The spectacled cormorant, Pallas cormorant (Urile perspicillatus (Suliformes: Phalacrocoracidae)), played an important role as a source of animal protein for sailors, fur traders, and the permanent population of the Commander Islands, Russia. This endemic bird species was hunted to extinction due to human activity in the mid-19th century. Currently, there is no published genetic information available about the spectacled cormorant, and its phylogenetic relationships are primarily based on morphological traits. This study aims to address this knowledge gap by presenting two complete mitochondrial genomes from two historical specimens of this extinct species, dating back to 1510 − 1220 cal BP and 500 − 230 cal BP years, respectively. We sequenced the ancient DNA of the extinct spectacled cormorant from Bering Island in order to reconstruct its complete mitochondrial genome and determine its phylogenetic position among other extant species in the Phalacrocoracidae family. The mtDNA of the spectacled cormorant was assembled and annotated, revealing that its mitogenome is 16,895 base pairs long. It contains 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes, with an overall base composition of 32.3
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