MS16 Time- Resolved Diffraction and Scattering Techniques MS16-1-2

Serial crystallography (SX) at both X-ray free-electron lasers (XFELs) and synchrotrons offers the possibility to collect data at non-cryogenic temperature almost radiation damage free and enables time-resolved crystallography of irreversible reactions. The necessary steady delivery of new micron-sized crystals of biological macromolecules sets it apart from traditional single crystal macromolecular crystallography (MX). For this, many new means of sample delivery have been developed [1]. Described here is a novel conveyor belt-based sample delivery system, the completely re-designed and re-engineered second generation of the CFEL TapeDrive. It is optimized for fast installation at beamlines, ease of use, low sample consumption and precise adjustment of several sample delivery parameters, like ligand concentration, pH and sample temperature. Through combination of these parameters and the additional possibility for time-resolved experiments (delay times of 50 ms – 180 s), CFEL TapeDrive 2.0 enables multidimensional serial crystallography experiments. It can now be used as standard instrumentation at beamline P11 at PETRA III (DESY, Germany) and is suitable for serial Laue crystallography with polychromatic X-rays. Additionally, it can be used at XFELs in air (e.g. at SwissFEL