This scoping review systematically examines the application of Intervention Mapping (IM) in knowledge translation (KT) processes, focusing on its effectiveness, completeness, advantages and challenges. Following Joanna Briggs Institute (JBI) guidelines, we conducted a scoping review of 57 studies (1998–2024) from PubMed, Embase, Web of Science, and Chinese databases. The data extracted encompass the basic information of the studies, the objectives of the research, the part of the IM steps, as well as the main results and the methods used for evaluating. IM demonstrated broad applicability across 18 healthcare domains, with the highest prevalence in maternal and child health (12.3