Large-scale High-Efficiency Yeast Transformation Using the LiAc/SS Carrier DNA/PEG Method

R Daniel Gietz,Robert H Schiestl

Nature Protocols（2007）SCI 1区

Department of Biochemistry and Medical Genetics

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摘要

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae . This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

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Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry