БИО- И ГЕМОСОВМЕСТИМОСТЬ ТКАНЕИНЖЕНЕРНЫХ КОНСТРУКЦИЙ ИЗ ПОЛИКАПРОЛАКТОНА, ЗАСЕЛЕННЫХ ЧЕЛОВЕЧЕСКИМИ ЭНДОТЕЛИАЛЬНЫМИ И ГЛАДКОМЫШЕЧНЫМИ КЛЕТКАМИ, ПОСЛЕ ИМПЛАНТАЦИИ В БРЮШНУЮ АОРТУ МЫШАМ SCID

Introduction . The modern development of chemistry and chemical technologies allow us to produce synthetic materials that exhibit physical characteristics similar to the characteristics of the tissues of the body, a satisfactory biocompatible and thromboresistant. Such materials are demanded in vascular surgery, but their practical use is required to improve their biological properties, namely the ability to form normal endothelium on the inner surface and the cell layers inside the wall. Biodegradable synthetic polymeric 3D matrices, populated by functional endothelial (EC) and smooth muscle (SMC) cells of the recipient can be a suitable option for reconstructive surgical interventions on the vessels. The purpose . To evaluate bio - and hemocompatible of the tissue-engineered polycaprolactone scaffolds, seeded by donors EC and SMC in experiment in vivo. Materials and methods . 3D-matrices (polycaprolactone+ gelatin) made by electrospinning method, seeded in vitro human EC and SMC, which were obtained from heart tissue. Cells were cultured on these matrices for 7 days, then were implanted in the abdominal aorta of 23 SCID mice. 3D matrices without cells were implanted 12 mice for control. After 2, 4, 12 and 24 weeks after implantation, performed ultrasound scan and MRI of the abdominal aorta, immune histological studies explanted patches. Results . Polycaprolactone patchs seeded by functional EC and SMC enhanced local neovascularization in the area of their implantation, with the development of a vascularized fibrous capsule, promote adhesion and proliferation of EC and SMC, provide good patency of the mice abdominal aorta during 24 weeks of observation. Conclusion. Cell-populated polycaprolactone matrices can be used to create patient-specific vascular grafts.