ShinyMultiome.UiO: an Interactive Open-Source Framework Utilizing Seurat Objects for Visualizing Single-Cell Multiomes

Motivation Single-cell genomics has been revolutionized by the advent of single-cell Multiome sequencing which allows for simultaneous profiling of chromatin accessibility and gene expression in individual nuclei. The analysis and interpretation of large and often complex scMultiome datasets requires in-depth knowledge of computational programming. Results We present ShinyMultiome.UiO, a user-friendly, integrative, and opensource web-based tool that supports Seurat objects for visualization of single-cell Multiome. The ShinyMultiome.UiO facilitates interactive reporting and comprehensive characterization of cellular heterogeneity and regulatory landscapes. With ShinyMultiome.UiO, users can facilitate collaborative efforts for interpre-tation of single-cell Multiome data and make it available to the public. Availability and implementation https://github.com/EskelandLab/ShinyMultiomeUiO and demo server, https://cancell.medisin.uio.no/ShinyMultiome.UiO/ set up with a PBMC reference dataset. Contact Ankush.Sharma@medisin.uio.no & Ragnhild.Eskeland@medisin.uio.no