Conventional cotton fabrics used in hospitals and sportswear are prone to bacterial adhesion and proliferation, complicating hygiene and posing a health hazard. Modified cotton materials are in demand in healthcare and the sport industries due to the ability to impart various properties, including antipathogenic activity. In this work, cotton fabrics coated with hybrid boron nitride_zinc oxide (BN_ZnO) and diethylenetriamine (DETA)-modified BN_ZnO nanoparticles (BN_ZnO_DETA NPs) were studied. The presence of an amino layer on the BN_ZnO NP surface made it possible to deposit approximately twice as many BN_ZnO NPs, as well as to increase their stability (expressed as % of retained NPs) on the textile surface after immersion in water for 24 h from 63.6 to 92.6 %. Surface-modified textile materials demonstrated high hydrophobicity and associated increased resistance to various liquid contaminants (ink, soy sauce, and tomato juice), as well as self-cleaning ability under the influence of UV radiation. The pathogens (hospital E. coli U20 and S. aureus MW2 strains) were complete eliminated by fabric after 24 h of cultivation with the samples (BN_ZnO) and (BN_ZnO) + diethylenetriamine (DETA)) with bacteria. In vivo tests demonstrated the safety of BN_ZnO_DETA NPs-modified samples. Their contact with skin did not cause inflammation, and histological examination showed the structure of normal skin.

We developed electrospun polycaprolactone (PCL) membranes containing 1-5 wt % ZnO nanoparticles (NPs), the surface of which was plasma-functionalized with carboxyl groups for covalent immobilization of chlorhexidine (CHX) via carbodiimide chemistry. Quantum-chemical analysis revealed that carboxyl functionalization, followed by 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) activation, significantly enhanced CHX sorption, while ZnO NPs promoted charge transfer and increased the electrophilicity of the PCL matrix. The PCL-3%ZnO-CHX membranes exhibited high tensile strength (18.6 MPa), improved wettability, and prolonged Zn2+ release. The optimized composition demonstrated potent antibacterial and antifungal activity, achieving a 6-log reduction against S. aureus, E. faecium, E. coli, A. baumannii, and C. auris. In vitro studies confirmed excellent cytocompatibility toward human dermal fibroblasts, keratinocytes, and T-lymphocytes, with cell viability remaining above 93% throughout the study. Histological evaluation showed no chronic inflammation, necrosis, or foreign body reaction, while tissue organization in the PCL-3%ZnO-CHX group was comparable to the control. In a mouse tail amputation model, this material reduced blood loss and bleeding time by 6.6- and 1.5-fold, respectively. These results demonstrate that PCL-3%ZnO-CHX membranes combine mechanical strength, broad-spectrum antimicrobial activity, biocompatibility, immunocompatibility, and hemostatic performance, making them promising materials for wound dressings and tissue engineering.

Purulent-inflammatory skin and soft tissue disorders are widespread and costly for healthcare. Biofilm infections are typically chronic in nature because biofilm-residing bacteria very resistant to the immune system, antibiotics, and other treatments. Moreover, the pathogenic biofilm is a reservoir of pathogenic bacteria, constantly infecting the inflammatory region. Thus, long-term inflammation slows down healing and can lead to wounds that are difficult to treat. Here, plasma deposition coated polycaprolactone fibers with amine plasma polymers, followed by epoxy functionalization and attachment of ciprofloxacin, were created. Using XPS modeling, we estimated that the drug covered 20

Zinc oxide (ZnO) nanoparticles (NPs) have been investigated for various skin therapies in recent years. These NPs can improve the healing and modulate inflammation in the wounds, but the effective and biosafety concentration in such changes are yet to be known. In this study, we have designed antibacterial, superabsorbent, and hemostatic composite dressings produced through the lyophilization of a curdlan and chitosan polymer combination, incorporating ZnO nanoparticles (NPs) at concentrations of X = 1, 3, 5, and 7 wt% (CUR/CS_ZnO-X%). ZnO nanoparticles are uniformly dispersed within the obtained materials. An increase in ZnO NP concentration correlates with an enhancement in the specific surface area of the composite foams, attributed to the creation of larger pores. Despite all samples demonstrating identical capacity to absorb DMEM cell media (1500 wt%), the swelling rate of the materials escalated with the incorporation of zinc particles and attained a 1500 wt% DMEM media per 9 s. Antibacterial assays demonstrated that CUR/CS_ZnO-3 % entirely inhibited Staphylococcus aureus CSA154, Escherichia coli U20, and Klebsiella pneumoniae C324/23 strains for 24 h, while diminishing the colony-forming units (CFUs) of Clostridium perfringens D46 strain, responsible for gangrene, and the Acinetobacter baumannii C66627/23 strain by 99 %. All samples demonstrated excellent hemostatic properties: the measured blood coagulation indexes of samples were determined in diapason 14-32 %.

Developing efficient and reliable technology for modifying the orthopedic implant surface to impart antibacterial properties is crucial for reducing the risk of implantation-associated infection. For the first time, Ag+, Cu2+ and Zn2+ ion release kinetics and antibacterial activity of Ag-, Cu-, and Zn-containing coatings deposited on 3D-printed Ti6Al4V alloy by plasma electrolytic oxidation (PEO), anodization (ANO) and cathodic electrodeposition (CD) were compared against Gram-positive Staphylococcus aureus (S. aureus) ATCC 29213 using different in vitro models (application of a bacterial suspension droplet to the sample surface, biofilm formation (SEM analysis and quantification of S. aureus biofilm by crystal violet staining), and planktonic growth) in a nutrient medium and physiological solution (PS, 0.9 % NaCl). A separate sample group was tested in PS against Gram-negative Escherichia coli (E. coli) K261 and Candida albicans (C. albicans) C324/23. After 24 h, concentration of released ions was 0.07-0.31 ppm (Ag+), 0.3-0.41 ppm (Cu2+), and 0.58-0.62 ppm (Zn2+), and in order of increasing bactericidal effect, the ions can be arranged as Zn2+ < Cu2+ < Ag+, with the effective Ag+ concentration being almost an order of magnitude lower than those of Zn2+ and Cu2+. For Ag-containing samples, S. aureus was completely inactivated in PS within 3 h, while a 99.0 % reduction (2-log) was achieved in nutrient medium. After 24 h, effects ranged from complete growth inhibition to a 99.0-99.9999 % reduction. Ag-containing coatings also completely inactivated E. coli K261 and C. albicans C324/23 after 3 h at initial cell concentrations in PS of 10(8) and 10(6) CFU/mL, respectively. The results of various in vitro models are discussed considering the testing environment (nutrient medium or physiological solution) and possible in vivo conditions of occurrence of surgery-related and postoperative infections. The use of PEO, ANO and CD methods for depositing bactericidal coating is a promising approach that does not require expensive equipment, is easily scalable and can be integrated into the production chain of orthopedic implants for personalized medicine.

Novel nanomaterials used for wound healing should have many beneficial properties, including high biological and antibacterial activity. Immobilization of proteins can stimulate cell migration and viability, and implanted Ag ions provide an antimicrobial effect. However, the ion implantation method, often used to introduce a bactericidal element into the surface, can lead to the degradation of vital proteins. To analyze the surface structure of nanofibers coated with a layer of plasma COOH polymer, fibronectin/gentamicin, and implanted with Ag ions, a new X-ray photoelectron spectroscopy (XPS) fitting method is used for the first time, allowing for a quantitative assessment of surface biomolecules. The results demonstrated noticeable changes in the composition of fibronectin- and gentamicin-modified nanofibers upon the introduction of Ag ions. Approximately 60% of the surface chemistry has changed, mainly due to an increase in hydrocarbon content and the introduction of up to 0.3 at.% Ag. Despite the significant degradation of fibronectin molecules, the biological activity of Ag-implanted nanofibers remained high, which is explained by the positive effect of Ag ions inducing the generation of reactive oxygen species. The PCL nanofibers with immobilized gentamicin and implanted silver ions exhibited very significant antipathogen activity to a wide range of Gram-positive and Gram-negative strains. Thus, the results of this work not only make a significant contribution to the development of new hybrid fiber materials for wound dressings but also demonstrate the capabilities of a new XPS fitting methodology for quantitative analysis of surface-related proteins and antibiotics.

Zn-containing TiO2-based coatings with Na, Ca, Si, and K additives were obtained by plasma electrolytic oxidation (PEO) of Ti in order to achieve an effective and broad bactericidal protection without compromising biocompatibility. A protocol has been developed for cleaning the coating surface from electrolyte residues, ensuring the preservation of the microstructure and composition of the surface layer. Using high-resolution transmission electron microscopy, three characteristic microstructural zones in the PEO-Zn coating are well documented: zone 1 with a TiO2-based nanocrystalline structure, zone 2 with an amorphous structure, and zone 3 around pores with an amorphous-nanocrystalline structure. The excellent cytocompatibility of PEO-Zn samples was confirmed by three different methods: monitoring the proliferation of MC3T3-E1 cells, assessing the viability of sheep osteoblast cells using calcein-AM staining and fluorescence microscopy, and incubation with spheroids based on primary osteoblast cells and mouse embryonic fibroblast NIH3T3 cells. The PEO-Zn coatings absorb >60% of the incident light over the UV and Vis-NIR spectral ranges. After 24 h, the PEO-Zn coatings completely inactivate four types of strains: Gram-positive Staphylococcus aureus CSA154 and ATCC29213 and Gram-negative Escherichia coli K261 and U20, and also prevent E. coli U20 and K261 biofilm formation. The superior antibacterial activity is associated with the synergistic effect of Zn2+ ions in safe concentration and reactive oxygen species (ROS) generated in response to either UV irradiation or soft short-term X-ray irradiation. The X-ray irradiation-induced ROS formation by a PEO coating is reported for the first time. The enhanced bactericidal activity after X-ray irradiation compared to UV illumination is attributed to the more intense ROS generation in the first few hours. The results obtained significantly expand the possibilities of using PEO coatings on the surfaces of titanium implants.

Superelastic Ti-18Zr-15Nb alloy capable of mimicking the mechanical behavior of a bone tissue is a promising biomaterial but suffers from the lack of antibacterial properties. To address this problem, we developed a combined surface treatment method: formation of a porous sub-surface layer, deposition/precipitation of Au nanoparticles (AuNPs), surface functionalization with cysteine amino acid and grafting of gentamicin. The sizes distribution and AuNPs content were greatly effected by the synthesis methods. The AuNPs with an average size of 3 nm were obtained by precipitation from a AuNP colloidal solution. Larger AuNPs were formed by preliminary alloy functionalization in a NaBH4 solution followed by treatment in a HAuCl4 solution. Successful surface functionalization with L-cysteine and attachment of gentamicin were confirmed by XPS analysis. Due to the formation of stable cysteine-gentamicin complexes attached to AuNPs, the materials showed high antibacterial activity against Escherichia coli and Staphylococcus aureus strains. Better antibacterial properties are ascribed to fine isolated AuNPs as compared to larger ones. Cytocompatibility was assessed for osteoblast cells. In the case of smaller AuNPs, an accelerated restoration of osteoblastic cell proliferation and a more organized actin cytoskeleton were observed. Hemolytic activity of the developed materials was investigated. Functional mechanical properties were not affected by the surface treatment.

We report a one-pot plasma electrolytic oxidation (PEO) strategy for forming a multi-element oxide layer on the titanium surface using complex electrolytes containing Na2HPO4, Ca(OH)2, (NH2)2CO, Na2SiO3, CuSO4, and KOH compounds. For even better bone implant ingrowth, PEO coatings were additionally loaded with bone morphogenetic protein-2 (BMP-2). The samples were tested in vivo in a mouse craniotomy model. Tests for bactericidal and fungicidal activity were carried out using clinically isolated multi-drug-resistant Escherichia coli (E. coli) K261, E. coli U20, methicillin-resistant Staphylococcus aureus (S. aureus) CSA154 bacterial strains, and Neurospora crassa (N. crassa) and Candida albicans (C. albicans) D2528/20 fungi. The PEO-Cu coating effectively inactivated both Gram-positive and Gram-negative bacteria at low concentrations of Cu2+ ions: minimal bactericidal concentration for E. coli and N. crassa (99.9999%) and minimal inhibitory concentration (99.0%) for S. aureus were 5 ppm. For all studied bacterial and fungal strains, PEO-Cu coating completely prevented the formation of bacterial and fungal biofilms. PEO and PEO-Cu coatings demonstrated bone remodeling and moderate osteoconductivity in vivo, while BMP-2 significantly enhanced osteoconduction and osteogenesis. The obtained results are encouraging and indicate that Ti-based materials with PEO coatings loaded with BMP-2 can be widely used in customized medicine as implants for orthopedics and cranio-maxillofacial surgery.

Bone implants with biocompatibility and the ability to biomineralize and suppress infection are in high demand. The occurrence of early infections after implant placement often leads to repeated surgical treatment due to the ineffectiveness of antibiotic therapy. Therefore, an extremely attractive solution to this problem would be the ability to initiate bacterial protection of the implant by an external influence. Here, we present a proof-of-concept study based on the generation of reactive oxygen species (ROS) by the implant surface in response to X-ray irradiation, including through a layer of 3 mm adipose tissue, providing bactericidal protection. The effect of UV and X-ray irradiation of the implant surface on the ROS formation and the associated bactericidal activity was compared. The focus of our study was light-sensitive Si-doped TiCaCON films decorated with Fe and Pt nanoparticles (NPs) with photoinduced antibacterial activity mediated by ROS. In the visible and infrared range of 300-1600 nm, the films absorb more than 60% of the incident light. The high light absorption capacity of TiO2/TiC and TiO2/TiN heterostructures was demonstrated by density functional theory calculations. After short-term (5-10 s) low-dose X-ray irradiation, the films generated significantly more ROS than after UV illumination for 1 h. The Fe/TiCaCON-Si films showed enhanced biomineralization capacity, superior cytocompatibility, and excellent antibacterial activity against multidrug-resistant hospital Escherichia coli U20 and K261 strains and methicillin-resistant Staphylococcus aureus MW2 strain. Our study clearly demonstrates that oxidized Fe NPs are a promising alternative to the widely used Ag NPs in antibacterial coatings, and X-rays can potentially be used in ROS-regulating therapy to suppress inflammation in case of postimplant complications.

Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species and to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed two known sequence types and one new one that is described for the first time in this work. The results we obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected.

The objective of this research was to develop an environment-friendly and scalable method for the production of self-sanitizing electrospun nanofibers. This was achieved by immobilizing silver nanoparticles (Ag NPs) onto plasma-treated surfaces of biodegradable polycaprolactone (PCL) nanofibers. The plasma deposited polymer layer containing carboxyl groups played a critical role in providing a uniform distribution of Ag NPs on the nanofiber surface. Ag ions were absorbed by electrostatic interaction and then reduced under the action of UV-light. The concentration and release of Ag ions were analyzed using the EDXS/XPS and ICP AES methods, respectively. Although high levels of Ag ions were detected after 3 h of immersion in water, the material retained a sufficient amount of silver nanoparticles on the surface (~2.3 vs. 3.5 at.% as determined by XPS), and the release rate subsequently decreased over the next 69 h. The antipathogenic properties of PCL-Ag were tested against gram-negative and gram-positive bacteria, fungi, and biofilm formation. The results showed that the PCL-Ag nanofibers exhibit significant antimicrobial activity against a wide range of microorganisms, including those that cause human infections. The incorporation of Ag NPs into PCL nanofibers resulted in a self-sanitizing material that can be used in variety of applications, including wound dressings, water treatment, and air filtration. The development of a simple, scalable, and environmentally friendly method for the fabrication of these nanofibers is essential to ensure their widespread use in various industries. The ability to control the concentration and release rate of Ag ions in the PCL nanofibers will be critical to optimize their efficacy while minimizing their potential toxicity to human cells and the environment.

High-entropy coatings (HECs) are of a great interest for the protection of structural steels and alloys used in the costal and offshore areas. Here, thick, dense, and uniform Fe-Cr-Ni-Co-(Cu) coatings with a crack-free surface have been successfully deposited on AISI 420S steel by vacuum electro-spark deposition using CrNiCo and CrNiCoCu electrodes. The coatings consist of columnar grains (approximately 300 nm in diameter) and subgrains (10-50 nm thick) of an fcc phase and spherical inclusions of mixed SiO2 + (Cr,Ti)2O3 oxide, 30-50 nm in size. Although Cu is an element prone to segregation, the experimental results show that Cu does not form its own phase and is in the metal solid solution. Molecular dynamics simulation shows that Cu has a slight tendency to self-clustering and form Cu-rich clusters in FeCrNiCo-Cu HECs. However, several regions enriched in Cu are observed in the FeCrNiCo-Cu samples. FeCrNiCo coatings tested in artificial seawater and the Black Sea exhibited enhanced corrosion resistance. In tribocorrosion tests, FeCrNiCo-(Cu) coatings performed better than steel substrate due to faster recovery of a passive film. The addition of Cu has a positive effect on the antibacterial activity of FeCrNiCo coatings against Gram-positive B. cereus Arc30 and B. cereus F strains.

The use of nanoparticles (NPs) to modify the surface of cotton fabric is a promising approach to endowing the material with a set of desirable characteristics that can significantly expand the functionality, wear comfort, and service life of textile products. Herein, two approaches to modifying the surface of hexagonal boron nitride (h-BN) NPs with a hollow core and a smooth surface by treatment with maleic anhydride (MA) and diethylene triamine (DETA) were studied. The DETA and MA absorption on the surface of h-BN and the interaction of surface-modified h-NPs with cellulose as the main component of cotton were modeled using density functional theory with the extended Perdew-Burke-Ernzerhof functional. Theoretical modeling showed that the use of DETA as a binder agent can increase the adhesion strength of BN NPs to textile fabric due to the simultaneous hydrogen bonds with cellulose and BN. Due to the difference in zeta potentials (-38.4 vs -25.8 eV), MA-modified h-BN NPs form a stable suspension, while DETA-modified BN NPs tend to agglomerate. Cotton fabric coated with surface-modified NPs exhibits an excellent wash resistance and high hydrophobicity with a water contact angle of 135° (BN-MA) and 146° (BN-DETA). Compared to the original textile material, treatment with MA- and DETA-modified h-BN NPs increases heat resistance by 10% (BN-MA fabric) and 15% (BN-DETA fabric). Cotton fabrics coated with DETA- and MA-modified BN NPs show enhanced antibacterial activity against Escherichia coli U20 and Staphylococcus aureus strains and completely prevent the formation of an E. coli biofilm. The obtained results are important for the further development of fabrics for sports and medical clothing as well as wound dressings.

The spread of bacterial, fungal, and viral diseases by airborne aerosol flows poses a serious threat to human health, so the development of highly effective antibacterial, antifungal and antiviral filters to protect the respiratory system is in great demand. In this study, we developed ZnO-modified polycaprolactone nanofibers (PCL-ZnO) by treating the nanofiber surface with plasma in a gaseous mixture of Ar/CO2/C2H4 followed by the deposition of ZnO nanoparticles (NPs). The structure and chemical composition of the composite fibers were characterized by SEM, TEM, EDX, FTIR, and XPS methods. We demonstrated high material stability. The mats were tested against Gram-positive and Gram-negative pathogenic bacteria and pathogenic fungi and demonstrated high antibacterial and antifungal activity.

Climate change brings new risks of emergence of especially dangerous diseases. The paper reports the possibility of assessing the pathogenic potential of bacteria as demonstrated by studying the allelic polymorphism of anthrax bacterium pathogenicity factor genes, which is a prerequisite for assessing the associated microbiological risks. The allelic polymorphism of the capBCADE operon ( capB , capC , capA , capD , and capE genes) encoding the capsule biosynthesis proteins of Bacillus anthracis , and the acpA and acpB genes encoding the expression regulators of this operon have been studied for the first time. A number of single nucleotide polymorphisms (SNPs) were described in the strains of the studied sample, including 5 SNPs in the capB gene, 3 in capC , 4 in capA , 14 in capD , 2 in capE , and 15 in acpB , as well as 7 SNPs and one insertion in the acpA gene. As a result, the sample has been divided into sequence types for each gene and 17 genotypes, which are combinations of the identified sequence types. In silico translation of the detected alleles of the studied genes revealed three isoforms of the CapB and CapA proteins, two isoforms of the CapC and CapE proteins, six isoforms of the CapD protein, five isoforms of the AcpA protein, and four isoforms in the AcpB protein. It has been demonstrated that the SNP in the 351A → G position of capC is a marker of A.Br.Aust94 group strains. Based on the results, A.Br.Vollum group strains were divided into two subgroups. The strains in the evolutionary lines B and C differed from the line A strains by the presence of an 853G → A SNP in the acpA gene. In addition, a previously unknown variable number tandem repeat (VNTR), has been found in the acpA gene and the possibility of using it for differentiating and genotyping of B. anthracis strains has been demonstrated.

The history of mankind is inextricably linked with the fight against infections, and today this problem is even more urgent. To address this important issue, we deposited h-BN coatings decorated with ultrafine metallic silver or iron oxide nanoparticles (NPs). The coatings release metal ions in a concentration dependent manner. Fe/h-BN coatings at a minimum inhibitory concentration (MIC) of iron oxide NPs (74 mu g/cm(2)), effectively suppress the growth of Escherichia coli K-261 and U20, Staphylococcus aureus MW2 and 839, and Streptococcus pneumoniae ATCC33400 bacterial strains and Candida parapsilosis ATCC90018 fungus after 24 h. Enhanced bactericidal and fungicidal activities of Fe-rich samples is attributed to generation of high amount of ROS. Ag/h-BN materials at MIC of Ag equal to 12 mu g/cm(2), completely inactivate Escherichia coli K-261 and U20, and Staphylococcus aureus MW2 and 839 strains after 24 h. Sample with 25 mu g/cm(2) of Ag NPs kill all Escherichia coli K-261 and U20, and Staphylococcus aureus MW2 cells after 3 h and effectively inhibit the growth of Staphylococcus aureus ATCC25923, Candida parapsilosis ATCC90018, Escherichia coli C600, Candida auris CBS10913, and Streptococcus pneumoniae ATCC33400. These results are important for the further development of nanostructured films with a wide spectrum of activity against various pathogens.

The development of flexible and low-cost methods of surface functionalization to fight infection at the early stage is an urgent scientific task. Herein, polymerization in low-pressure plasma rich in COOH species and carbodii-mide chemistry methods were utilized to immobilize four different therapeutic agents (antibiotic (gentamicin), antimicrobial peptide (indolicidin), anti-adhesive molecules (heparin) and nitroxide radicals (2,2,5,5-tetramethyl-3-carboxyl-pyrrolidine-1-oxyl)) on the surface of nanostructured biocompatible TiCaPCON films to impart antibacterial characteristics. The polymers deposited from COOH-rich plasma showed decent stability in phosphate-buffered saline solution and were successfully used for the immobilization of different therapeutic agents via ionic or covalent bond. The bactericide attachment was proved by FTIR spectroscopy and XPS analysis. All samples with grafted therapeutic agents were hydrophilic with water contact angle values in the range of 26-56 degrees. Bactericide release tests indicated the maximum concentration of therapeutic agents in the case of ionic immobilization. In case of covalent immobilization, fast initial release observed over 24 h was followed by slower leaching in the next 24 h (indolicidin), 48 h (heparin), and 96 h (gentamicin). The pH-sensitive COOH plasma polymer degradation and gentamicin release were demonstrated. The bactericide-linked films showed noticeable reduction of the antibiotic-sensitive E. coli U20 strain and, except indolicidin-immobilized samples, effectively inhibited growth of the antibiotic-resistant E. coli K261 strain at their initial concentration of 10(4) CFU/mL. The films with nitroxide radicals not only exhibited the highest antibacterial activity against E. coli K261 cells (100% after 8 h), but also prevented the biofilm formation.

Fabrication of bactericidal yet bioactive and biocompatable coatings is still a challenge. Here we report on novel bioactive, bactericidal, antifouling, and cytocompatible TiO2-based multi-element coatings that are effective against multidrug-resistant pathogenes. Bioactive elements (Ca, P, Si, and B) were added from electrolytes during plasma electrolytic oxidation (PEO), after which the coatings were subjected to ion implantation with Fe and Cu ions. Careful selection and optimization of PEO modes allowed obtaining optimal porosity and desired content of bioactive elements. Doping with B or Si improved the proliferation and differentiation of MC3T3-E1 osteoblastic cell. In addition, these materials exhibited good mineralization ability when exposed to simulated body fluid. Band Si-containing PEO coatings, decorated with Cu nanoparticles, showed good cytocompatibility, strong antibacterial activity against Escherichia coli and Staphylococcus aureus multidrug-resistant strains and completely prevented S. aureus biofilm formation. The B-doped PEO coating also had a pronounced biocidal effect, which may be associated with the boron dissolution and the formation of boron oxide.