LIFEPLAN Malaise Sample Metabarcoding V1

In Project LIFEPLAN, bulk samples of arthropods are collected with Malaise traps and the species are identified with a non-destructive COI metabarcoding process. This protocol describes the steps for lysis, DNA extraction, PCR amplification, library construction, sequencing and sequence analysis, going from bulk arthropod samples to COI sequences, aiming at 1 million reads per bulk sample.