目的 探讨治疗前肿瘤标志物联合临床因素在预测直肠癌新辅助放化疗(nCRT)敏感性的价值.方法 收集了2013年8月至2020年12月行nCRT的97例直肠癌患者资料,纳入了性别、年龄、临床T/N分期、距肛缘距离、肿瘤最长径、肿瘤环周比、同步化疗方案、手术间隔时间,以及治疗前的血清CEA、CA199、CA125和CA724.依据肿瘤退缩反应程度进行分组.采用Pearson卡方检验比较组间的基线资料,再将P<0.05的变量纳入多因素模型进行Logistic回归分析.结果 多因素分析结果显示,治疗前CEA≤5 ng/ml和肿瘤最长径≤5 cm是患者出现肿瘤消退明显和pCR的独立预测因子(P<0.05).结论 治疗前血清CEA和肿瘤大小对直肠癌新辅助放化疗敏感性的预测有重要价值.

Objective:This study aims to investigate the efficacy and safety of the CT-guided three-dimensional brachytherapy combined with threedimensional conformal radiotherapy in patients with locally middleand late-stage cervical cancer.Methods:Ninety-seven patients with stage ⅡA-Ⅳ locally middle-and late-stage cervical squamous-cell carcinoma were treated with CT-guided three-dimensional brachytherapy combined with three-dimensional conformal radiotherapy.The short-term response was observed.The survival analysis was conducted by Kaplan-Meier method,and the univariate analysis of prognosis was performed by using log-rank test.The high-risk clinical target volume (HR-CTV) D90,D2cc of bladder,rectum and sigmoid colon and equivalent dose in 2 Gy per fraction (EQD2) were calculated.Results:The overall 2-year survival rate and 2-year progression-free survival rate of 97 patients were 93.8％ and 92.1％,respectively.The differentiation degree and tumor size were significantly correlated with the overall survival (both P ＜ 0.05).According to Radiation Therapy Oncology Group (RTOG) acute and late radiation reaction evaluation criterion,rates of grades 1 and 2 acute lower digestive adverse reaction were 15.5％ (15/97) and 12.4％ (12/97),respectively.The acute gastrointestinal adverse reaction grading was associated with HR-CTV D90 EQD2 (P =0.027).The rate of grades 3 and 4 late digestive system adverse reactions was 1.0％ (1/97).Conclusion:CT-guided three-dimensional brachytherapy combined with three-dimensional conformal radiotherapy in the treatment of locally middle-and late-stage cervical cancer has good efficacy,and the rate of late severe adverse reaction is low.

目的 食管癌是我国常见恶性肿瘤,临床工作中不少老年患者不能耐受手术和化疗而选择单纯放疗作为其根治方式.本研究通过观察老年食管癌患者放疗的毒副作用和长期疗效,探讨高血压或糖尿病对放射性食管和肺损伤的影响,分析其预后影响因素.方法 收集2008-01-04-2017-11-30苏州大学附属第一医院放疗科、江阴市人民医院放疗中心、泰州市人民医院肿瘤科和常州市第二人民医院放疗科4个中心收治的接受单纯放疗≥70岁食管鳞癌患者359例,其中伴2型糖尿病患者59例,高血压147例.分析糖尿病或高血压与放射性食管和肺损伤的关系,Kaplan-Meier法计算生存率(overall survival,OS),Log-rank法行预后单因素分析和Cox法行预后多因素分析.结果 糖尿病和无糖尿病患者≥3级放射性食管炎和放射性肺炎的发生率分别为15.3％和5.7％、16.9％和1.7％,差异均有统计学意义,χ2值分别为6.746和28.761,均P<0.05.高血压和无高血压患者≥3级放射性食管炎和放射性肺炎发生率分别为10.9％和4.7％、8.2％和1.4％,差异均有统计学意义,χ2值分别为4.915和9.873,均P<0.05.全组患者完全缓解率(complete response,CR)和部分缓解率(partial remission,PR)分别为63.0％和32.6％,总有效率(CR+PR)为95.6％.全组1、3、5年生存率分别为76.7％、42.1％和25.3％,中位生存时间26.9个月(95％CI:22.2～31.7个月).选择性淋巴结照射野(elective node irradiation,ENI)和累及野(involved field irradiation,IFI)照射总复发率、野外复发率分别为18.07％、1.20％和16.66％、3.26％,两组差异均无统计学意义,χ2值分别为0.089和0.991,均P>0.05.单因素分析显示,影响老年食管癌三维技术放疗预后的因素有肿瘤T分期、N分期、TNM分期、PS评分、近期疗效、肿瘤部位和放疗剂量.Cox多因素分析显示,TNM分期、近期疗效、PS评分和肿瘤部位是影响生存期的独立预后因素.结论 老年食管癌伴糖尿病或高血压为患者发生≥3级放射性食管和肺损伤的易感因素.老年患者进行IFI放疗不影响预后,TNM分期、近期疗效、PS评分和肿瘤部位是影响生存期的独立预后因素.

Objective To observe the long-term prognosis and analyze the predictive factors of esophageal cancer patients treated with three-dimensional radiotherapy.Methods A total of 373 patients with esophageal squamous carcinoma who received three-dimensional radiotherapy were retrospectively enrolled in this study.Among these,231 cases received three dimensional conformal radiotherapy (3D-CRT) and the other 142 received intensity modulated radiotherapy (IMRT);202 cases received radiotherapy alone,and the other 171 received chemoradiotherapy;249 cases received involved-field irradiation(IFI),and the other 124 received elective nodal irradiation(ENI);60 cases received a total radiation dose of 50-60 Gy,and the other 313 received 60-70 Gy.Kaplan-Meier method was used to calculate the overall survival (OS) and progression-free survival (PFS).The Logrank single factor analysis and Cox multivariate analysis were used to evaluate predictive factors of PFS and OS.Results The 1-,3-,5-year OS and PFS were 69.4％,33.7％,22.9％ and 63.8％,32.8％,22.4％,respectively.The median OS and PFS were 22.7 months (95％ CI 18.6-25.4 months) and 19.2 months (95％ CI 16.7-21.3 months) respectively.Univariate analysis showed that age,gender,tumor location,three-dimensional technology (3D-CRT vs.IMRT),chemotherapy,prophylactic irradiation to lymphatic drainage area and irradiation dose did not influence OS and PFS (P ＞ 0.05).T-stage,N-stage,TNM-stage and gross tumor volume (GTV) were significantly correlated to OS and PFS (x2 =5.836-14.526,P ＜ 0.05).The multivariate analysis showed that N-stage and GTV were independent predictive factors of OS and PFS (x2 =5.345-12.216,P ＜0.05).The OS and PFS of patients with two fields of lymph node metastases were worse than those with only one lymph node field metastasis (x2 =4.467,4.169,P ＜ 0.05).Conclusions The long-term efficacy for esophageal cancer patients could be significantly improved through 3D-CRT technology.N-stage and tumor volume were independent prognostic factors of OS and DFS.The number of lymph node metastasis field is significantly related to prognosis.

Objective To compare the clinical effects, side effects and cosmetic effects of two radiotherapy techniques sequential boost radiotherapy and simultaneous integrated boost radiotherapy after breast conserving surgery for early breast cancer. Methods From January 2014 to May 2017 in Jiangyin People's Hospital, 96 patients with early breast cancer after breast conserving surgery were included in this study. The patients were randomly divided into sequential boost radiotherapy group (49 cases) and simultaneous integrated boost radiotherapy group (47 cases). In sequential boost radiotherapy group, 49 patients received 25 fractions of 2.0 Gy for the total breast during 5 weeks following 5～8 fractions of 2.0 Gy for local tumor bed in 1～1.5 weeks. In simultaneous integrated boost radiotherapy group, 47 patients received 25 fractions of 2.0 Gy for the total breast and 2.4 Gy for local tumor bed during 5 weeks. We investigated the side effects, cosmetic effects and long-term survival. Results The incidence rates of acute skin reaction, late skin reaction, bone marrow suppression and radiation pneumonia were not statistically significant between the sequential boost radiotherapy group and simultaneous integrated boost radiotherapy group (P＞0.05). No significant differences were observed in terms of cosmetic effect between the sequential boost radiotherapy group and simultaneous integrated boost radiotherapy group before, after and 1 year after radiotherapy (P＞0.05). Patients were followed up for 12 to 50 months. The median follow-up time was 29 months and the follow-up rate was 100%. All patients were healthy and survived during the follow-up period. Conclusion The efficacy of sequential boost radiotherapy was similar to that of simultaneous integrated boost radiotherapy after breast conserving surgery for early breast cancer. The simultaneous integrated boost radiotherapy is acceptable with similar cosmetic effect from that sequential boost radiotherapy for early patients after breast conserving surgery, and the treatment time and cost can be significantly reduced.

Objective To compare the prognostic factors and adverse effects of elderly patients with esophageal cancer between radiotherapy alone and concurrent chemoradiotherapy. Methods A total of 479 patients with esophageal squamous cell carcinoma aged 70 years or older were analyzed retrospectively at our institute, from January 2008 to December 2017. The patients were divided into radiotherapy alone group ( 359 cases ) and concurrent chemoradiotherapy group ( 120 cases ) . After Propensity Score Matching ( PSM) , data from matched patients with 102 cases in each group was analyzed. The overall survival (OS) rates, the prognostic factors and adverse effects were assessed. Results The 1, 3, 5-year of OS in radiotherapy alone group after PSM were 77. 4％, 40. 1％, 22. 7％, respectively, and median overall survival time (mOS) was 26. 9 months (95％ CI:18. 7 - 35. 2 months). The chemoradiotherapy group after PSM were 79. 5％, 47. 6％, 35. 7％ and 35. 6 months (95％ CI:23. 2-48.0 months), respectively, while there was no significant difference between the groups (P >0. 05). Subgroup analysis showed that the 1, 3, 5-year of OS and mOS of the patients aged 70 -75 years in radiotherapy alone group were 79. 4％, 41. 0％, 26. 2％ and 29. 2 months, respectively. The patients aged 70-75 years in chemoradiotherapy group were 86. 5％, 56. 1％, 47. 6％ and 48. 9 months, respectively. There was statistically significance between the groups after PSM(χ2 =4. 746, P<0. 05). The univariate prognostic analysis showed that the age, T stage, N stage, clinical stage, short-term efficacy and performance status were influencing factors for OS (χ2 =6. 714-42. 900, P<0. 05). The clinical stage and short-term efficacy were independent prognostic factors for OS (χ2 =5. 007 -9. 181, P<0. 05). In addition , the risk of non-tumor related death of the patients aged 75 years or older in the chemoradiotherapy group was higher than those in the radiotherapy alone group(χ2 =5. 630, P<0. 05). The prevalence of toxicities (≥grade 3) including bone marrow suppression, radiation esophagitis and radiation pneumonia in the chemoradiotherapy group were higher than that in the radiotherapy alone group (χ2 =4. 701 -28. 318, P<0. 05). Conclusions Concurrent chemoradiotherapy, compared with radiotherapy alone, may improve the prognosis of patients aged 70-75 years with esophageal squamous cell carcinoma.

Objective To assess the effects of bladder filling and emptying state on the dose distribution to normal tissue in intracavitary brachytherapy of cervical cancer.Methods We searched databases including PubMed,EMBASE,Cochrane Library,Ongoing Controlled Trial,Chinese Biomedical Literature Database,Chinese Journal FullText Database and Chinese Scientific Journals FullText Database.Quality assessment and data extract was performed for clinical research that met qualifying criteria,then we performed Meta-analysis by StatAl 2.0 software.Results Six studies were involved and 135 patients were included.The Meta-analysis showed that the median dose D50 to the bladder in filling state was significantly lower than in emptying state (SMD=-2.48,95％ CI:-4.37--0.59,P=0.000).D1cm3 and D2cm3 showed no significant difference (P=0.000,0.000).D2cm3 to the rectum in filling state was greater than in emptying state (SMD=0.39,95％ CI:0.06-0.72,P=0.257).D50 and D1cm3 showed no significant difference (P=0.105,0.005).D2cm3 to the sigmoid colon in filling state was greater than in emptying state (SMD=0.81,95％ CI:0.50-1.12,P=0.648).D50 and D1cm3 showed no significant difference (P=0.039,0.000).D1cm3and D2cm3 to the small bowel in filling state was lower than in emptying state (SMD=-3.28,95％ CI:-5.61--0.95,P=0.000 and SMD=-2.98,95％ CI:-4.68--1.28,P=0.000).D50 showed no significant difference (P=0.008).When sensitivity analyses were performed by the sequential dropping of a single study,differences were observed when the study of Patra et al was excluded.Conclusions In intracavitary brachytherapy of cervical cancer,the dose distribution to the bladder and small bowel in filling bladder state were lower than those in emptying bladder state,the results of sigmoid colon and rectum were just opposite.Confined by article quality and quantity,further evaluations in adequately powered large control trail are needed to confirm these findings.

Objective:To investigate the efficacy and toxicity of radiotherapy alone,concurrent radiochemotherapy and radiotherapy combined with adjuvant chemotherapy for patients with cervical carcinoma.Methods:Total of 127 patients with cervical cancer receiving radiotherapy in the First Affiliated Hospital of Soochow University between October 2008 and October 2013 were enrolled into this study and divided into three groups:radiotherapy alone group (n =45),radiotherapy with concurrent cisplatin chemotherapy group (n =58) and radiotherapy combined with adjuvant chemotherapy (paclitaxel plus carboplatin) group (n =24).All patients in the three treatment groups received radical radiotherapy including pelvic external radiotherapy and 192Ir brachytherapy.The efficacy,toxicities and survival of three groups were observed.Results:All patients finished treatment.The median follow-up time was 41 months.The rates of complete response of radiotherapy alone group,radiotherapy with concurrent cisplatin chemotherapy group and radiotherapy combined with adjuvant chemotherapy group were 53.3％ (24/45),77.6％ (45/58) and 62.5％ (1 5/24),respectively (P =0.496);the 2-year overall survival rates of these three treatment groups were 66.2％,85.4％ and 74.9％,respectively (P ＜ 0.05);the 3-year overall survival rates were 64.2％,82.4％ and 74.9％,respectively (P ＜ 0.05);the 5-year overall survival rates were 54.8％,78.1％ and 74.9％,respectively (P ＜ 0.05);the 2-year progression-free survival rates were 57.9％,87.2％ and 69.3％,respectively (P ＜ 0.05);the 3-year progression-free survival rates were 56.1％,83.8％ and 69.3％,respectively (P ＜ 0.05).The toxicities were mainly in grades 1-2.There were no significant differences in the rates of leucopenia,thrombocytopenia,nausea and vomiting and bladder injury among the three treatment groups (all P ＞ 0.05).Conclusion:The efficacy of radiotherapy with concurrent cisplatin chemotherapy regimen for patients with cervical cancer is better than radiotherapy alone or radiotherapy combined with adjuvant chemotherapy.This concurrent radiochemotherapy regimen can significantly improve the overall survival and progression-free survival of patients with cervical cancer,but the rates of side effects may be increased.For the cervical cancer patients who can not tolerate concurrent radiochemotherapy,the adjuvant chemotherapy can achieve better benefit in survival rather than radiotherapy alone.

Objective To study the dose distribution of five clinical technologies commonly used in simulation phantom of breast cancer after radical mastectomy are observed and analyzed by using MOSFET detector.The dose validation from TPS plan is tested for clinical treatment.Methods High simulation inhomogeneous equivalent phantom of human body is used to simulate the typical patient after the operation of left breast cancer.The kay points and other points on behalf of depth to the region of interest with side of the chest wall are marked.Five radiotherapy plans (FIF-IMRT、IMRT、3DCRT、6 MeV-electron beam,9 MeV-electron beam) were designed separately on TPS based on phantom image series.After confirmed,the plans are delivered to the phantom and the dosimetrical quantities are measured.Using analysis of variance test the difference in the five methods.Results FIF-IMRT,IMRT,3DCRT,6 MeV-electron beam,9 MeV-electron beam,Five method actual measured doses respectively:Surface 74.32 cGy,69.21 cGy,73.97 cGy,75.86 cGy,81.4 1 cGy (F =3.36,P ＜ 0.05);0.5 cm Depth 95.59 cGy,93.37 cGy,96.78 cGy,99.63 cGy,94.97 cGy (F =2.40,P ＞ 0.05);1.0 cm Depth 103.42 cGy,102.53 cGy,103.48 cGy,88.89 cGy,101.36 cGy (F =7.19,P ＜ 0.05);Nearly chest wall of lung 82.74 cGy,68.24 c Gy,85.34 cGy,21.49 cGy,75.02 cGy (F =46.43,P ＜ 0.05).Compared to the dose value in TPS,dose delivered to Surface is lower at 8.04％ (-6.57％ to-11.93％),points at 0.5 cm is lower at 1.95％ (2.15％ to-5.90％),points at 1 cm is higher at0.65％ (-2.87％ to3.22％),lungislowerat3.53％ (3.90％ to-8.93％).Conclusions MOSFET detector with the corresponding simulation phantom can be used to measure the actual dose in a portion of body,and to evaluate the dosimetrical characteristics of different radiotherapy techniques.MOSFET detector is suitable for real-time,in vivo measurement of radiation dose during radiotherapy in breast cancer patients,so that the physicians are able to change treatment plan in time to ensure the accuracy of target dose.

Objective To study the sensitive function of CMNa on the pharyngeal cancer emission. Methods 120 pharyngeal cancer patients were randomly divided into the sensitive group and the control group with each group of 60. For sensitive group, CMNa 800 mg/m2 dosage in 30 min the vein drop note to the 60 min expert convention radiotherapy, 3 times each week, CMNa was used to the end of the radiotherapy. For control group, convention radiotherapy only. Results The CR rates of NP in sensitive group and control group were 88.3%and 73.3%(χ2=4.454, P=0.035). The CR rates of neck lymph node were 80.0%and 65.0%, respectively (χ2=3.419, P=0.064). There was no significant difference in toxicity between the two groups (P>0.05). Conclusion CMNa may increase the pharyngeal cancer therapy sensitivity, enhance the curative effects of pharyngeal cancer in the future, without serious response, but whether to reduce the whole mount of radiotherapy is still to be observed.

加速器产生的6-MV X-射线一次性照射体外培养的人肺腺癌细胞株A549和SPCA-1,细胞的吸收剂量分别设置为0、1、3、5和8 Gy,细胞照射后24h及单次接受5Gy照射后6、12和24h后取样;X-射线联合顺铂对BTG3表达的影响研究设立单纯照射组(5 Gy)、顺铂处理组(20 μmol·L-1顺铂)和联合处理组(5Gy+20 μmol.L-1顺铂),上述实验均以未给予任何处理的细胞为对照组;采用Western blot及RT-PCR法检测受到不同剂量电离辐射及照射后不同时间肿瘤细胞中BTG3表达水平.Western blot与PCR检测结果均显示,A549和SPCA-1细胞在受到不同剂量的X-射线照射后24h,其BTG3蛋白及mRNA表达量均明显增加,并随电离辐射剂量的增加而增加,与对照组比较有统计学差异(t=5.25-15.75,p＜0.05);照射后不同时间检测结果显示,细胞在X-射线照射4h后BTG3蛋白及mRNA的表达量即开始上升,并持续到照射后24 h,与照射前相比有统计学差异(t=7.52-11.18,p＜0.05);联合处理组BTG3的表达量均明显高于单纯照射组、顺铂处理组和对照组(t=7.02-15.86,p＜0.05).电离辐射联合顺铂可以上调肺癌细胞株中BTG3的表达,BTG3基因有可能作为肺癌放化疗的靶基因.

Objective To investigate the biological functions of IncRNA-BG on the radiosensitivity of normal human bronchial epithelial cell line Beas-2B.Methods Three IncRNA-BG siRNAs were designed,synthesized and traasfected into Beas-2B cells via lipofectamine.The RNA transcription level of BG was detected by quantitative real time-PCR to confirm the siRNA transfection efficiency.The experiment was divided into control group,control siRNA transfected group,and BG transfected group.Cell survival was detected by clonogenic assay,and the cell cycle distribution was determined by flow cytometry assay.The γ-H2AX foci formation after irradiation was visualized via immunofluorescence.Western blot assay was performed to detect the protein expressions of RAD50,p-P53,KU70,KU80,MDM2,CDK2 and RB.Results BG-siRNA transfection significantly reduced the BG transcription level (t =8.32-15.29,P ＜0.05) and increased cell survival after irradiation at 0.5,1,2,4 and 6 Gy.Analyzed with the multi-target model,the SERD0 of Beas-2B cells and control siRNA transfected cells were calculated to be 0.80 and 0.82,respectively.In addition,BG-siRNA transfection enhanced radiation-induced cell cycle arrest at G2 phase so that,after 4 Gy irradiation,the cells in G2 phase was increased from (37.37 ±0.63) ％ of control siRNA cells to (64.19 ± 1.01) ％ (t =30.65,P ＜ 0.05).Meanwhile,the γ-H2AX foci of BG-siRNA transfected cells was decreased from 76 ± 1.78 per 100 cells to 59-± 3.49 per 100 cells (t =13.72,P ＜0.05).The expressions of DNA damage related proteins including KU70,KUS0,CDK2 and RB were increased,but the expressions of p-P53 and RAD50 were decreased.Conclusions LncRNA-BG could regulate the radiosensitivity of the normal human bronchial epithelial cells,probably through inducing cell cycle G2 phase arrest and promoting DNA damage repair after irradiation.

苏州大学将人文素质教育融入到放射治疗技术学的教学中，全面挖掘肿瘤放疗技术学专业知识中的人文精神素材，积极探索如何在课堂和临床见习教学中适时融入人文素质教育，努力提高教学效果，培养高素质的医学人才。

Objective To analyze the effect of autophagy on celecoxib radiosensitizing human glioma SHG-44 cell line.Methods SHG-44 cells were divided into four groups:(1)control group(C);(2)celecoxib only(D);(3) radiation only(R);(4)celecoxib plus radiation(D+R).Celecoxib's radiosensitization was measured by clongenic assay.The cell cycle redistribution and apoptosis were analyzed by flow cytometric analysis.Meanwhile,acridine orange(AO),FITC-LC3-II antibody and TEM were used to detect autophagy.Results Celecoxib(30 μmol/L) radiosensitized SHG-44 cell by induced autophagy and cells G2/M arrest.Two days after 8 Gy irradiation or(and) celecoxib 30 μmol/L,SHG-44 cells showed by TEM prominent formation of autophagic vesicles with lamellar structures or residual digested material without nucleus condensation or segmentation.Next,cells were incubated by acridine orange or LC3-II antibody after irradiation or(and) celecoxib.The level of autophagy of(D+R) group was higher than that in the R alone(P0.05).The proportion of G2/M was(34.26±2.20)% of(D+R) treatment,compared with 8 Gy irradiation alone(29.15±1.99)%(P0.05).However,apoptotic cell death was(9.7±1.24)% and(8.2±0.93)% respectively without apparent statistic significance(P0.05).As the level of autophagy increased,the clongenic survival decreased exponentially(correlation index R=0.98,P0.05).Conclusion Celecoxib enhances SHG-44 cells G2/M phase arrest by radiation-induced and promoted cell autophagy.To induce autophagic cell death may be one of the mechanisms of celecoxib radiosensiting glioma cells SHG-44.

Objective To detect DNA damage of peripheral blood lymphocytes in patients and family members of autosomal dominant polycystic kidney disease (ADPKD),and to study the effect of irradiation on genomic stability of lymphocytes. Methods Before and after 0.5 Gy radiation dose,single-cell gel electrophoresis (SCGE) was employed to analyze DNA damage of lymphocytes in 10 ADPKD patients (group A),3 members without clinical symptoms of a ADPKD family (group B) and 20 healthy control people (group C).The damage was estimated based on the content of DNA in tail (TDNA％) with comet analysis software (CASP). Results Both before and after irradiation,the TDNA％ (8.85％±0.14％,14.84％±0.77％) and the value-added (6.00％±0.77％) of TDNA％ of group A were significantly higher than those of group C (7.50％±0.37％,12.46％±0.26％,4.96％±0.44％) respectively.There were no significant differences between group B and group C or group A and group B.While 1 person in group B had higher TDNA％ as compared to group C both before and after irradiation. Conclusions The lymphocytes of ADPKD patients are more sensitive to ionizing radiation as compared to healthy people.The genomic instability in ADPKD patients or member of ADPKD family may trigger cystic formation in multi-organs when exposing to environmental agents. SCGE may provide a new approach to elucidate the pathogenesis and prognosis of ADPKD.

目的 探讨血清癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)在肺癌诊断、临床分期以及病理分型中的作用.方法 采用化学发光法、放射免疫分析法和酶联免疫吸附法分别检测114 例肺癌患者和89例肺部良性疾病患者血清CEA、CYFRA21-1、NSE水平.结果 肺癌组血清CEA、CYFRA21-1、NSE含量明显高于肺良性疾病组(P＜0.05);CEA、CYFRA21-1及NSE诊断肺癌的ROC曲线下面积分别是0.60、0.85、0.82;联合检测对肺癌诊断的灵敏度明显高于任何单一检测(P＜0.05);Ⅰ+Ⅱ期肺癌组血清CEA含量明显高于肺良性疾病组(P＜0.05);CEA 在腺癌组含量明显高于鳞癌组(P＜0.05);NSE在小细胞肺癌组含量明显高于鳞癌组和腺癌组(P＜0.05);血清CEA在Ⅰ、Ⅱ、Ⅲ期非小细胞肺癌组含量明显低于Ⅳ期(P＜0.05);CYFRA21-1在Ⅰ、Ⅱ期非小细胞肺癌组含量明显低于Ⅲ、Ⅳ期(P＜0.05).结论 CEA对肺癌的早期诊断有一定意义;血清CEA对腺癌的灵敏度最佳,NSE对小细胞肺癌的灵敏度最佳;早期肺癌血清CEA、CYFRA21-1水平明显低于晚期肺癌;三种肿瘤标志物联合检测可明显提高肺癌诊断的敏感性,对肺癌的临床诊断、病理分型、临床分期具有重要的临床意义.

Objective To evaluate the radiosensitizing effect of Celecoxib,a selective cyclooxygenase-2 inhibitor,on colonic carcinoma cell line HT-29 in vivo and in vitro,and to probe the underlying mechanisms.Methods Colonic carcinoma cell line HT-29 was managed in vitro,and was treated by different concentration of Celecoxib,and the cell radiosensitivity was analyzed by colony formation unit assays;the change of tumor volume was observed by establishing the bear-tumor mice model of colonic carcinoma and drawing the tumor growth curve under different conditions;the expression of VEGF in colonic carcinoma tissues was detected by Immunohistochemistry assay.Results The colony formation unit assays showed SER was respectivly1.304 and 1.475 in different groups which were combined with Celecoxib(30μmol/ L and 50 μmol/ L).The tumor growth curve was used to do determination in these groups.When radiation was used combined with Celecoxib increase of tumor volume was the slowest.The expression level of VEGF in group Celecoxib was proved lower than that in the other groups(P0.05).Conclusion Celecoxib in vitro and in vivo could enhance the radiosensitivity in colon cancer carcinoma cell line HT-29 and inhibiting tumor angiogenesis maybe one of the underlying mechanisms.

Objective To investigate the effects and mechanisms of anti-proliferative protein family number-transducer of erbB2,1(TOB1) on breast cancer cell MDA-MB-231 metastasis.Methods The lipofectamine transfection and selective G418 cell culture were performed to obtain invasive breast cancer cell line MDA-MB-231,which over-expressed TOB1 protein.The effects on breast cancer cell invasion and migration in vitro were evaluated by basal membrane invasion assay and wound healing assay.Different expression of metastatic genes was evaluated via super array pathway-specific microarray.Results Compared with the control group,increased TOB1 expression significantly suppressed the invasion of MDA-MB-231(P0.05),and repressed MDA-MB-231 migration in vitro.Exogenous TOB1 up-regulated the expression of metastasis suppress gene BRMS1,while suppressed the mRNA expression of metastasis gene HSP90、FXYD5、RHOC、S100A4 in MDA-MB-231(all P0.05).Conclusion TOB1 over-expression will lead to the invasion and migration suppression of MDA-MB-231 in vitro,possibly by regulating the expression of metastasis related genes.

Objective To observe the effects of pycnogenol on contents of SOD and MDA,and the inhibition of superoxide anion and hydroxyl radical in 60Co γ-ray exposed mice organs.Methods The mice were randomly divided into 3 groups i.e.the normal control,irradiation control and pycnogenol experiment groups.The irradiation control and pycnogenol experiment groups were exposed to 60Co γ-ray,and the mice of pycnogenol experiment group were given pycnogenol for 7 days after radiation.The tissues were obtained after being sacrificed,in which the vitality of SOD,the content of MDA and the inhibition of superoxide anion and hydroxyl radical were detected.Results Results showed that after the mice were irradiated pycnogenol had no effect on SOD;however,the content of MDA could be reduced in organs.Pycnogenol could increase the inhibition of superoxide anion and hydroxyl radical in kidney,liver and testicular and could increase the inhibition of hydroxyl radical in spleen.Conclusions Pycnogenol shows certain anti-radiation effect through scavenging the superoxide anion and hydroxyl radical without increasing SOD content.