Neuroprotective Effect of the Silver Nanoparticles Green Formulated by Pinellia Ternata Extract on Cerebral Ischemia-reperfusion Injury in Rats

Cerebral ischemia treatment remains a clinical problem. According to studies, cerebral ischemia is primarily caused by the neurovascular unit, which serves as the main structural foundation. The new silver nanoparticles (AgNPs) green produced by Pinellia ternata are used in this work to treat cerebral ischemic stroke-reperfusion damage in rats. Several physicochemical methods, including ultraviolet–visible spectroscopy (UV-Vis), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX), were used to analyze the post-synthetically altered biogenic material in detail. Following transient middle cerebral artery occlusion and reperfusion, rats received oral administration of AgNPs@Pinellia ternata (10, 30, 90–270 µg/kg) once daily for 14 consecutive days. Behavioral, biochemical and histological assessments were subsequently performed to evaluate neuroprotective efficacy. The index of cell damage was measured by investigating infarct volume, learning and memory function, lactate dehydrogenase activity and serum malondialdehyde (MDA) level, and extravasation of cerebral parenchyma immunoglobulin G (IgG); treatment with AgNPs@Pinellia ternata significantly decreased the lactate dehydrogenase activity, infarct volume and serum MDA level, and IgG extravasation was significantly inhibited. Ischemic stroke-reperfusion injury-induced learning and memory impairments may be prevented by treatment with AgNPs@Pinellia ternata. It was demonstrated that treatment with AgNPs@Pinellia ternata protected rats against cerebral ischemic stroke-reperfusion injury.