Mapping Regulatory Elements Using Single-cell ATAC-Seq.

The Medical Genome Facility Genome Analysis Core has successfully performed single-cell transcriptome experiments for more than 6 years. Using the Fluidigm C1 and 10x Genomics Chromium platforms, we have captured and labeled single cells from over 470 samples. We continue to expand the number of procedures we perform for Mayo Clinic investigators, and this year we are making available the 10x Genomics ATAC-Seq (Assay for Transposase-Accessible Chromatin using sequencing) protocol. In collaboration with the Gaspar Maia lab in the Mayo Clinic College of Medicine, we have run pilot experiments charting regulatory elements in an ovarian cancer PDX model. Here we discuss our methods and present some promising preliminary results.