TiO2纳米管是Ti经阳极氧化及热处理后得到的具有良好生物相容性、高亲水性、无明显细胞毒性的材料.大量学者认为这种材料可用于口腔种植体颈部与软组织接触的位置,并且有研究证明TiO2纳米管可以促进软组织细胞早期粘附、增殖.又因为TiO2纳米管具有装载分子及缓释的作用,有学者在纳米管中装载金属离子、药物、活性分子等物质,希望能够进一步促进软组织封闭,但促进作用有限,需要在特定的时间段及药物浓度下才对软组织粘附、增殖有促进作用,并且纳米管缓释效果又受到纳米管尺寸、装载分子电荷性质等多因素影响.载药TiO2纳米管与软组织封闭之间的关系是很新颖的研究方向,期待能有更多研究着眼于探究如何得到稳定的TiO2纳米管缓释效果.

目的 探讨胶原蛋白类可吸收口腔修复膜修复口腔内软组织浅层缺损的临床效果.方法 共纳入口腔内软组织浅层缺损患者32例.缺损部位为唇部、舌、颊部、腭部、前庭沟及口底.随机将研究对象分配成实验组和对照组各16例进行非劣效性实验.实验组采用胶原蛋白类可吸收口腔修复膜,对照组采用脱细胞基质类口腔修复膜.分别在术后7天,术后1个月和术后3个月记录实验组和对照组的创面愈合率、瘢痕形成率以及有无过敏、排斥、炎症反应.结果 实验组和对照组口腔修复膜在术后都发生吸收降解,创面不断缩小.术后3个月创面愈合完全,留有少量瘢痕.与对照组相比,实验组的创面愈合率较高,瘢痕形成率较低,差异有统计学意义.实验组和对照组都不引起过敏、排斥、炎症反应.结论 胶原蛋白类可吸收口腔修复膜能够促进口腔内软组织浅层缺损更快地修复,并且在修复过程中不引起过敏、排斥、炎症反应,修复后的瘢痕小.

目的 通过对拔除的双侧下颌第三磨牙的拔牙窝进行不同处理,比较两侧第二磨牙远中牙周的恢复情况,以指导临床预防术后并发症.方法 选取2021年1—9月中国中医科学院眼科医院口腔科门诊拔牙患者中符合纳入标准的拔除双侧下颌第三磨牙患者12例,年龄≥26岁,患牙为中、低位阻生,近中或水平方向,且双侧患牙阻生程度相似.随机选择一侧作为实验组,在患牙拔除即刻将骨替代品和自体血制品浓缩生长子混合后填塞拔牙窝;另一侧为对照组,拔牙窝不做特殊处理.分别测量并记录拔牙前、术后即刻及术后6个月时两侧第二磨牙远中(包括第二磨牙颊侧远中、远中和舌侧远中3个位点)的牙周探诊深度及附着丧失,比较分析两组牙周恢复情况.结果 术后即刻两组附着丧失程度比较差异无显著性(P>0.05),而术后6个月对照组附着丧失程度高于实验组,差异有显著性(P<0.05).根尖片提示,实验组术后6个月第二磨牙远中的牙槽骨高度基本恢复至正常水平,其恢复情况明显好于对照组.结论 患者26岁以后拔除下颌中、低位阻生第三磨牙时,第二磨牙远中牙周恢复不佳,存在不同程度的牙周问题,牙周探诊深度及附着丧失均有明显增加,将骨替代品和自体血制品浓缩生长因子混合后填塞拔牙窝,可促进邻近第二磨牙远中的牙周愈合.

目的 通过临床观察评估下颌中、低位阻生第三磨牙拔除后相邻第二磨牙远中的牙周恢复状况及牙周恢复是否与患者拔牙时的年龄存在一定的关系.方法 收集2021年1—9月在中国中医科学院眼科医院口腔科门诊拔除患牙中符合纳入标准的63例患者的63颗下颌阻生第三磨牙纳入研究,患牙为中、低位阻生,近中或水平方向,将研究对象分成≥26岁年龄组(42例)及<26岁年龄组(21例),分别测量并记录下颌阻生第三磨牙拔除术前及术后6个月时第二磨牙远中的牙周探诊深度及附着丧失,测量包括3个位点即第二磨牙颊侧远中、远中及舌侧远中,然后统计数据进行分析比较,评估第二磨牙远中牙周的恢复情况及其与患者年龄的关系.结果 ≥26岁年龄组患者下颌中、低位阻生第三磨牙拔除6个月后相邻第二磨牙远中存在明显的附着丧失,与<26岁年龄组患者比较,附着丧失明显增大,两个年龄组附着丧失的差异有显著性.结论 26岁以后拔除下颌中、低位阻生第三磨牙时,第二磨牙远中牙周恢复不佳,存在不同程度的牙周缺损;年龄是影响下颌中、低位阻生第三磨牙拔除后第二磨牙远中牙周恢复的一个重要因素,早期拔除患牙有利于第二磨牙远中恢复.

少数牙间隔缺失的修复有一定难度,固定义齿修复作为传统的修复方式之一,在临床中仍有其存在的价值,特别是对少数牙间隔缺失的病例.我院修复科于2009年诊治左上牙间隔缺失1例,并行烤瓷复合固定桥(含栓体栓道)修复,经10年随访观察,无明显异常,患者自觉美观、功能良好.

在牙齿的发育期间,由于压力使两个牙胚发生完全或者不完全融合,形成融合牙.融合牙在乳牙和恒牙均可发生,同时也可以发生在正常牙与多生牙之间.本文报道左上颌恒中切牙与近中多生牙融合并发生慢性根尖囊肿1例,就其口腔检查、治疗过程、愈合及讨论进行阐述,使临床医生对本病有进一步的认识.

The soft tissue sealing at the transmucal portion of implants is vital for the long-term stability of implants. Hydrogenated titanium nanotubes (H2-TNTs) as implant surface treatments were proved to promote the adhesion of human gingival fibroblasts (HGFs) and have broad usage as drug delivery systems. Bovine serum albumin (BSA) as the most abundant albumin in body fluid was crucial for cell adhesion and was demonstrated as a normal loading protein. As the first protein arriving on the surface of the implant, albumin plays an important role in initial adhesion of soft tissue cells, it is also a common carrier, transferring and loading different endogenous and exogenous substances, ions, drugs, and other small molecules. The aim of the present work was to investigate whether BSA-loaded H2-TNTs could promote the early adhesion of HGFs; H2-TNTs were obtained by hydrogenated anodized titanium dioxide nanotubes (TNTs) in thermal treatment, and BSA was loaded in the nanotubes by vacuum drying; our results showed that the superhydrophilicity of H2-TNTs is conducive to the loading of BSA. In both hydrogenated titanium nanotubes and non-hydrogenated titanium nanotubes, a high rate of release was observed over the first hour, followed by a period of slow and sustained release; however, BSA-loading inhibits the early adhesion of human gingival fibroblasts, and H2-TNTs has the best promoting effect on cell adhesion. With the release of BSA after 4 h, the inhibitory effect of BSA on cell adhesion was weakened.

Oral and maxillofacial space infections (OMSI) are common diseases of the facial region involving fascial spaces. Recently, OMSI shows trends of multi drug-resistance, severe symptoms, and increased mortality. OMSI treatment principles need to be updated to improve the cure rate. Based on the clinical experiences of Chinese experts and with the incorporation of international counterparts' expertise, the principles of preoperative checklist, interpretation of examination results, empirical medication principles, surgical treatment principles, postoperative drainage principles, prevention strategies of wisdom teeth pericoronitis-related OMSI, blood glucose management, physiotherapy principles, Ludwig's angina treatment and perioperative care were systematically summarized and an expert consensus on the diagnosis and treatment of OMSI was reached. The consensus aims to provide criteria for the diagnosis and treatment of OMSI in China so as to improve the level of OMSI treatment.

Background The purpose of this study was to investigate the association between IL-1A (-889C/T, rs1800587) polymorphism and susceptibility of chronic periodontitis. Methods A systematic literature search was carried out in the databases updated on July 1, 2019, including PubMed, Embase, Cochrane Library and Web of Science. Through STATA 14.0 software, the association between IL-1A (-889C/T) polymorphism and susceptibility of chronic periodontitis was calculated by pooled odds rations (ORs) and 95% confidence intervals (CIs). Harbord test was used for the publication bias. Results The results of overall meta-analysis revealed that IL-1A (-889C/T) polymorphism was associated with the susceptibility of chronic periodontitis among all the genetic models, including allele contrast (T vs. C, OR (95% CI): 1.297 (1.038-1.622), P=0.022), dominant model (TT+CT vs. CC, OR (95% CI): 1.337 (1.015-1.761), P=0.039), recessive model (TT vs. CC+CT, OR (95% CI): 1.453 (1.138-1.856), P=0.003), and codominant model (TT vs. CC, OR (95% CI): 1.555 (1.187-2.038), P=0.001; CT vs. CC, OR (95% CI): 2.559 (1.245-5.260), P=0.011). The results of subgroup analyses indicated that IL-1A (-889C/T) polymorphism was closely related to the susceptibility of chronic periodontitis in African population (T vs. C, OR (95% CI): 1.277 (1.039-1.571), P=0.020; TT+CT vs. CC, OR (95% CI): 1.357 (1.061-1.735), P=0.015; TT vs. CC, OR (95% CI): 1.599 (1.115-2.292), P=0.011), in European population (TT vs. CC+CT, OR (95% CI): 1.645 (1.112-2.435), P=0.013; TT vs. CC, OR (95% CI): 1.639 (1.044-2.574), P=0.032) and in American population (CT vs. CC, OR (95% CI): 6.404 (3.000-13.669), P<0.001). Conclusions IL-1A (-889C/T) polymorphism is associated with the susceptibility of chronic periodontitis in African, European and American populations according to the currently available evidence. However, more large-scale, multi-ethnic case-control studies are required to be conducted in future to confirm the role of IL-1A (-889C/T) gene in the occurrence and development of chronic periodontitis.

目的:探讨不同纤维桩表面处理方式对牙根修复后抗折裂强度的影响.方法:收集行正畸拔除的前磨牙120颗作为本研究样本,随机将120个纤维桩分为对照组、喷砂组和过氧化氢酸蚀组,每组40例.对照组纤维桩表明不给予任何处理,喷砂组给予氧化铝砂粒持续喷砂粗化处理,过氧化氢酸蚀组给予10％过氧化氢溶液处理,均包埋于纤维桩道预备好的离体牙内,采用相同树脂制备成核,行全冠修复与黏固,再模拟口腔内部环境给予样本牙冷热循环处理,经相同环境加载后,置于电子万能实验机获取样本牙抗折裂强度数据.对比三组离体牙样本体型数据、离体牙断裂方式、抗折裂强度,对三组进行为期24个月的定期随访,统计三组修复体断裂率,并采用Kaplan-Meier曲线和Log Rank法分析三组的生存状况.结果:三组的离体牙样本关于牙齿长度、牙根长度、颈部颊舌径及颈部近远中径对比差异无统计学意义(P＞0.05);喷砂组和过氧化氢酸蚀组的离体牙抗折裂强度显著强于对照组(P＜0.05);喷砂组和过氧化氢酸蚀组的牙齿折裂总发生率分别为20.00％和22.50％,均显著低于对照组的70.00％(P＜0.05).但两组间比较差异无统计学意义(P＞0.05).喷砂组、过氧化氢酸蚀组的生存状况显著优于对照组.结论:前磨牙修复过程中,对纤维桩表面进行喷砂或过氧化氢酸蚀处理均能提高牙根修复后抗折裂强度,改善修复体修复效果生存状况,且两种纤维桩表面处理方式对离体牙样本的断裂方式和抗折裂强度影响相当.

The main aim of this study was to develop a 3D printed stem cell-responsive polyethylene glycol diacrylate (PEGDA) and sodium alginate (SA) composite hydrogel system (PEGDA/SA) loaded with basic fibroblast growth factor (bFGF) suitable for dental pulp tissue engineering. The PEGDA/SA loaded with bFGF (PEGDA/SA-bFGF) at different concentration ratios were 3D printed via stereolithography (SLA) under optimal processing conditions, followed by ionic crosslinking. The morphological and porous structure of the 3D printed PEGDA/SA-bFGF constructs and release profile of the bFGF were analyzed. The cellular compatibility of the hydrogel constructs was evaluated using human dental pulp stem cells (hDPSCs)in vitro. Subsequently, biocompatibility and tissue regenerative potential of the hydrogel constructs were evaluated in immunodeficient mice model. The in vitro cell culture results of PEGDA/SA-bFGF constructs exhibited high cell compatibility and supported cell proliferation, irrespective of their concentration ratios. Thein vivo animal experiment results showed that the hDPSCs-laden PEGDA/SA (PEGDA/SA-hDPSCs) group failed to form a good pulp structure even at various concentration ratios. When the mass ratio of PEGDA/SA was 25:1, hDPSCs-laden PEGDA/SA-bFGF (PEGDA/SA-bFGF-hDPSCs) group also failed to form dental pulp-like tissue but a partial loose connective tissue formation was observed. However, when the mass ratio of PEGDA/SA reduces to 20:1 ~ 15:1, the PEGDA/SA-bFGF-hDPSCs group tends to form a well-organized pulp structure post 4 weeks implantation, thus highlighting the potential of this particular hydrogel scaffolding system for dental pulp tissue regenerative applications.

Objective: To assess the effectiveness of osseointegrated implant supported prostheses in the rehabilitation of severe orbital deformity. Methods: Retrospective case series. The study collected 6 patients (6 eyes) with severe orbital deformity, who were treated with osseointegrated implant supported prostheses between 2010 and 2016 in Beijing Tongren Eye Center, Beijing Tongren Hospital. Data included demographic characteristics, causes of the deformity, the history of radiotherapy, the site, number and survival of implants, and the ability to wear prostheses. Results: Among the 6 patients, 4 were males, and 2 were females, with a mean age of 27 (16-44) years. The deformity resulted from evisceration or enucleation and radiotherapy for malignancies in 4 patients, from evisceration because of inflammatory pseudotumor in 1 patient, and from enucleation and debridement because of explosion injury and secondary infection in 1 patient. Each patient received 3 implants at the first operation. A total of 18 implants were installed, including 9 placed into the lateral aspect of the supraorbital rim, 6 into the lateral aspect of the infraorbital rim, 1 into the medial aspect of the supraorbital rim, and 2 into the medial aspect of the infraorbital rim. One superior lateral implant failed half a year after implantation, and an additional implant was implanted into the medial aspect of the inferior medial orbital rim for prostheses retention. All the patients were followed up for more than 2 years. No other failures were observed. The soft tissue reaction was acceptable in all patients. All of them were able to wear prostheses with satisfying appearance. Conclusions: Osseointegrated implants provid excellent retention for orbital prostheses. This technique could be used in patients with severe orbital deformity to improve their life quality. (Chin J Ophthalmol, 2019, 55: 665-669).

目的 评价微创拔除上颌前部埋伏阻生牙的临床效果.方法 上颌前部单侧完全骨埋伏阻生牙患者11例(男5例,女6例),所有患者拔牙术前均拍摄锥形束CT精确定位阻生牙,拔牙术中使用超声骨刀、微创牙挺等微创器械去除骨阻力、分割牙齿、增隙,比较患者手术前及手术中血压的变化.拔牙术后第1天、第3天以视觉模拟评分法(VAS)评价患者术后疼痛情况,拔牙术后第3天评价患者术区肿胀情况,拔牙术后第7天评价患者伤口愈合情况.结果 本组患者阻生牙包括水平阻生6例、垂直阻生3例、倒置阻生2例,所有病例阻生牙拔除术均在局部麻醉下顺利完成,拔牙术前及拔牙术中血压波动不明显,患者收缩压与舒张压变化差异无统计学意义(P>0.05).术后患者均按时复诊,术后疼痛、肿胀均不明显,拔牙术后第1天及第3天患者疼痛VAS评分均为5分以下(0-5分),拔牙术后第3天术区轻度肿胀者占比72.7％、中度肿胀者占比18.2％.术后第7天所有患者伤口无感染,愈合良好.结论 微创拔除上颌前部埋伏阻生牙,创伤轻微,术后反应小,精准的手术设计是成功的关键,超声骨刀在去骨、分牙中优势明显,值得临床推广.

目的 比较左旋咪唑联合VB12对复发性口腔溃疡患者的治疗效果及其血清IL-6、VB12水平的影响,探讨治疗复发性口腔溃疡更好的治疗方法.方法 选择2015年9月～2016年9月在我院口腔黏膜科就诊的128例复发性口腔溃疡患者作为研究对象.按照治疗方式不同分为联合治疗组(左旋咪唑联合VB12治疗)和左旋咪唑组(单独采用左旋咪唑),每组64例.治疗1月后,观察口腔溃疡的愈合情况,同时采用疼痛视觉模拟量表(VAS)评分评估患者的疼痛情况;检测所有患者血清中的IL-6、VB12水平.结果 整个试验随访率93.75％.联合治疗组VAS评分小于左旋咪唑组,差异有统计学意义(P＜0.05);联合治疗组口腔溃疡痊愈率大于左旋咪唑组,差异有统计学意义(P＜0.05);联合治疗组血清中VB12水平大于左旋咪唑组,差异有统计学意义(P＜0.05);联合治疗组血清中IL-6水平和左旋咪唑组相比差异无统计学意义(P＞0.05).结论 左旋咪唑联合VB12对于复发性口腔溃疡治疗效果更佳,其可能是通过提高血清中VB12水平来发挥作用的.因此,对于临床上治疗效果欠佳的复发性口腔溃疡患者,可以选用左旋咪唑联合VB12进行治疗.

Objective To evaluate the association between tooth surface loss and gastroesophageal reflux disease (GERD). Methods The dental impression was obtained from 15 patients with GERD and 15 controls at baseline and six months.The tooth surfaces of these replicas were digitized using an optical scanner. The depth of tooth surface loss was recorded after matching the digitized model at baseline and six months. The association between GERD and the depth of tooth surface loss was evaluated. Results One patient was lost to follow-up and the other 29 participants(580 teeth) were included. The tooth surface loss in patients with GERD(12.07 ± 1.85 μm) was more than that in the control(3.23 ± 0. 48 μm). The tooth surface loss was associated with GERD (t=3.193). The tooth tissue loss on palatal and occlusal surface was more than that on buccal surface in patients with GERD. Conclusion The tooth surface loss in patients with GERD was greater than that in the healthy controls.

Marginal gap (MG) was the most important factor to evaluate the success of crowns. The study was to assess the MG of lithium disilicate glass ceramic crowns with spectral domain optical coherence tomography (OCT) and stereomicroscopy in vitro, and to provide evidence to measure the MG with OCT. Consistency was observed between OCT and stereomicroscopy to measure the MG after cementation. There was no significant difference between the MG of lithium disilicate glass ceramic crowns using OCT and stereomicroscopy (OCT 59.55 +/- 7.22 mu m, stereomicroscope 59.48 +/- 6.53 mu m, P = 0.736) after cementation. OCT was a noninvasive diagnostic technique to measure the MG of lithium disilicate glass ceramic crowns. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)

Objective To investigate the effect of coenzyme Q10 on the treatment of periodontitis with coronary heart disease,and analyze its effect on serum TNF-α,IL-6.Methods A total of 84 cases of patients with periodontitis and coronary heart disease were selected,which were treated in hospital from September 2014 to September 2016,and were divided into observation group (42 cases) and control group (42 cases).The patients of control group were treated with periodontal basis therapy.On the basis of control group,the patients of observation group were treated with coenzyme Q10.The patients of two groups were all treated for 2 weeks in a row.The clinical effect,safety and changes of periodontal index,inflammatory mediators (TNF-α,IL-6,IL-8 and IL-1β) of two groups were observed and compared.Results After the appropriate treatment,the patients of the observation group got a total effective rate of 76.2％,which was significantly better than the 54.8％ of the control group (P＜0.05);Periodontal index of the two groups were all significantly decreased than before (P＜0.05),and the patients of the observation group got more significant decline (P＜0.05);Serum inflammatory mediators (TNF-α,IL-6,IL-8 and IL-1β) levels of the two groups were all significantly decreased than before (P＜0.05),and the patients of the observation group got more significant decline (P＜ 0.05);There were no obvious adverse reactions occurred between the two groups.Conclusion To treat the patients with periodontitis and coronary heart disease,coenzyme Q10 can get some good clinical effects,significantly improve the patient's periodontal inflammation,reduce serum inflammatory mediators levels,which makes it have an important clinical significance.

OBJECTIVE:This study aimed to investigate the potential effects of the combination therapy of irsogladine maleate (IM) and azithromycin (AZM) on the inflammation in lipopolysaccharide (LPS)-induced gingival epithelial cells. METHODS:Human gingival epithelial cell OBA-9 was stimulated by LPS to construct the periodontitis model, followed by the treatment of irsogladine maleate (IM) or azithromycin (AZM) with different concentration. Transepithelial electrical resistance (TER) of cells in each group was analyzed, and qRT-PCR and western blotting were used to detect the expressions of inflammatory cytokines. Immunofluorescence staining was performed to detect the protein expression. RESULTS:The TER for cells was significantly decreased while the inflammatory cytokines expressions including IL-6, IL-8, IL-1β and TNF-α were all significantly increased by LPS compared to the control (P<0.05). However, TER was increased significantly, whereas the cytokine levels were decreased by IM or AZM, but these effects was more apparent in cells treated with IM and AZM combination (P<0.01). Moreover, E-cadherin and vimentin expressions were more positive in the IM and AZM group than in the other groups. The application of ERK and P38 MAPK inhibitors reversed the effects of LPS on cell inflammatory cytokine production and cell TER. CONCLUSION:This study revealed that the combination therapy of IM and AZM performed excellent effects on preventing the inflammatory progression of periodontitis.

Objective:Gastroesophageal reflux disease (GERD) may cause dental erosion.The purpose of this study was to evaluate the impact of GERD,finish line location and marginal adaptation of all ceramic crowns among patients with GERD.In addition,we evaluate the possible association of finish line location on the marginal adaptation of crowns among patients with GERD.Methods:Study participants (N=123) were recruited from the Beijing Tong Ren Hospital of Capital Medical University from May 2014 to May 2015 and had single unit ceramic crown restoration.Medical and dental histories were collected by questionnaire.The distribution and degree of marginal adaptation of crowns were evaluated with clinical criteria for the evaluation of direct and indirect restorations.The association between marginal adaptation and covariates including GERD and finish line location (subgingival/supragingival) was evaluated using generalized linear mixed model.Results:Among the 123 participants enrolled in the study,62 GERD participants had 164 crowns,and 61 healthy participants had 170 crowns.In the multilevel analysis,the marginal adaptation was more likely obtained in healthy participants in comparison with participants with GERD (adjusted odds ratio [aOR]=2.606.P=0.009).In addition,we found supragingival (aOR=3.212.P=0.001) finish line were more likely to result marginal adaptation in comparison with subgingival finish line.Conclusions:The marginal adaptation was more likely obtained in healthy participants in comparison with participants with GERD.Supragingival finishing line were more appropriate for patients with GERD(Beijing Tongren Hospital,2014BJSZR-10).

Objective To investigate the clinical manifestation and surgical approach of supraorbital rim fractures.Methods Seven supraorbital rim fracture patients from 2007 to 2014 were retrospectively reviewed.All patients received CT scan and three cases received HESS charting examinations.Ebonation and/or fractured bone reduction and rigid fixation were performed on all seven patients.The patients were followed up for six months after surgery.Results Diplopia was the chief complain in three patients and the other four patients complained eye discomfort.CT scan showed supraorbital rim fractures in seven cases including four case of communicated fractures and superior rectus compression.Three cases of HESS charting examination indicated extraocular muscle movement restriction.The symptoms improved after surgery in all patients.Conclusion Eye movement restriction due to superior rectus compression is the main symptom of supraorbital rim fractures.The key point of the surgery is superior rectus decompression and orbital rim reconstruction.