoting the application and development of chelerythrine in plant protection.