Triclosan (TCS), triclocarban (TCC), and chlorophene (CP) are widely used antimicrobial agents with persistent environmental residues, posing potential ecological and health risks. Thus, developing a sensitive, rapid on-site screening method is highly significant. In this study, computer simulation was first employed to screen the haptens of TCS, TCC and CP to prepare highly specific and high affinity monoclonal antibodies with half-maximal inhibitory concentrations of 1.044, 0.964, and 1.704 ng/mL, respectively. Based on this, a multi-immunochromatography assay (MICA) was established to simultaneously and rapidly detect TCS, TCC, and CP in lake water, wastewater and sludge, with calculated limits of detection of 1.138, 1.090, and 2.525 μg/kg in lake water; 1.147, 1.093, and 2.511 μg/kg in wastewater; and 1.668, 1.493, and 3.106 μg/kg in sludge, respectively. Analysis of real samples confirmed strong consistency between MICA and HPLC-MS/MS results, with high accuracy, stability, and reliability, supporting its application for on-site screening of large batches of samples.
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