Preparation of PCR-quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (Hotshot).

BioTechniquesVol. 29, No. 1 BenchmarksOpen AccessPreparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT)G.E. Truett, P. Heeger, R.L. Mynatt, A.A. Truett, J.A. Walker & M.L. WarmanG.E. Truett*Address correspondence to Dr. Gary E. Truett, Developmental Genetics Laboratory, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808, USA. e-mail: E-mail Address: truettge@pbrc.eduPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this author, P. HeegerPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this author, R.L. MynattPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this author, A.A. TruettPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this author, J.A. WalkerPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this author & M.L. WarmanPennington Biomedical Research Center, Baton Rouge, LA, USASearch for more papers by this authorPublished Online:29 Aug 2018https://doi.org/10.2144/00291bm09AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit "Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT)." , 29(1), pp. 52–54FiguresReferencesRelatedDetailsCited ByLrig1 expression identifies quiescent stem cells in the ventricular-subventricular zone from postnatal development to adulthood and limits their persistent hyperproliferation11 January 2023 | Neural Development, Vol. 18, No. 1Reduction in mitochondrial ROS improves oxidative phosphorylation and provides resilience to coronary endothelium in non-reperfused myocardial infarction13 January 2023 | Basic Research in Cardiology, Vol. 118, No. 1Biogeography, climate, and land use create a mosaic of parasite risk in native bumble beesScience of The Total Environment, 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