Polypyrrole-regulated Co9S8/nitrogen-doped carbon (NC@Co9S8-NC) hybrids with three-dimensional (3D) interconnected architectures were synthesized via surface modulation of amino acid-complexed cobalt sulfide (ACCS) precursors. The polypyrrole coating increases nitrogen doping from 8.03 at.% to 12.05 at.%. Meanwhile, in-situ polymerization of pyrrole on the ACCS complex directs the self-assembly of spherical superstructures, which are retained after carbonization. The resulting 3D network facilitates electrolyte penetration and accommodates volume changes during sodiation/desodiation, and is expected to promote the formation of a stable solid electrolyte interphase. Compared to Co9S8-NC, the optimized NC@Co9S8-NC electrode delivers a high reversible capacity of 165.9 mAh g−1 after 200 cycles at 2 A g−1, faster Na + diffusion coefficient (10 times), and reduced charge-transfer resistance (25.30 Ω versus 36.08 Ω). This work offers a maneuverable approach for improving the sodium-ion storage performance of transition-metal sulfide.

A castor oil-based polyether polyol (COP) with molecular weight comparable to that of a petroleum-based polyether polyol (PPG) was prepared via the oxypropylation of castor oil and propylene oxide. Such COP was utilized to fabricate flexible polyurethane foams (FPUs). A comparative study was conducted on foams derived entirely from PPG (FPU-0), with 50 % COP substitution (FPU-0.5), and entirely from COP (FPU-1.0). Results indicated that the introduction of COP significantly enhanced hydrogen bonding interactions between the ester groups in the soft segments and the imino groups in the hard segments, as confirmed by FT-IR, XRD and DFT calculations. These stronger hydrogen bonds promoted the physical cross-links between the soft segments and the hard segments. This led to a remarkable improvement in tensile strength (from 99.5 kPa to 124.2 kPa) and energy dissipation under cyclic compression (increasing from 2.2 kJ/m(3) to 5.2 kJ/m(3) at the 4th cycle) while maintaining a high fracture elongation (similar to 140 %). Owing to its high damping capacity and sustainable, bio-based composition, the COP-based FPU showed great potential for impact-absorbing applications such as protective packaging, automotive seating cushions, and sports equipment midsoles. This work elucidated the crucial role of ester groups in enhancing intermolecular interactions and improving the properties of FPU, providing a practical guidance for developing high-performance bio-based flexible polyurethane foams.

Amorphous carbon has emerged as a promising anode material for sodium ion batteries (SIBs), yet its sodium storage capacity is limited by the adsorption-insertion-filling mechanism. Simply doping with heteroatoms like nitrogen and sulfur has not adequately addressed this challenge. In this study, we prepare N, S doped defected carbon spheres decorated with Fe single atoms (SA-Fe@NSCS), which demonstrate rapid sodium ion diffusion kinetics and exceptional capacity performance. Benefiting from the high-level doping of N and S, beneficial Fe-N active sites and abundant defects, SA-Fe@NSCS maintained a reversible capacity of 237.3 mAh g- 1 after 400 cycles at 0.5 A g- 1 and showed a specific capacity of 256.2 mAh g- 1 and 181 mAh g- 1 at 0.2 and 2 A g- 1, with excellent rate capability and outstanding cycling stability.

Selenium-enriched oyster proteins were hydrolyzed using trypsin to obtain peptides with angiotensin-I-converting enzyme (ACE) inhibitory activity. The hydrolysate was purified by ultrafiltration and two-step reversed-phase high-performance liquid chromatography (RP-HPLC), yielding the most active fraction M4-2 (selenium content: 37.00 ± 0.56 mg/kg; IC50: 0.774 mg/mL, significantly lower than the IC50 of the crude hydrolysate, 2.801 mg/mL). This fraction was further analyzed by LC-MS/MS and molecular docking, leading to the identification of 91 selenium-containing peptide sequences. Two novel peptides, SeMFRTSSK and QASeMNEATGGK, showing strong binding affinities (−9.8 and −9.0 kcal/mol, respectively), were selected. Molecular docking revealed that SeMFRTSSK bound to key residues in the ACE active pocket via hydrogen bonds, whereas QASeMNEATGGK interacted with the Zn2+ active center. Cellular assays using EA.hy926 cells demonstrated that both peptides were non-cytotoxic at concentrations up to 0.25 mg/mL. At 0.025 mg/mL, SeMFRTSSK and QASeMNEATGGK enhanced cellular NO release by 202.65% and 273.45%, respectively, while suppressing Endothelin-1 (ET-1) secretion by 18.03% and 27.86%, compared to the blank control group. Notably, these peptides induced higher levels of NO release and greater suppression of ET-1 secretion than those in the captopril-treated positive control group. These findings support selenium-enriched oyster-derived peptides as potential natural antihypertensive ingredients.

N-doped carbon aerogels have garnered increasing research interest in the field of energy and environment due to their unique structural features. Organic dyes, which contain redox-active sites and act as pollutants, are attractive candidates for cathode materials in Li-ion batteries but still suffer from poor cycle stability and rate performance. Therefore, there is still a lack of an easy and effective approach to rationally design the pore structure of N-doped carbon aerogels for efficiently and stably trapping dye molecules and converting them into high-performance cathode materials. Herein, we propose an innovative strategy for preparing nitrogen-doped carbon aerogels with a well-defined micropore structure (MNCAs) for efficient adsorption of dye molecules, subsequently converting them into high-performance lithium-ion battery cathode materials. MNCAs were synthesized via Schiff-based polymerization using polyhedral oligomeric silsesquioxane (POSS) as a template, resulting in a carbon framework with well-defined micropores. Benefiting from their high specific surface area and well-defined micropore structure, MNCAs exhibited a maximum adsorption capacity at equilibrium of 2273 mg g−1 for indigo. Notably, the indigo@nitrogen-doped carbon aerogel composite (IDG@MNCAs) exhibits high specific capacity, outstanding cycling stability, and remarkable rate capability. The discharge specific capacity of IDG@MNCAs retains 89% of its capacity (120 mAh g−1) after 200 cycles at 100 mA g−1 and maintains 70% capacity retention after 1200 cycles at the higher current density of 1000 mA g−1, surpassing many recently reported organic cathode materials.

Organo-layered double hydroxides (OLDHs) are widely used as slow-release carriers for drugs but are rarely used in controlled drug release. To develop slow-release substrates of pretilachlor with excellent controlled release properties, OLDH hybrids were prepared by intercalating different masses of sodium dodecyl sulfate (SDS) into MgAl-LDHs. The underlying structural characteristics were analyzed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), scanning electron microscopy (SEM), and contact angle measurements. Also, the adsorption capacity and release properties of OLDHs were explored by batch adsorption isotherms, adsorption kinetics, and release kinetics to reveal the structural effects of OLDHs on the release of pretilachlor. The results showed that the release of pretilachlor from OLDHs was dominated by Fickian diffusion and was closely related to the adsorption capacity and permeability of the matrix. The intercalation of SDS enhanced the hydrophobicity of LDHs, resulting in a higher adsorption capacity for pretilachlor and a lower permeability. Furthermore, as the SDS loading amount increased from 0.5 to 4.0 g, the adsorption capacity of the OLDHs was further enhanced (k F value increased from 2.52 to 12.83), while the permeability (d 001/f oc value) reversed, decreasing from 0.21 to 0.09. The release kinetics demonstrated that the release rate of pretilachlor from OLDHs was significantly lower compared to pure LDHs and showed a continuous decrease with increasing SDS loading amounts. The release time of 50% (T 50) pretilachlor from OLDHs with SDS loading amounts of 25%, 50%, 100%, and 200% of the anion exchange capacity was 4.78, 8.28, 25.4, and 45.34 times longer than the release time from pure LDHs. This displayed a strong curvilinear relationship with the adsorption rate constant k F and permeability d 001/f oc and a good linear relationship with the added amount of SDS. These findings highlight the potential of OLDHs in preparing pretilachlor controlled release formulations.

Photocatalytic technology is extensively employed for the reductive removal of water contaminants; however, it contends with low catalytic efficiency and challenges in catalyst recovery. In this study, we propose integrating experimental procedures with artificial intelligence modeling to enhance the purification of Se(IV)-contaminated wastewater. We present an efficient, easily recyclable, and cost-effective strategy for photocatalyst fabrication. Specifically, we develop a novel method for Se(IV) removal by immobilizing TiO2/BiOBr onto glass fiber cloth surfaces using chitosan for Se(IV) reduction in aqueous solutions. The TiO2/BiOBr/cloth (TB-4/cloth) catalyst achieves a remarkable 99.2 % Se(IV) removal within 2 h under visible light and maintains excellent Se(IV) reduction photocatalytic activity (86.4 %) even after eight cycles, remaining easily reusable. Additionally, we develop two machine learning models, namely artificial neural network (ANN) and long short-term memory (LSTM), to validate the anticipated experimental outcomes. Both models exhibit high accuracy and predictive capability (R-2 > 0.99, RMSE < 0.03). This study introduces a novel approach that combines experimentation with artificial intelligence modeling, paving the way for future advancements in Se(IV)-contaminated wastewater purification methods.

The simultaneous removal of Cd(II) cation and Se(IV) oxoanion from water by adsorption remains a challenge due to their opposite charges. Herein, we impregnate sulfidated nanoscale zerovalent iron into porous shrimp shell-derived biochar to establish Fe(0) and FeS active sites for trapping Se(IV) and Cd(II), respectively. The resulting composite (SNZVI/BC0.3) exhibited adsorption capacities of 177.8 and 206.6 mg/g for Cd(II) and Se(IV) in the single-component system, respectively, at an initial pH of 5.0. In the binary system, these values became 128.4 and 282.8 mg/g. Meanwhile, SNZVI/BC0.3 could eliminate over 97 % of Se(IV) and 99 % of Cd(II) in real-world water samples with environmental level of these contaminants (1.0 mg/L). In addition, SNZVI/ BC0.3 reduced the bioavailable content of Se and Cd in waterlogged soil (pH = 6.6) from 2.531 and 4.921 mg/kg to 1.407 and 0.908 mg/kg, respectively. Characterization-based analysis indicated that the reduction of Se(IV) by Fe(0) and the displacement reaction between Fe(II) in FeS and Cd(II) were the main adsorption mechanisms for the Se(IV) and Cd(II) sequestration, respectively. Moreover, site energy distribution analysis supported the synergistic adsorption of Se(IV) and Cd(II) on SNZVI/BC0.3 via cation bridges formed by the captured Cd(II), which promoted the Se(IV) sequestration. This work provides a strategy for the simultaneous remediation of Cd(II) and Se(IV) in water and soil.

Excess existence of the selenite (Se(IV)) is hazardous to water resources. Therefore, a need still exists for the removal of Se(IV) from the wastewater in the renewable, environmentally friendly, and low-cost way. This paper presents a novel way to design a Z-type heterojunction of UIO-66-NH2/BiOBr using co-precipitation technology for efficiently removal of Se(IV) from wastewater using the adsorption-visible photocatalytic synergy. The UIO-66-NH2 presents an enhanced adsorption of Se(IV) due to the expose of abundant linker defects, as the active sites. The Z-type heterojunction (UB-2) showed 96.4% removal of Se(IV) at an initial concentration of 100mg/L under visible light conditions due to its higher charge transfer capacity and electron-hole separation ability. The photocatalytic removal rate of UB-2 toward Se(IV) was 4.12 times and 9.93 times higher than that of UIO-66-NH2 and BiOBr, respectively. UB-2 could be reused and showed a good removal capacity in real-world water samples. The used UB-2 was superior to the nano-selenium in the scavenging of superoxide radical and DPPH, revealing a potential of biological antioxidant. This work will be beneficial for the efficient decontamination of Se(IV)-containing wastewater.

This study investigated the transformation of an agricultural byproduct, pomelo peel, into an environmental purifier by crosslinking assisted surface amination process and its use in wastewater treatment application. Surface morphology and textural analysis showed that the functionalized surface-amidated pomelo peel (SA-PP) attained a lamellar structure with an average pore size of 6.52 nm and a specific surface area of 7.82 m2 g-1. Zeta potential measurements confirmed that SA-PP exhibits a positive surface charge within a pH range of 3-11. This surface characteristic facilitated the effective adsorption of anionic dyes by SA-PP, including amaranth red (Qe = 497.15 mg g-1), methyl orange (Qe = 247.93 mg g-1), bright blue (Qe = 231.53 mg g-1), and lemon yellow (Qe = 116.77 mg g-1), as well as heavy metal ions like hexavalent chromium (Cr(VI),Qe = 193.17 mg g-1). Further investigations on the adsorption of amaranth red and Cr(VI) indicated that SA-PP achieved equilibrium after 30 min. The process followed the pseudo-second-order and Langmuir isotherm models, indicating monolayer adsorption. The adsorption efficacy of SA-PP remained consistent across a wide range of pH levels. After five cycles, SA-PP was able to retain more than 60 % of its adsorption capacity, highlighting its potential as a sustainable, cost-effective, non-toxic, and biodegradable adsorbent for wastewater treatment applications.

This study introduces Magnetic Starch (MAST), an innovative material designed for the efficient and rapid removal of water contaminants. MAST is synthesized by integrating polyethyleneimine and magnetic Fe3O4 nanoparticles into a starch composite. It exhibits a saturation magnetization of 7.3 emu/g and a functional surface area of 3.55 m² g−1. MAST's amine group density is 12.03 mmol/g, indicating a strong affinity for pollutants. Notably, MAST demonstrates exceptional adsorption capacities for various hazardous substances, including diclofenac sodium (620.51 mg g−1), methyl orange (470.85 mg g−1), amaranth (193.71 mg g−1), and hexavalent chromium (164.62 mg g−1). Thermodynamic studies reveal that the adsorption process is spontaneous and endothermic, with increased efficiency at higher temperatures, indicating suitability across various thermal conditions. MAST achieves rapid equilibrium within 20 minutes, conforms to pseudo-second-order and Langmuir models, and exhibits selective adsorption in complex matrices. These attributes underscore its potential for broad environmental remediation applications. Furthermore, MAST can be easily separated from water using magnets and retains 60% of its effectiveness after five usage cycles, endorsing its feasibility for repeated use.

Aragonite, a metastable crystal form of calcium carbonate, exhibits the highest density and hardness among all species. Diversified forms of aragonite composites can be produced through different preparation processes, forming a relatively complete system in various application fields. Their properties and applications are determined by their multiple levels of structures and shapes. However, research information for aragonite-related fields is currently lacking integration and clarification. This review provides a comprehensive overview on the preparation methods, properties, and potential applications of three main aragonite-based materials: aragonite particles, nacre-inspired mineralized aragonite composites (NMACs), and shell-derived aragonite nanoparticles (SANPs). The formation mechanisms and versatile applications of these materials are thoroughly surveyed based on their initial precursor, reaction conditions, product forms including filler of aragonite whiskers synthesized by carbonation reaction; functional materials of NMACs fabricated by amorphous calcium carbonate-organic matter (ACC-OM) mediated process; biomedical materials of SANPs derived from aragonite cockle shells. Overall, this review aims to provide assistance for customized production of aragonite-based materials as well as new research directions for both pure aragonites and its composites. The preparation methods, properties and potential applications for the three main aragonite-based materials, including the filler of aragonite whiskers synthesized by carbonization reactions, the functional materials of nacre-inspired mineralized aragonite composites (NMACs) produced by biomimetic fabrication process and the medical materials of shell-derived aragonite nanoparticles (SANPs) derived from cockle shells through top-down methods, are summarized. image

Fe-N-C materials have been regarded as one of the potential candidates to replace traditional noble-metal-based electrocatalysts for the oxygen reduction reaction (ORR). It is believed that the structure of carbon support in Fe-N-C materials plays an essential role in highly efficient ORR. However, precisely designing the morphology and surface chemical structure of carbon support remains a challenge. Herein, we present a novel synthetic strategy for the preparation of porous carbon spheres (PCSs) with high specific surface area, well-defined pore structure, tunable morphology and controllable heteroatom doping. The synthesis involves Schiff-based polymerization utilizing octaaminophenyl polyhedral oligomeric silsesquioxane (POSS-NH2) and heteroatom-containing aldehydes, followed by pyrolysis and HF etching. The well-defined pore structure of PCS can provide the confinement field for ferroin and transform into Fe-N-C sites after carbonization. The tunable morphology of PCS can be easily achieved by changing the solvents. The surface chemical structure of PCS can be tailored by utilizing different heteroatom-containing aldehydes. After optimizing the structure of PCS, Fe-N-C loading on N,S-codoped porous carbon sphere (NSPCS-Fe) displays outstanding ORR activity in alkaline solution. This work paves a new path for fabrication of Fe-N-C materials with the desired morphology and well-designed surface chemical structure, demonstrating significant potential for energy-related applications.

Stimulus-responsive multimodal luminescence (MML) within a single material system is highly desirable for anti-counterfeiting and information encryption applications. However, achieving adjustable MML within a unified material framework is challenging due to the distinct responses of different luminescence modes to a common external stimulus. In the work, a novel approach is devised for regulating the aggregation or dispersion state of carbon dots (CDs) to exhibit responsive MML, including fluorescence (FL), room temperature phosphorescence (RTP), and chemiluminescence (CL). Specifically, aggregation-caused luminescence quenching CDs (ACQCDs) are synthesized via a one-step hydrothermal method using levofloxacin. These ACQCDs exhibit ACQ and significant aggregation-induced color change effects. When ACQCDs interact with paper-based materials, they form hydrogen bonds, establishing a high-density hydrogen bond network that induces ACQCDs aggregation. Upon external stimulation, the hydrogen bond network undergoes dynamic changes, triggering ACQCDs dispersion. This process effectively deactivates nonradiative defect centers, stabilizes triplet excitons, and promotes simultaneous MML of tunable FL, RTP, and CL. The integration of multimodal luminescence with external stimulus input enables the creation of a programmable multi-input logic gate, offering significant potential for encoded information anti-counterfeiting applications. Overall, this research provides valuable insights into the conduction of MML CDs, thereby advancing the utilization of nanomaterials in intelligent encryption and anti-counterfeiting technologies. The findings pave the way for the development of more sophisticated and secure anti-counterfeiting measures based on the unique luminescent properties of CDs.

针对富硒养殖业中潜在的硒与抗生素共存废水,以虾壳制备的氮掺杂多孔生物炭(NBC)为骨架,原位合成纳米零价铁(nZVI),构建具有NBC和nZVI活性功能成分的复合材料(NBC-nZVI),采用扫描电镜(SEM)、氮气吸附、X射线衍射(XRD)、傅里叶红外光谱(FTIR)和X射线光电子能谱(XPS)表征样品.考察NBC-nZVI对Se(Ⅳ)和环丙沙星(CIP)的同步吸附行为.结果表明,NBC-nZVI对CIP和Se(Ⅳ)的去除性能优于NBC和nZVI;Se(Ⅳ)优先于CIP被吸附于NBC-nZVI表面,并介导CIP的吸附,提高NBC-nZVI对CIP的去除性能;CIP对NBC-nZVI吸附Se(Ⅳ)无明显影响;双组分体系中NBC-nZVI对CIP和Se(Ⅳ)的吸附行为符合准二级动力学和Sips等温模型,对CIP与Se(Ⅳ)的最大理论吸附量分别为130.6,335.7 mg/g.

In this study, an amine-rich gel (ARAS) was prepared by chemically altering Acacia senegal (AS). ARAS acts as an adsorbent for selenium. Owing to the introduction of amino functional groups and a remarkable specific surface area (91.89 g/m2), ARAS shows maximum adsorption capacities at 75 and 130 mg g(-1) for Se(IV) and Se(VI), respectively. The removal efficiency of ARAS is higher (omega(Se)(IV) = 98.2 % and omega(Se)(VI) = 98.6 %) at lower concentrations (CSe(IV) = 100 ppm and C-Se(VI) = 95 ppm) and the adsorption equilibrium is achieved within 60 min. The adsorption process of Se (IV) and Se (VI) via ARAS is elucidated using the Quasi-Second-Order kinetic and Langmuir models. The enhanced adsorption capacity of the adsorbent could be attributed to the synergistic effects of electrostatic attraction, hydrogen bonding, and specific physicochemical properties. Thermodynamic studies reveal that the surface adsorption process is spontaneous and exothermic. Notably, ARAS maintains remarkable adsorption stability under a variety of solution conditions, including variable pH (4-11), NaCl concentrations (0-1 M), and the presence of organic solvents. It retains approximately 60 % of its initial adsorption capacity for Se(IV) and Se(VI) after three adsorption cycles. Therefore, ARAS with its costeffectiveness and exceptional performance shows considerable potential for applications in water treatment.

Nanoscale zero-valent iron (nZVI) has been widely used in the reductive removal of contaminants from water, yet it still fights against the inherent passive cover and the raise of medium pH. In this study, nZVI was supported onto a nitrogen-doped biochar (NBC) that was prepared by pyrolyzing shrimp shell for efficiently sequestrating aqueous selenite (Se(IV)). The resultant composite (NBC-nZVI) revealed a higher reactivity and electron utili-zation efficiency (EUE) than the bare nZVI in Se(IV) sequestration because of the positive charge, the buffering effect and the good conductivity of NBC. The kinetic rate and EUE of NBC-nZVI were increased by 143.4% and 15.3% compared to the bare nZVI, respectively, at initial pH of 3.0. The high removal capacity of 605.4 mg g-1 for NBC-nZVI was obtained at Se(IV) concentration of 1000 mg L-1, initial pH of 3.0, NBC-nZVI dosage of 1.0 g L-1 and contact time of 12 h. Moreover, NBC-nZVI exhibited a strong tolerance to solution pHs and coexisting compounds (e.g., humic acid) and could reduce the Se(IV) concentration from 5.0 mg L-1 to below the limit of drinking water (50 mu g L-1) in real-world samples. This work exemplified a utilization of shrimp shell-derived NBC to simultaneously enhance the reactivity and EUE of nZVI for reductively removing contaminants.

Pearls are an edible and medicinal resource with whitening activity and nutritional value in China. In the previous study, we found that the pearl shell meat hydrolysate showed dual activities of antioxidation and tyrosinase inhibition, which were similar to the activities of pearls. In this research, a pearl shell meat hydrolysate was isolated, identified and screened by molecular docking, and three peptides FLF, SPSSS and WLL with high tyrosinase inhibitory activities were obtained. The results indicated that FLF, SPSSS and WLL could effectively inhibit tyrosinase activities and the inhibition rates (1.0 mg mL-1) were 54.32%, 65.26% and 57.50%, respectively. The results of a zebrafish whitening experiment showed that the tyrosinase activities of zebrafish treated with FLF, SPSSS and WLL decreased by 75.41%, 62.87% and 64.99% (p < 0.05), respectively, and the melanin content decreased by 37.34%, 38.52% and 40.39% (p < 0.05), respectively. In a B16F10 cell whitening experiment, compared with a control group, FLF, SPSSS and WLL also showed a significant whitening effect, the tyrosinase activities decreased by 84.08%, 79.08% and 77.45% (p < 0.05), respectively, and the melanin content decreased by 42.23%, 34.37% and 34.02% (p < 0.05), respectively. Moreover, the active peptides could act on three signal pathways including Wnt/β-catenin, MAPK and MC1R/α-MSH and significantly downregulated the expressions of the signaling factors WNT4, MITF, β-catenin, ERK, JNK, TRP1 and TRP2 (p < 0.05). The results demonstrated that the whitening active peptides were edible natural antioxidants, tyrosinase inhibitors and skin anti-melanin agents, which could be added to functional foods as food ingredients.

The hydrophobic cuticle seal and the low affinity of disulfide bonds inhibit the performance of human hair (HH), a renewable keratin waste with high sulfur content, toward the remediation of aqueous Hg (II). Herein, the mechanical activation (MA) was used to crack the cuticle seal and expose the disulfide bonds for the reduction by ammonium thioglycolate, preparing a modified HH (MTHH) with high density of thiol groups. With a high distribution coefficient (2.6 x 106 mL/g), such MTHH revealed an uptake capacity over 470 mg/g, capable of decreasing the Hg(II) concentration from 1.0 mg/L to well below 2 lg/L in the real-world samples. Moreover, MTHH with the added benefits of high selectivity and low cost could significantly reduce the phytotoxicity of Hg(II) toward aquatic crops. MTHH diminished the mercury intake of water spinaches from 24.64 to about 0.06 mg/kg when cultivating in the water with a Hg(II) concentration of 4.0 mg/L. Density functional theory (DFT) calculations and characterizations such as X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR) demonstrated that the bonding of Hg(II) to thiol groups was responsible for the Hg(II)-selective and effi-cient capture. This work exemplifies a sustainable, inexpensive and user-friendly platform of keratin waste for the efficient decontamination of aqueous Hg(II).(c) 2022 Elsevier B.V. All rights reserved.

以富硒核桃为原料经提油后得到富硒核桃粕,以血管紧张素转化酶(ACE)抑制作为评估标志,经单因素影响试验和响应面优化富硒核桃粕蛋白ACE抑制肽的制备工艺,并将酶解物进行超滤分离、氨基酸组成分析和硒含量测定.结果显示,富硒核桃粕蛋白ACE抑制肽的最佳酶解温度为35℃,酶解时间为2.52 h,pH值7.5、底物浓度5.39％、加酶量0.33％,在此试验条件下进行的验证试验ACE抑制率为79.13％,与理论值为78.84％较相符.最优酶解物经3 ku超滤膜分离,发现<3 ku超滤组分在1 mg/mL浓度下ACE抑制活性高达77.78％.同时,富硒核桃粕蛋白ACE抑制肽酶解物中疏水性氨基酸占27.19％,硒元素在酶解物和<3 ku超滤组分中的含量分别为0.82 mg/kg和1.35 mg/kg.该研究为食物源性补硒产品和辅助降血压健康食品的研究与开发提供了理论依据.