MULTI-Seq Barcoding and Library Preparation V1

This protocol describes MULTI-Seq barcoding of hESCs and library preparation, it is based on McGinnis et. al. 2019. PMID: 31209384 and the 10x Genomics user guide “Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 with Feature Barcoding technology for CRISPR Screening” Protocol overview A. Oligonucleotides B. Sequencing Initial notes A list of reagents and relevant vendor information can be found in the table listed under the materials tab.