In areas such as compuer vision and image processing,image thresholding has been and still is a relevant research area due to its wide spread usage and application.This paper try to undertake on image thresholding techniques on human DNA. The DNA image is used to identify the genetic disorderness. The microeletronic scope captured the uncleared image and it is too difficult to understand. This work try to analysis the content of DNA image. Medical image processing techniques are apply on DNA image. Variuos preprocessing techniques are performed like median filter, wiener filter, contrast stretching and riddler’s modified thresholding algorithm for thresholding the DNA image. In this paper proposed riddler thresholding modified algorithm to find the solution for median value using metrics. In future, the work done by this paper helps to do the segmentation on human DNA images