Knockout of TaCKX2.2 in Wheat Improved Grain Yield by Increasing Grain Number Per Spike and Grain Size under Field Production Conditions

Summary statement Knockouts of cytokinin oxidase‐dehydrogenase (TaCKX2.2) homeologs increased grain number per spike, grain size, grain weight per spike, and final yield without negative impacts on other major agronomic traits, revealing a novel approach to improve grain yield by manipulating the TaCKX2.2 gene family in wheat.