Inhibitory Effects of the Lactic Acid Bacteria Weissella Koreensis DB1 Cell Extract Derived from Kimchi on the Differentiation in 3T3-L1 Cells

Weissella koreensis (W. koreensis) DB1 with high-ornithine-producing capacity was isolated from kimchi and shows the anti-adipogenic activity via its production of ornithine. This study was carried out the effects of W. koreensis DB1 cell extracts on the inhibition of adipogenesis in 3T3-L1 cells. MTT results showed that 0-0.75 μg/mL W. koreensis DB1 cell extracts had no significant effect on cell vability. The cell extracts of W. koreensis DB1 at non-cytotoxic concentrations significantly suppressed the differentiated 3T3-L1 cells by decreasing triglyceride level and intracellular lipid accumulation compared to that associated with the untreated control group. The major transcriptional factors involved in adipocyte differentiation, such as peroxisome proliferator-activated receptorγ (PPARγ), CCAAT/enhancer-binding proteinα (C/EBPα), and sterol regulatory element-binding transcription factor-1c (SREBP-1c), were down-regulated by the cell extracts of W. koreensis DB1 treatments compared with the untreated control group. Furthermore, the expressions of lipogenesis-related enzymes including acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) genes were also decreased by the cell extracts of W. koreensis DB1 treatment. Accordingly, these results indicate that the cell extracts of W. koreensis DB1 treatment inhibited the adipogenesis and lipogenesis of 3T3-L1 cells, suggesting a protective role in adipocyte differentiation.