Supplementary Figure 2 from Spatial and Single-Cell Analyses Reveal Heterogeneity of DNAM-1 Receptor–Ligand Interactions That Instructs Intratumoral Γδt-Cell Activity

Supplementary Figure 2 Gating strategies of FACS analysis and the expression pattern of TIGIT in NB. (A) Gating strategies for the FACS analysis of circulating γδT cells. (B) Gating strategies for the FACS analysis of tumor infiltrating immune cells. (C) and (D) The expression analysis of CD96 and CD112R in peripheral blood (PB) γδT cells from healthy controls (HC, n = 10) and neuroblastoma patients (NB, n = 18). The proportions of γδT cells are shown in the left panel statistically and the representative FACS histograms are shown in the right panel with mean fluorescence intensity (MFI) labeled in corresponding histograms. (E) Using GSE49711 from GEO database and R2: Genomics Analysis and Visualization Platform, the expression pattern of TIGIT in NB tumors (n = 498) with different INSS stages, risks, MYCN status and death events is analyzed and illustrated by box plots. (F) The overall and eventfree survival curves are generated by grouping samples with the median of TIGIT expression. (G) The expression correlation between TIGIT and DNAM-1 are analyzed using GSE49711 dataset. The results are expressed as the means ± SEMs. Significant differences between groups are represented by ns no significance, ** p < 0.01 and **** p < 0.0001.