Characteristics of adult patients with high-grade gliomas.

Motility of Hsp70-positive cells emerging from a piece of tumor during cultivation for three days.

Motility of Hsp70-positive cells in a GBM tumor sample detected using laser scanning confocal microscopy during observation for 1 h.

Analysis of the mass-spectrometry data from isolated lipid rafts.

Grade content and co-localization of biomarkers (Hsp70, Nestin, SOX2) on histological preparations of human GBM.

Recently, the urgency of combating antibiotic-resistant bacteria, viruses, and other pathogens has dramatically increased. With the development of nanotechnology, significant hopes are placed on nanoparticles with antimicrobial properties. The efficiency of such materials can be significantly enhanced through light-activated processes. In this study, we prepared composite ZnO-Ag nanoparticles and tested their ability to inhibit Staphylococcus aureus bacteria. The composite ZnO-Ag nanoparticles were fabricated using pulsed laser ablation of Zn and Ag targets in water using a nanosecond pulsed laser. During antibacterial tests, light-enhanced activation of the nanoparticles was achieved using low-power near UV (375 nm) and blue visible (410 nm) LED irradiation. For comparison, similar laser-fabricated ZnO nanoparticles were also tested. The combined use of nanoparticles and LED irradiation significantly increased the generation of reactive oxygen species. As a result, low nanoparticle concentrations (0.05 g/L) and low-power LED irradiation (0.17-0.22 W) significantly reduced the concentration of Staphylococcus aureus bacteria, including experiments with visible light irradiation. Compared to their ZnO counterparts, the use of ZnO-Ag composite particles led to an additional increase in antimicrobial activity.

Motility of Hsp70-positive cells in a tumor sample.

Confocal microscopy images of primary glioblastoma cells from patients stained for mHsp70 and Nestin.

Characteristics of patients with brain metastases.

Gating of mHsp70High cells (field R2) and mHsp70Low cells (field R3) by FACS.

Effect of Hsp70 inhibitors PES and JG-98 on cell viability analyzed using the MTT assay.

Comparison of mean speed and track straightness on various matrices (PL, MG, FN) of patient-derived (non-sorted into high- and low-speed subpopulations) brain tumor cells treated with 1 µM PES or 50 nM JG-98.

The sequence of constructing a phenotyping map for histological sections of human GBM.

Confocal microscopy images of primary glioblastoma cells stained for mHsp70 and SOX2.

Motility of Hsp70-positive cells in a GBM tumor sample detected using laser scanning confocal microscopy during observation for 1 h, zoomed field of view from the center of the Video S1 frame.

Evaluation of biomarkers co-localization.

List of proteins detected by mass spectrometric analysis of lipid rafts isolated from normal brain tissue (obtained from a patient with epilepsy).

Purposeful synthesis of bismuth oxide nanoparticles (NPs) of various crystal modifications is important for biomedicine, photocatalysis and other applications. In this work, pulsed laser ablation of a metallic Bi target in atmospheric air is used to obtain (3- Bi 2 O 3 NPs. The effect of the Nd:YAG laser radiation power density (1064 nm, 7 ns) in the range of 0.1-1.2 GW/cm2 2 on the features of formation of NPs under nonequilibrium conditions is studied, for which the spectra of laser-induced plasma are recorded and analyzed, the plasma composition is determined, and the temperature of the plasma plume is estimated. The NPs are comprehensively characterized using TEM, XRD, FTIR, Raman, and UV-Vis spectroscopies, and electrophoretic light scattering, which make it possible to determine their morphology, crystal structure, chemical composition, and optical properties. The photocatalytic activity of the resulting nanopowders in the Rhodamine B dye decomposition reaction is assessed.

Cells of the TMS cell line in the well with the addition of a PES inhibitor on the matrigel matrix.

Automatic cell tracking before the AI model is applied. TMS cell line, a well without the addition of inhibitors on a Poly-L-lysine matrix.