Processing Single Cell Calling Card Sequencing Data V1

Here we present a computational pipeline for processing single cell calling card (scCC) data. These data will have been generated from single cell RNA-seq libraries following transfection/-duction of either undirected piggyBac transposase or your favorite transcription factor (YFTF) fused to piggyBac. This workflow demonstrates how to process scCC sequencing data derived from a 10x Chromium-based scCC library; the workflow can be parallelized on distributed computing architectures (e.g. slurm).