MALDI-TOF MS analysis of supercritical CO 2 extracted samples obtained from Mandevilla velutinacell cultures allowed the detection of the anti-bradykinin pregnanic steroid, velutinol A, using low amount of sample (1 g lyophilized cells), with minimum analyte isolation.
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MALDI-TOF MS,Mandevilla velutina,secondary metabolites,supercritical fluid extraction,velutinol A