Supplementary Figure 10 from Ran is a Potential Therapeutic Target for Cancer Cells with Molecular Changes Associated with Activation of the PI3K/Akt/mTORC1 and Ras/MEK/ERK Pathways

PDF file - 1.5MB, The importance of nucleocytoplasmic transportation and Mcl-1 expression in Ran silencing-induced apoptosis in A549 cells. Cells were grown in different conditions at 24 hours post-infection and were then harvested at 72 hours post-infection. Percentage of sub-G1 phase apoptotic cells are shown in A549 cells treated with (A) rapamycin and/or LMB, and (B) PI103 and/or LMB. Western blots of PARP, Mcl-1 and Ran, in A549 cells expressing various levels of Ran treated with (C) rapamycin and/or LMB, and (D) PI103 and/or LMB. Western blots of various transcription factors in cytoplasmic and nuclear fraction of scramble (Scr) and Ran knockdown (E) A549 cells, (F) A549 cells with or without rapamycin, (G) A549 cells with or without PD184352. (H) Quantitative PCR of Mcl-1 transcript in A549 cells infected with Scr and Ran shRNAs. (I) Western blots of Mcl-1, PARP and Ran at 72hr post-infection of Ran shRNAs in A549 cells grown in Hank's buffered salt solution (HBSS), serum-free and serum-supplemented conditions. (J) Quantification of Mcl-1 protein levels from figure (I). (K) Western blots of PARP, Mcl-1 and Bcl-2 in MDA MB231 cells upon Ran silencing in the presence of different inhibitors. (L) Quantification of Mcl-1 protein levels from figure.