Table S3 from Chemoradiotherapy Resistance in Colorectal Cancer Cells is Mediated by Wnt/β-catenin Signaling

Radiation sensitization effect of XAV-939 on CRC cells SW480 and SW837. (A) Axin2 protein levels increase, while active β-catenin and total β-catenin protein levels decreased after incubation with XAV-939 compared to the negative-control (DMSO) in SW480, and SW837 in a dose dependent manner. Proteins were isolated as whole protein lysates (left panel) and cytosolic and nuclear fractions (right panel). (B) Cellular viability was measured 48 hours after transfection using a CellTiter-Blue® assay. Exposure to XAV-939 significantly reduced cellular viability of SW837 but not SW480. (C) Cells where pre-incubated with XAV-939 and irradiated. Exposure to XAV-939 led to a significant radio sensitization of SW480 (1 μM P = 0.0116, 4 μM P = 0.0112; ANOVA model) and SW837 (5 μM P = 0.0223; 10 μM P = 0.000264; ANOVA model). The increased sensitivity to RT was stronger with higher doses of XAV-939. Each experiment was repeated three times. Data are displayed as mean values, n = 3, error bars {plus minus} SEM.