Supplemental Figure S4 from Therapeutically Active RIG-I Agonist Induces Immunogenic Tumor Cell Killing in Breast Cancers

Immunohistochemistry was used to measure Ki67 and TUNEL+ cells in tumors harvested at day 5. Representative Images are shown. N = 5. Immunohistochemistry was used to measure Ki67 and TUNEL+ cells in tumors harvested at day 5. Representative Images are shown. N = 5. Western analysis of whole cell lysates collected 16 hours after transfection using antibodies indicated at the left of each panel. MCF7 Cells were transfected, and after 16 h, total RNA was assessed by RT-qPCR to measure expression of the indicated genes involved in pyroptosis. Each point represents the average of three experimental replicates, N = 3. Midlines are average {plus minus} S.D. Student's T-test.