We present a new measurement of the branching ratio of the Cabibbo suppressed decay D0 → π−μ+ν relative to the Cabibbo favored decayD0 → K−μ+ν and an improved measurement of the ratio |f π + (0)/f K + (0)|. Our results are 0 . 74±0.008±0.007 for the branching ratio and 0 .85± 0.04± 0.04± 0.01 for the form factor ratio, respectively. 2004 Elsevier B.V.