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    一种鳗弧菌野生毒株的无标记基因缺失减毒突变株及其应用

    PL2009521328B2
    发明人
    马悦, 张元兴, 赵东玲, 王蓬勃
    受让人
    华东理工大学
    申请人
    Yang-Wan Kim, Choon-Yul Oh
    申请号
    027842
    申请日
    2021-10-11
    公开(公告)号
    PL2009521328B2
    公开(公告)日
    2019-07-30
    IPC分类号
    C09J007/02A61L015/58C09J153/02C09J191/00A61F013/02A61L015/14B32B005/02B32B015/04B32B027/04B32B007/12C09J007/04
    CPC分类号
    -
    优先权号
    134216
    优先权日
    2010-12-23
    摘要

    NOVELTY - The centrifuge has a rotor (8) for holding a microtiter plates with an opening directed outwardly. A motor rotates the rotor around a rotation axis. A housing (23) has a cylindrical inner surface, where a drain (30) is provided for discharging fluid expelled from the microtiter plates. A gap is provided between the inner surface and rotor such that by rotating the rotor, a wind is generated that drives the expelled fluid on the inner surface to the drain. An aspiration pump is connected to the drain for discharging fluid.

    USE - Centrifuge for cleaning reaction vessel unit.

    ADVANTAGE - The rod has a protrusion located above the portion of rod within the vessel that prevents the rod from entering into the vessel and from touching the bottom walls of the vessel. The aspiration pump is connected to the drain of the centrifuge allows a faster and improved clearing of the housing and also avoids the cross-contaminations based on sample liquid present on the walls and bottom of the housing of the centrifuge. The method for centrifuging the microtiter plates allows a quick and clear emptying of the reaction vessel without the risk of any spillover together with an easy collection of the discharged sample liquid. The assays are blood typing by the microtiter plates, cellular assays where assays has magnetic beads with an oil overlay in order to ensure the formation of two separate phases guaranteeing a full coverage of the vessel. The dispensing fluid is kept heated in the reservoir where dispensing of the heated washing solution improves the washing efficiency. The opening of the reaction vessel directed radially inwards the unwanted residual liquid that is removed from the rod before transferring to the next reaction vessel thus the amount of unwanted transferred residual liquid is reduced resulting in improved reaction conditions.

    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:

    (1) a centrifuge for centrifuging a reaction vessel unit;

    (2) a method for centrifuging a reaction vessel unit;

    (3) a method for parallel testing by a gel separation;

    (4) a method for carrying out an assay by a magnetic beads; and

    (5) a method for carrying out an assay by a rod system.

    DESCRIPTION OF DRAWING(S) - The drawing shows a front view of a rotor and housing without a front side wall.

    Rotor (8)

    Base walls (12)

    Housing (23)

    Drain (30)

    Support (31)

    权利要求
    1 . A polymer blend comprising at least two immiscible polymers and a carbon nanotube pulp comprising entangled carbon nanotubes characterized as having one or more of the following characteristics: (i) a diameter of between about 10-100 nm, (ii) a length of between about 0.1-10 mm, (iii) a density of between about 0.3-1.9 g/cm 3 , (iv) an aspect ratio of at least about 250,000, (v) a strain to failure of between about 1.8-7%, and (vi) a surface area from about 100-300 m 2 /g.
    2 . The polymer blend of claim 1 , wherein the carbon nanotubes are non-functionalized.
    3 . The polymer blend of claim 1 , wherein the carbon nanotubes are functionalized.
    4 . The polymer blend of claim 1 , wherein the carbon nanotube pulp is present in an amount of between about 0.001% by weight to about 20% by weight, based on the total weight of the immiscible polymers.
    5 . The polymer blend of claim 4 , wherein the carbon nanotube pulp is present in an amount of between about 0.5% by weight to about 5% by weight, based on the total weight of the immiscible polymers.
    6 . The polymer blend of claim 1 , wherein a first immiscible polymer and a second immiscible polymer are selected from: polyethylene and polyester, polystyrene and polyethylene, maleic anhydride grafted polystyrene and polyethylene, ethylene vinyl acetate and polyvinyl chloride, maleic anhydride grafted polyvinyl chloride and ethylene vinyl acetate, polyvinyl chloride and polycarbonate, polyvinyl chloride and polymethyl methacrylate, polymethyl methacrylate and polyvinyl chloride, styrene acrylonitrile and polycarbonate, polycarbonate and polycaprolactam, polycarbonate and polypropylene, polycarbonate and polyethylene, ethylene vinyl acetate and polypropylene, polyethylene and polypropylene, polyethylene and polystyrene, acrylonitrile butadiene styrene copolymer and polyvinylchloride, polyethylene and polyvinylchloride and polystyrene and polyvinylchloride.
    7 . The polymer blend of claim 6 , wherein the first immiscible polymer is present in an amount of about 10%-90% by weight and the second immiscible polymer is present in an amount of about 90%-10% by weight, based on the total weight of the immiscible polymers.
    8 . The polymer blend of claim 1 , wherein a total amount of immiscible polymers present in the polymer blend is between about 5% by weight to about 99.9% by weight, based on the total weight of the polymer blend.
    9 . The polymer blend of claim 1 , further comprising at least one of a surfactant, an electrically conductive filler, an impact modifier, antioxidant, nucleating agent, coupling agent, UV absorber, UV stabilizer, pigment, dye, reinforcing filler, slip agent, plasticizer, processing aid, lubricant, viscosity control agent, tackifier, anti-blocking agent, extender oil, metal deactivator, voltage stabilizer, flame retardant filler, booster, catalyst, smoke suppressant, mold release agent and non-conductive filler.
    10 . A method of forming a polymer blend comprising: obtaining a carbon nanotube pulp, dispersing a first portion of a carbon nanotube pulp into a first immiscible polymer to form a first blend, dispersing a second immiscible polymer into a second portion of the carbon nanotube pulp to form a second blend, and mixing the first blend and the second blend to form the polymer blend wherein the carbon nanotube pulp comprises entangled carbon nanotubes characterized as having one or more of the following characteristics: (i) a diameter of between about 10-100 nm, (ii) a length of between about 0.1-10 mm, (iii) a density of between about 0.7-1.9 g/cm 3 , (iv) an aspect ratio of at least about 250,000, (v) a strain to failure of between about 1.8-7%, and (vi) a surface area from about 100-300 m 2 /g.
    11 . The method of claim 10 , wherein the first immiscible polymer is melt mixed into the first portion of a carbon nanotube pulp, the second immiscible polymer is melt mixed into the second portion of the carbon nanotube pulp and the first blend is melt mixed into the second blend.
    12 . A polymer blend of claim 1 for use in flame retardant applications, high impact applications and conducting applications.
    13 . A pellet comprising the polymer blend of claim 1 .
    14 . An article comprising the polymer blend of claim 1 .
    说明书
    [0001]BACKGROUND OF THE INVENTION
    [0002]1. Field of the Invention
    [0003]The invention relates to DC/DC regulation using NMOS and PMOS transistors as pass devices, and more particularly to the use of two output voltage regulation loops, where a current sense buffer triggers the second voltage regulation loop.
    [0004]2. Description of the Related Art
    [0005]Linear regulators are used in many electronic devices and applications for converting an unregulated input voltage, typically a low voltage input, to a regulated output voltage. One particular implementation of a linear voltage regulator is referred to as a low dropout (LDO) regulator. Such a LDO regulator is a DC linear voltage regulator, it generally operates with a very small input-output differential voltage across the regulator and offers a well regulated voltage at its output terminal. Usually a LDO regulator consists of a feedback-controlled loop connected to a transistor (or transistors). The feedback-controlled loop typically comprises a differential amplifier (error amplifier). The feedback-controlled loop controls the gate voltage of the transistor and thereby its impedance. Depending on the gate voltage, the transistor supplies a different amount of current to the LDO's output terminal. The gate voltage is modulated such that the regulator provides a steady DC voltage regardless of load conditions or input transients.
    [0006]FIG. 1 shows a circuit of the conventional art including an NMOS pass transistor 31 and a PMOS pass transistor 32 , a voltage divider 35 , and error amplifiers 33 and 34 using a common Vref input. NMOS pass transistor 31 and PMOS pass transistor 32 are coupled in parallel between voltage input Vin and voltage output Vo. Coupled between Vo and ground is voltage divider 35 with resistors 351 , 352 , and 353 . The junction V FB1 between resistors 351 and 352 is coupled to the +input of error amplifier 34 . The junction V FB2 between resistors 352 and 353 is coupled to the −input of error amplifier 33 . The outputs of error amplifiers 33 and 34 drive the gate G of NMOS pass transistor 31 and PMOS pass transistor 32 , respectively. The disadvantages of the circuit of FIG. 1 are:
    [0007]The circuit of FIG. 1 , with the two feedback voltages V FB1 and V FB2 , has the NMOS pass device always on when V FB1 >V FB2 . When V FB2 ≦V REF , the PMOS is turned on only when the output voltage Vo drops such that V FB1 ≦V REF . The disadvantage of this circuit is that it is very dependent on the offset of the two error amplifiers 33 and 34 and on the accuracy of the voltage divider 35 to minimize the output drop voltage necessary to turn the PMOS on. This circuit will basically have two possible output voltages Vo, depending on the output load current.
    [0008]FIG. 2 is another circuit of the conventional art and similar to FIG. 1 , except that two reference voltages VR 1 , VR 2 and two resistors 41 , 42 are used. Its disadvantages are: The circuit of FIG. 2 has only one feedback voltage but still two error amplifiers 33 and 34 to drive the NMOS 31 and PMOS 32 pass devices. The output voltage Vo is regulated in this case to the same value from the two error amplifiers 33 and 34 but a small offset between the two regulating (feedback) loops will make the circuit unstable.
    [0009]U.S. patent applications and U.S. Pat. Nos. which have a bearing on the present invention are:
    [0010]U.S. Patent Application 2009/0189577 (Lin et al.) describes an LDO linear regulator including a PMOS power transistor having a variable size, where its size is adjustable by a control signal. The control signal is an N-bit digital signal generated by an analog-to-digital converter. In addition a variable current source can be used, driven by the same analog-to-digital converter.
    [0011]U.S. Patent Application 2009/0115382 (Hasegawa et al.) discloses a Low Drop-Out/Linear Drop-Out regulator having a PMOS output transistor Tr 1 , an error amplifier, a buffer amplifier and a drive capability adjustment transistor PMOS Tr 3 . A second PMOS transistor Tr 2 provides feedback to the buffer amplifier.
    [0012]U.S. Pat. No. 7,521,909 (Dow et al.) shows a linear regulator comprising a pass element, transistor 24 , an error amplifier 23 , a buffer 33 , a sense network 28 (a voltage divider) and a Miller compensation circuit 40 . Transistor 24 is formed to include a main transistor which forms a sense current that is representative of the current through transistor 24 .
    [0013]U.S. Pat. No. 6,229,289 (Piovaccari et al.) teaches a regulator which switches between a switched mode and linear regulator (LDO) mode. The linear regulator controls a first transistor coupled between input Vin and output Vout. The switched mode controller, a Pulse Width Modulation controller, controls a second transistor which, in series with an inductor, is also coupled between input Vin and output Vout.
    [0014]U.S. Pat. No. 7,531,996 (Yang et al.) presents an LDO which includes an NMOS and a PMOS transistor coupled in parallel between supply power and output. First and second error amplifiers drive the NMOS and the PMOS transistor, respectively. A voltage divider provides the input(s) to the error amplifiers.
    [0015]It should be noted that none of the above-cited examples of the related art provide the advantages of the below described invention.
    [0016]SUMMARY OF THE INVENTION
    [0017]It is an object of at least one embodiment of the present invention to provide a method and a circuit to minimize the dropout voltage of a transistor pass device in a low dropout voltage regulator, while accommodating low and high current loads.
    [0018]It is another object of the present invention to provide a DC/DC regulator with good load transient regulation without the need of an external load capacitor.
    [0019]It is yet another object of the present invention to provide good load transient response.
    [0020]It is still another object of the present invention to provide chip area reduction.
    [0021]It is a further object of the present invention is to provide a low quiescent current.
    [0022]It is yet a further object of the present invention is to provide increased bandwidth of the regulation loop.
    [0023]It is still a further object of the present invention is to require a much smaller compensation capacitor.
    [0024]These and many other objects have been achieved by using a first and a second output voltage regulation loop where the first output voltage regulation loop uses an NMOS transistor as the pass device and the second output voltage regulation loop uses a PMOS transistor as the pass device. The NMOS transistor is used for small current loads up to 1 mA and the PMOS transistor is used for larger loads from 1 mA and up to maximum current load Imax. The first output voltage regulation loop comprises the NMOS transistor, a voltage divider and an error amplifier, the output of which drives the gate of the NMOS transistor. The second output voltage regulation loop comprises the PMOS transistor, the same voltage divider and error amplifier and a current sense buffer. One input of the current sense buffer couples to the output of the error amplifier. The other input of the current sense buffer senses the current through the NMOS transistor. The output of the error amplifier regulates the voltage at the gate of the NMOS transistor and the output of the current sense buffer regulates the gate voltage of the PMOS transistor when the current through NMOS transistor exceeds a specified threshold.
    [0025]For low currents (1 mA or less) the NMOS transistor acts as source follower. The error amplifier and the NMOS transistor are the master of the output voltage regulation loop. For higher currents (1 mA or more) the NMOS transistor acts as a current source delivering the maximum current of 1 mA. The voltage at the gate of the NMOS transistor is frozen and the rest of the current is delivered by the PMOS transistor. The current sense buffer together with the PMOS transistor become the master of the regulation output voltage.
    [0026]These and many other objects and advantages of the present invention will be readily apparent to one skilled in the art to which the invention pertains from a perusal of the claims, the appended drawings, and the following detailed description of the preferred embodiments.
    [0027]In the following, first and second conductivity types are opposite conductivity types, such as NMOS (n-channel) and PMOS (p-channel) transistors.
    [0028]BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a first circuit diagram of a conventional LDO as described above. FIG. 2 is a second circuit diagram of a conventional LDO as described above. FIG. 3 is a circuit diagram of the preferred embodiment of the present invention. FIG. 4 is a block diagram of the preferred method of the present invention.
    [0029]DESCRIPTION OF THE PREFERRED EMBODIMENT
    [0030]Referring now to FIG. 3 we describe the preferred embodiment of the present invention. Domino voltage regulator 30 comprises an NMOS transistor 31 (N 1 ), with gate G, source S and drain D, a PMOS transistor 32 (P 1 ), with gate G, source S and drain D, an error amplifier 33 (E 1 ), an Isense buffer 34 , and voltage divider comprising resistors 35 (R 1 ) and 36 (R 2 ). The drain D and source S of transistor 31 (N 1 ) are coupled between a positive power supply terminal with input voltage Vin and output voltage Vout. Similarly, source S and drain D of transistor 32 (P 1 ) are coupled between input voltage Vin and output voltage Vout. Error amplifier 33 (E 1 ) has its +terminal coupled to a reference voltage Vref and its output voltage Vg 1 coupled to the gate G of transistor 31 . A first input of Isense buffer 34 is coupled to source S of transistor 31 , a second input of Isense buffer 34 is coupled to the output of error amplifier 33 . The output of Isense buffer 34 (voltage Vg 2 ) is coupled to the gate G of transistor 32 . Resistors 35 and 36 are coupled between Vout and the power supply return terminal, typically ground. The junction of resistors 35 and 36 is coupled back to the −input of error amplifier 33 . Also shown coupled to Vout are external capacitor 37 (Cload) and load current 38 (Iload).
    [0031]In Domino voltage regulator 30 , transistor 31 (N 1 ) and transistor 32 (P 1 ) are the pass devices. Transistor 31 is used for very small load currents, transistor 32 is used only for higher load currents in parallel with transistor 31 . There are two output voltage regulation loop configurations:
    [0032]the “low current loop”, where error amplifier 33 and transistor 31 are the masters, and the “high current loop”, where Isense buffer 34 and transistor 32 are the masters.
    [0033]Domino voltage regulator 30 works as a DC/DC regulator, it has good load transient regulation response even when no external load capacitor 37 is used.
    [0034]For small load current of between about 0 to 1 mA, transistor 31 is used and the load transient response is guaranteed by transistor 31 working as a source follower. In this configuration error amplifier 33 and transistor 31 are the master of the output voltage regulation loop. For higher load currents of between about 1 mA and Imax, transistor 31 works as current source only, delivering its specified maximum current (about 1 mA in this case). Voltage Vg 1 , as applied to gate G of transistor 31 and to Isense Buffer 34 , is fixed and the rest of the current is delivered by transistor 32 . In this configuration, the Isense buffer 34 becomes together with transistor 32 the master of the regulation output voltage. The passing of the control from the low current loop to the high current loop is like one Domino piece affecting the next Domino piece. The load transient response as well as the increased band-width of the regulation loop is guaranteed in this case by transistor 32 . This is guaranteed because in a standard linear regulator with a PMOS transistor as pass device, the load transient regulation is driven only by the regulation loop and its band-width. This means increasing the band-width improves also the load transient response. The band-width increase costs current, however this is not possible in a circuit where the quiescent current is required to be as small as possible, especially when the load current is close to zero. In the present circuit the regulation loop of the PMOS transistor starts to contribute when the load current is already sufficient high to make the band-width and the quiescent current increase acceptable. The limiting factor of this circuit is that the minimum output voltage Vout is limited by the threshold voltage Vth of NMOS transistor 31 .
    [0035]We now describe the function of Isense Buffer 34 :
    [0036]Assuming the NMOS pass device N 1 contributes to the load Domino voltage regulator 30 up to 1 mA. The Isense Buffer 34 measures the current flowing in N 1 by biasing another NMOS transistor, called N Isense , with the same gate, source and drain voltage of N 1 , Vg 1 , V out and V IN , respectively. Assume that N Isense is a factor 1/1000 smaller than N 1 . This means that when 1 mA current flows through N 1 , 1 uA current flows through N Isense . A current comparator now compares the current flowing in N Isense to a constant bias current, 1 uA in this case. The output of this current comparator regulates the gate voltage Vg 2 , of the PMOS pass device P 1 .
    [0037]In another description of the preferred embodiment of the present invention, and referring again to FIG. 3 , the Domino voltage regulator 30 features:
    [0038]1. A first output voltage regulation loop comprising a first switching means 31 (N 1 ) of a first conductivity type, a first resistive means 35 (R 1 ) and an amplifier 33 (E 1 ), where an output (Vg 1 ) of the amplifier is coupled to the control gate (G) of the first switching means, where the first output voltage regulation loop controls an output voltage at a junction Vout between the first switching means and the first resistive means, the first output voltage regulation loop controlling small currents of the first switching means. 2. A second output voltage regulation loop comprising a second switching means 32 (P 1 ) of a second conductivity type, the first resistive means 35 (R 1 ), the amplifier 33 (E 1 ), and a current sense buffer 34 (Isense buffer), where an output of the current sense buffer (Vg 2 ) is coupled to a control gate (G) of the second switching means, where the current sense buffer senses a current flowing in the first switching means and regulates the gate voltage of the control gate of the second switching means when the current flowing in the first switching means exceeds a specified threshold voltage, where the second output voltage regulation loop controls the output voltage at the junction Vout, the second output voltage regulation loop controlling large currents of the second switching means. 3. A second resistive means 36 (R 2 ) coupled between a power supply return terminal (typically ground) and a first input (−) of the amplifier. The second input (+) of the amplifier is coupled to a reference voltage Vref.
    [0039]The first switching means is a NMOS transistor having its drain-source path (D-S) coupled between power supply Vin and junction Vout. The second switching means is a PMOS transistor having its source-drain path (S-D) coupled between power supply Vin and junction Vout. The first resistive means is coupled between junction Vout and the first input (−) of amplifier 33 which has a minus polarity. The second input (+) of amplifier 33 , which has a plus polarity, is coupled to reference voltage Vref.
    [0040]A first input of current sense buffer 34 is coupled to the output of amplifier 33 and a second input of the current sense buffer is coupled to the source S of transistor 31 (equal to junction Vout). The output Vg 2 of the current sense buffer is, as already mentioned earlier, coupled to the gate G of PMOS transistor 32 . For small currents ranging from between about 0 mA and a maximum of about 1 mA, a load transient response is guaranteed by the first switching means working as a source follower where amplifier 33 and first switching means 31 are the master of the first output voltage regulation loop.
    [0041]For high currents ranging from between about 1 mA and a maximum current Imax the first switching means acts as a current source only and delivers in this instant a maximum current of about 1 mA. At high currents, the voltage at the control gate G of first switching means 31 is fixed and currents ranging from about 1 mA to a maximum current Imax are delivered by second switching means 32 ; then current sense buffer 34 together with second switching means 32 become the master of the second output voltage regulation loop.
    [0042]Switching means may imply devices such as a transistor or a transistor circuit, either of these in discrete form or in integrated circuits (IC), a relay, a mechanical switch. These devices are cited by way of illustration and not of limitation, as applied to switching means.
    [0043]Resistive means may imply devices such as resistors, transistors or transistor circuits, either of these in discrete form or in integrated circuits (IC), functioning as resistors. These devices are cited by way of illustration and not of limitation, as applied to resistive means.
    [0044]Advantages
    [0045]Advantages of the present invention are:
    [0046]Good load transient regulation without the need of an external load capacitor resulting in reduced cost to customer. Area reduction of the IC die, because of the less critical stability of the Line regulators. Miller compensation capacitors are not required. Low quiescent current: this circuit optimizes the quiescent current required by a Line regulator which does not use an external load capacitor to improve its load transient response. Eliminating the external load capacitors of the Line regulators has two main advantages: One is application board related, because it cuts the costs and reduces the routing complexity. The other one is Silicon die area related. An external load capacitor at the Line regulator output introduces stability problems to the circuit witch need to be compensated by an internal Miller compensation capacitor to become stable.
    [0047]We now describe with reference to FIG. 4 a preferred method of providing a voltage regulator with a voltage regulation loop for small current loads and a voltage regulation loop for high current loads:
    [0048]Block 1 provides a first output voltage regulation loop comprising an NMOS transistor for small current loads to regulate an output voltage; Block 2 senses the current through the NMOS transistor by a current sense buffer; Block 3 regulates the gate voltage of a PMOS transistor by the current sense buffer; and Block 4 regulates the output voltage for high current loads by switching to a second output voltage regulation loop, comprising the PMOS transistor, while the NMOS transistor acts as a source follower.
    [0049]While the invention has been particularly shown and described with reference to the preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made without departing from the spirit and scope of the invention.
    同族专利
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