To investigate the chemical constituents of Tripterygium wilfordii Hook. f. and their chemotaxonomic significance, twelve compounds were isolated and identified from this plant, including nine sesquiterpenoids (1-6, 8, 11-12), two monoterpenoids (9-10), and one lignan (7). The occurrence of compounds 1, 3-12 in Tripterygium has been reported for the first time. By analyzing the distribution patterns of these compounds across different plant taxa, the chemotaxonomic significance of the isolated compounds was systematically elucidated.