Selection Analysis of Single-Stranded Clones Using Solid-Phase Techniques.

T Vener,A Holmberg,J Lundeberg,M Uhlén

Genetic analysis biomolecular engineering（1999）

KTH

被引用2|浏览1

摘要

An efficient selection analysis of single-stranded DNA fragments using subtractive hybridization on magnetic beads and fluorescence-based fragment analysis is described. This approach facilitates either direct cloning of captured fragments or removal of common clones from cDNA or shot-gun clone libraries.

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关键词

magnetic separation,solid phase,cloning,library,hybridization,genome sequencing

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