Supplementary Figures S1-S9 from KIT Suppresses BRAF<sup>V600E</sup>-Mutant Melanoma by Attenuating Oncogenic RAS/MAPK Signaling

This file contains Supplementary Figures S1-S9 with accompanying legends. Supplementary Fig. S1 examines the relationship between KIT expression and BRAFV600E mutations in TCGA human melanoma samples. Supplementary Fig. S2 shows the rescue of melanocytes in kit(lf)-mutant zebrafish by BRAFV600E. Supplementary Fig. S3 shows tumor onset for an additional kit loss-of-function allele as well as tumor invasion images representative of a mosaic analysis. Supplementary Fig. S4 portrays the levels of Mitfa and pAkt in zebrafish melanomas. Supplementary Fig. S5 shows RAF dimerization and signaling experiments performed with V-BRAFV600E stimulated by NRASQ61K. Supplementary Fig. S6 shows RAF dimerization and signaling experiments performed with V-BRAFWT stimulated by KIT and SCF. Supplemental Fig. S7 shows results of experiments overexpressing KIT in A375 and UACC257 human melanoma cells with accompanying growth and signaling changes. Supplementary Fig. S8 shows survival curves for TCGA patients with melanomas expressing high or low KIT. Lastly, Supplementary Fig. S9 displays a measurement of BRAFV600E levels in zebrafish melanomas overexpressing BRAFWT.