Supplementary Figures S1-S9 from Type-I Interferon Signaling is Essential for Robust Metronomic Chemo-Immunogenic Tumor Regression in Murine Breast Cancer

Figures S1-S9 with detailed legends: Fig. S1. Hormone receptor and related gene expression patterns in E0771 and 4T1 tumor cells. (A) Expression in E0771 cells and E0771 tumors, based on RNAseq data included in Table S1 and Table S5; and (B) Expression in 4T1 cells and 4T1 tumors.(C) Summary of hormone receptor expression patterns. Fig. S2. Gene-specific qPCR primer sequences, amplicon length and percent GC content Fig. S3. Dose-dependence of drug sensitivity of cultured 4T1, E0771 and B16F10 cells. Shown are viability assays determined in MTS assays. Fig. S4. ISG induction by 4HC-conditioned culture medium. Fig. S5. Verification of anti-IFNAR-1 antibody inhibitory activity. Fig. S6. E0771 tumor growth curves and qPCR analysis of metronomic-CPA in vivo dose response data. Fig. S7. Representative FACS analysis of blood from CPA-treated mice, with and without anti-CD8a antibody treatment. Fig. S8. Circulating CD8 T-cells for CPA-treated mice with and without anti-CD8a antibody. Fig. S9. FACS analysis of blood and tumors from mice given metronomic CPA treatment with and without anti-IFNAR1 antibody.