BackgroundPrevious studies have reported abnormal expression of WNT1-inducible signaling pathway protein 1 (WISP1)/Cellular Communication Network Factor 4 (CCN4) in esophageal squamous cell carcinoma (ESCC). However, its specific significance remains unclear. To date, no in-depth research has been conducted to explore the role and importance of WISP1 in ESCC.MethodsIn this study, we downloaded the expression data of WISP1 (CCN4), Single-Cell RNA Sequencing (scRNA) data, and clinical information from public databases. A combination of bioinformatics analyses and experimental approaches was employed to comprehensively investigate the correlation between WISP1 expression and clinical prognosis, tumor microenvironment (TME), drug resistance, and response to immunotherapy. Additionally, the role of WISP1 in cancer-associated fibroblasts (CAFs) and its underlying mechanisms were explored.ResultsOur findings revealed that WISP1 exhibited differential expression in most analyzed cancers. In ESCC, WISP1 was upregulated and associated with TME characteristics, immune suppression, and drug resistance. Further analysis indicated that ESCC patients with higher WISP1 expression had relatively poorer prognoses. Moreover, it was confirmed that WISP1 is predominantly highly expressed in CAFs. Knockdown of WISP1 in CAFs significantly inhibited their proliferation, migration, and invasion capabilities, as well as markedly reduced the expression of extracellular matrix (ECM) proteins collagen type I alpha 1 chain (COL1A1) and matrix metallopeptidase 14 (MMP14). Notably, co-culture experiments of CAFs with knocked-down WISP1 and ESCC cancer cells demonstrated that the migration and invasion abilities of ESCC cancer cells were also significantly impaired.ConclusionIn summary, WISP1 is intricately involved in the pathogenesis of ESCC, exhibiting multifaceted roles. WISP1 can modulate the activities of CAFs and cancer cells in ESCC, as well as the process of ECM remodeling, thereby influencing the pathological progression of this malignancy. Based on the aforementioned research findings, WISP1 holds promise as a prognostic molecular marker and a potential therapeutic target for ESCC.

PH and Sect. 7 domain-containing protein 3 (PSD3) has been reported to be associated with some cancers, but its role in esophageal squamous cell carcinoma (ESCC) has not been thoroughly examined. The purpose of this study was to investigate the expression of PSD3 in ESCC and determine whether PSD3 is regulated by pyruvate kinase type M2 (PKM2) to affect the malignant phenotype of ESCC cells. First, we found that PSD3 was highly expressed in ESCC tissues and correlated with ESCC lymph node metastasis. In vitro, PSD3 promoted the proliferation, migration and invasion of ESCC cells. In vivo, PSD3 accelerated ESCC growth and metastasis. Next, the interaction between PSD3 and PKM2 was examined, and the results showed that PSD3 was directly regulated by PKM2. Functionally, PSD3 was regulated by PKM2 to promote the proliferation, migration and invasion of ESCC cells. Mechanistically, PSD3 was regulated by PKM2 to upregulate the expression of Vimentin and Snail and downregulate the expression of E-cadherin. Collectively, all the data we show here demonstrate that PSD3, regulated by PKM2, endows growth and metastasis advantages in ESCC by modulating epithelial-mesenchymal transition (EMT) progression.

Ulcerative colitis (UC) is a chronic and refractory inflammatory disease of the colon and rectum. This study utilized bioinformatics methods to explore the potential of Nicotinamide adenine dinucleotide (NAD+) metabolism-related genes (NMRGs) as key genes in UC. Using the GSE87466 dataset, differentially expressed NMRGs were identified through differential expression analysis, weighted gene co-expression network analysis (WGCNA), and NMRG scoring. These NMRGs were used as exposure factors, with UC as the outcome, to identify causal candidate genes through Mendelian randomization (MR) analysis. Key genes were further validated as biomarkers using machine learning and expression validation in external datasets (GSE75214, GSE224758). A nomogram based on the expression levels of these biomarkers was constructed to predict UC risk, and the biomarkers’ expression was validated through real-time quantitative polymerase chain reaction (RT-qPCR). Subsequently, signaling pathway analysis, enrichment analysis, immune infiltration analysis, and drug prediction were conducted to comprehensively understand the biological roles of the key genes in the human body. Single-cell (GSE116222) and spatial transcriptomic analyses (GSE189184) revealed the expression patterns of these key genes in specific cell types. NCF2, IL1B, S100A8, and SLC26A2 were identified as biomarkers, with NCF2 and IL1B serving as protective factors and S100A8 and SLC26A2 as risk factors for UC. The nomogram based on these biomarkers demonstrated strong predictive value. Functional analysis revealed significant IL1B, NCF2, and S100A8 enrichment in pathways such as IL-4 and IL-13 signaling, while SLC26A2 was strongly associated with respiratory electron transport. Significant differences in immune cells, such as macrophages and neutrophils, were also observed. Single-cell analysis showed high expression of NCF2, IL1B, and S100A8 in monocytes, while SLC26A2 was primarily expressed in epithelial cells, intestinal epithelial cells, and mast cells. Overall, these findings reveal the roles of NMRGs, providing valuable insights into the diagnosis and treatment of UC patients.

A 53-year-old man presented with progressive dysphagia and retrosternal pain. A gastroscope revealed a giant pronounced protrusion lesion with a thick pedicle in the lower esophagus measuring approximately 60 × 25 mm. Further refinement via computed tomography (CT) indicated an occupying lesion in the distal esophagus with no evidence of metastatic disease present; this led to the suggestion of performing an endoscopic resection

Understanding oncogenic processes and underlying mechanisms to advance research into human tumors is critical for effective treatment. Studies have shown that Metal regulatory transcription factor 2（MTF2) drives malignant progression in liver cancer and glioma. However, no systematic pan-cancer analysis of MTF2 has been performed. Here, we use University of California Santa Cruz, Cancer Genome Atlas , Genotype-Tissue Expression data, Tumor Immune Estimation Resource, and Clinical Proteomic Tumor Analysis Consortium bioinformatics tools to explore differential expression of MTF2 across different tumor types. MTF2 was found to be highly expressed in the cancer lines that were available through the respective databases included in the study, and overexpression of MTF2 may lead to a poor prognosis in tumor patients such as glioblastoma multiforme, brain lower grade glioma, KIPAN, LIHC, adrenocortical carcinoma, etc. We also validated MTF2 mutations in cancer, compared MTF2 methylation levels in normal and primary tumor tissues, analyzed the association of MTF2 with the immune microenvironment, and validated the functional role of MTF2 in glioma U87 and U251 and breast cancer MDA-MB-231 cell lines by cytometry. This also indicates that MTF2 has a promising application prospect in cancer treatment.

目的 分析食管鳞状细胞癌中基因组的遗传学变异特征,探讨异常扩增基因PPFIA1与临床病理参数及预后的相关性.方法 选取2014年3月—2014年7月新疆医科大学第一附属医院食管鳞状细胞癌患者手术切除的6对肿瘤组织及癌旁正常组织.采用比较基因组杂交技术(CGH)检测食管鳞状细胞癌患者基因组遗传学变化,免疫组织化学法检测异常扩增基因PPFIA1在食管鳞状细胞癌中的表达水平,分析PPFIA1基因与食管鳞状细胞癌病理参数及预后的相关性.结果 CGH分析结果发现食管鳞状细胞癌患者中5号染色体p15.33和11号染色体的q13.3和q22.1变异较大.食管癌组织PPFIA1阳性表达率高于癌旁组织(P<0.05).不同临床分期、淋巴结转移食管癌患者的PPFIA1高表达率比较,差异有统计学意义(P<0.05),临床分期越高PPFIA1的阳性表达率也越高,淋巴结有转移的患者PPFIA1的阳性表达率高于无淋巴结转移的患者.而不同性别、年龄、病理分级、分化类型、肿瘤大小、浸润深度食管癌患者的PPFIA1高表达率比较,差异无统计学意义(P>0.05).PPFIA1高表达与低表达患者生存曲线比较,差异有统计学意义(P<0.05),PPFIA1高表达患者生存期较短,预后较差.结论 食管鳞状细胞癌中11q13.3变异较大,PPFIA1基因可能在食管鳞状细胞癌的转移和预后中起一定作用.

目的 探究ω-3多不饱和脂肪酸(ω-3PUFAs)对炎症性肠病(IBD)患者免疫细胞功能及炎性指标的影响.方法 选取2019年10月2020年12月新疆医科大学第五附属医院消化科120例IBD患者,随机分为观察组和对照组,各60例.对照组患者实施美沙拉嗪治疗方案,观察组患者在对照组基础上予以ω-3PUFAs制剂治疗.比较两组治疗10周后疗效表现,比较两组治疗前及治疗10周后免疫细胞功能(CD3+、CD4+、CD8+、NK细胞)、炎性指标[肿瘤坏死因子(TNF-α)、C反应蛋白(CRP)、白介素-6(IL-6)]、肠屏障功能[二胺氧化酶(DAO)、D-乳酸(D-LA)、内毒素(ET)]、肠道菌群(双歧杆菌、大肠埃希菌、乳酸杆菌、肠球菌)水平.结果 治疗10周后,观察组总有效率显著高于对照组(P均<0.05);两组CD3+、CD4+、NK细胞、双歧杆菌、乳酸杆菌水平均较治疗前显著升高,且观察组明显高于对照组(P均<0.05);两组CD8+、TNF-α、CRP、IL-6、DAO、D-LA、ET、大肠埃希菌、肠球菌水平均较治疗前显著下降,且观察组显著低于对照组(P均<0.05).结论 ω-3PUFAs可有效调节IBD患者免疫细胞功能,并抑制其炎症状态,同时可显著改善患者肠屏障功能,调节其肠道菌群环境,具有较高的临床应用价值.

Tumor-infiltrating lymphocytes (TILs), including but not limited to neutrophils, M2 macrophages, cytotoxic CD8 T cells and dendritic cells, will play a role in the acidic tumor microenvironment mediated by monocarboxylate transporter 4 (MCT4) in esophageal squamous cell carcinoma (ESCC). However, the roles they play and their significance in ESCC remain less clear. To understand the clinicopathological and prognostic significance of neutrophils, M2 macrophages, CD8 T cells and dendritic cells in the tumor acidic microenvironment mediated by MCT4, we investigated the distribution of these TILs in the epithelial and stromal compartments of ESCC by means of multiplexed immunohistochemistry on a tissue microarray containing 87 paired dots of ESCC and its adjacent normal tissue (ANT) and an additional 6 cases of unpaired ESCC dots. The density of cells stained with MCT4 in the epithelium was significantly associated with overall survival. Dendritic cells stained with S100 in epithelial compartmentalization were found to markedly correlate with clinical stage and tumor invasion depth. No other significant association could be identified in terms of prognostic and clinicopathological significance. The potential correlation between the number of cells stained with MCT4 versus the number of TILs was also explored, showing that only in epithelial cells were there significant and positive correlations identified between the number of cells stained with MCT4 versus the number of neutrophils stained with CD15, M2 macrophages stained with CD163 and CD8 T cells stained by CD8a. However, no significant correlation was found along the stromal line. Together, the data we described here, although somewhat discouraging, showed that in epithelial cells from which ESCC originated, acidicity mediated by MCT4 may be responsible for lactate release and may have an effect on the infiltration of TILs we assessed.

Subsequently to the publication of the above article, the authors have realized that Fig. 5B on p. 8 was compiled erroneously, in the sense that the two immunohistochemical images selected for Fig 5B did not correspond to each other, meaning they were not derived from the same field under the microscope. This error was inadvertently made during the preparation of the manuscript. A corrected version of Fig. 5, showing the correct data for the expression of PKM2 in NAT in Fig. 5B, is shown on the next page. This inadvertent error did not affect the conclusions reported in this paper, and all the authors agree with this Corrigendum. The authors sincerely thank the Editor of Oncology Reports for presenting them with the opportunity to publish this Corrigendum, and apologize to the readership of the journal for any inconvenience caused. [the original article was published in Oncology Reports 46: Article no. 216, 2021; DOI: 10.3892/or.2021.8167].

目的 探讨食管鳞癌细胞KYSE150外泌体对细胞增殖、侵袭迁移的影响.方法 培养KYSE150并用试剂盒法提取外泌体,采用透射电镜、纳米粒子示踪分析、蛋白免疫印迹法、吞噬实验等对外泌体进行鉴定.将外泌体与KYSE150共培养,采用细胞划痕实验比较外泌体对细胞增殖能力的影响;采用Transwell实验比较外泌体对细胞迁移能力的影响.结果 提取后的外泌体经过纳米粒子示踪分析提示颗粒大小30～200 nm,电镜形态正常,免疫印迹实验显示CD9和CD81均有条带出现,且外泌体可被自身吞噬.与PBS对照组的细胞迁移率(47.67±2.52)％和迁移细胞数(147.33±5.89)个比较,外泌体实验组的细胞迁移率(64.67±3.05)％和迁移细胞数(233.22±4.58)个明显增高,差异有统计学意义(P<0.05),提示外泌体实验组的细胞增殖与侵袭迁移能力增强.结论 外泌体能显著促进食管鳞癌细胞运动能力.

Background: Shikonin, a small molecule inhibitor of pyruvate kinase 2 (PKM2), has been demonstrated to play the antitumor effect in various cancers.However, the specific effects and related regulatory mechanism of Shikonin in esophageal squamous cell carcinoma (ESCC) have not been clearly declared.Materials and methods: Cell viability was valued through 3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay.Glucose consumption, lactate production, glycolytic intermediates and pyruvate kinase enzymatic activity were measured using corresponding assay kits.Patient-derived xenograft (PDX) models were constructed to observe the anti-ESCC effect of Shikonin in vivo.PKM2, p-PKM2, signal transducer and activator of transcription 3 (STAT3), p-STAT3, glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) in ESCC tissues were assessed by western blot.The expression of PKM2, p-PKM2, p-STAT3, GLUT1 and HK2 was assessed by immunohistochemistry (IHC) in ESCC tissue based on PDXs.Results: Shikonin effectively inhibited cell proliferation in dose-dependent and time-dependent manner compared with the control group.The detection of glycolysis showed that Shikonin suppressed the glucose consumption, lactate production, glycolytic intermediates and pyruvate kinase enzymatic activity.Furthermore, Shikonin not only inhibited the growth of ESCC, but also decreased the expression of p-PKM2 and p-STAT3 in vivo.Finally, Shikonin suppressed the expression of GLUT1 and HK2 proteins which are related to glycolysis.Conclusion: Shikonin has a significant antitumor effect in the ESCC by suppressing PKM2 mediated aerobic glycolysis and regulating PKM2/STAT3 signal pathway.

目的 采用数学模型对某地社区卫生服务中心卫生人员及床位的配置和使用的发展趋势进行分析与定量预测,了解2018-2025年社区卫生服务中心卫生服务能力发展情况.方法 依据2010-2017年当地卫生统计年鉴相关数据,应用Verhulst数学模型预测2018-2025年社区卫生服务中心卫生人员和床位配置及使用情况.结果 2010-2017年,社区卫生服务中心卫生人员总数除2016年外其余呈增长趋势,2013年增长幅度最大为11.6％,卫技人员与非卫技人员比例呈S型变化,医护比、卫技人员与床位数比例均上升;平均开放病床数、病床周转次数、病床使用率及每千人床位数均呈下降趋势;通过灰色Verhulst预测模型预测2018-2025年,中心卫生人员数、卫技人员比例、医护比及卫技人员与床位数比例均上升,非卫技人员、平均开放病床数、病床周转次数及病床使用率、每千人床位数均呈逐年下降趋势.结论 2010-2017年卫技人员的比例均达到国家大于80％的标准;非卫技人员比例除2014与2015年,其余年份略高于国家15％的标准;医护比达到国家1:1的标准;卫技人员与床位数比例均已超过国家标准1.2:1;到2017年每千人床位数有0.22张,根据预测推算出2025年每千人床位数有0.191张,但远达不到国家0.3-0.6张的标准.

目的 探讨长非编码RNA(Long noncoding RNA,LncRNA)H19介导食管癌细胞上皮间质转化(Epithelial-Mesenchymal Transition,EMT)的作用及机制.方法 收集食管癌临床组织样本48例,通过qRT-PCR检测癌组织及癌旁组织中H19和let-7的表达,同时统计分析H19与食管癌患者临床病理参数及术后生存时间的相关性.通过慢病毒转染敲低H19在食管癌细胞中的表达水平,分别运用Western blot和qRT-PCR,检测EMT相关蛋白及微小RNA(microRNA,miRNA)let-7的表达.结果 qRT-PCR检测发现食管癌组织中H19表达水平显著上调,且与肿瘤浸润深度及预后不良相关(P<0.05).Western blot检测发现敲低H19表达后,食管癌细胞中E-cadherin表达上调,β-catenin、Vimentin和Slug表达均下调,与空白对照组和阴性对照组比较差异均有统计学意义(P<0.05).H19在食管癌组织中高表达的同时,let-7表达下调(P<0.05),在细胞水平敲低H19的表达后,let-7表达增多,与空白对照组和阴性对照组比较差异均有统计学意义(P<0.05).结论 H19可能通过负调控let-7的表达,从而介导食管癌EMT过程.

目的 探讨促炎细胞因子白介素1β(IL-1β)对食管鳞癌细胞程序性细胞死亡1配体1(PD-L1)的表达调控作用及调控机制,阐明IL-1β在食管鳞癌肿瘤微环境中调控PD-L1表达的临床意义.方法 分析TCGA数据中收录的有关食管癌中IL-1β和PD-L1表达的数据,明确IL-1β和PD-L1在食管癌中的表达及预后意义;在体外细胞系水平,运用重组的人源化IL-β蛋白刺激食管鳞癌细胞系KYSE30后,运用qRT-PCR检测PD-L1的mRNA表达,运用免疫印迹和免疫荧光实验技术检测PD-L1蛋白表达;为了明确IL-β的作用信号通路,运用抗体芯片筛选重组的人源化IL-β蛋白处理和未处理食管癌细胞内的信号通路差异.结果 生物信息学分析显示,相比癌旁正常组织IL-1β和PD-L1在食管癌组织中显著性上调表达;但上调的IL-1β和PD-L1表达与食管癌患者的总生存差具有统计学趋势但不具有统计学相关性.重组的人源化IL-β蛋白刺激食管鳞癌细胞系后,能显著性上调PD-L1的mRNA和蛋白表达.抗体芯片筛选结果显示,重组的人源化IL-β蛋白处理食管癌细胞后,能显著性激活STAT3信号通路.结论 IL-1β通过STAT3信号通路上调食管鳞癌细胞PD-L1表达.

背景 随着大众健康观念的转变,人群健康管理需求和消费能力有所提升,在当前供给侧结构性改革的大背景下,如何从供需两端发力,对居民健康管理消费需求和行为加以合理的引导,成为亟待研究的问题.目的 通过了解居民选择健康管理服务时的权衡过程及其偏好,以居民真实的健康管理服务需求为依据,为政策制定者和健康管理服务供给方提供决策依据.方法 采用简单随机抽样和方便抽样相结合的方法于2019年7—10月在乌鲁木齐市7个区展开离散选择实验问卷调查.问卷包括调查对象基本信息、离散选择实验选择集(共包括服务主体、服务内容、线上服务提供情况、中医药服务提供情况、家庭医生签约服务提供情况、自付费用6个属性,每个属性又包括若干水平).运用Stata 15.0软件构建离散选择模型,采用混合Logit回归模型分析居民健康管理服务选择偏好.结果 共发放问卷945份,回收有效问卷802份,有效回收率为84.9％.离散选择模型结果显示,居民效用最高的健康管理服务组合方案为公立医院健康管理部门、健康体检+健康教育与咨询或健康体检+健康干预、丰富的线上服务、有中医药服务、有家庭医生签约服务(P<0.05).结论 乌鲁木齐市居民在选择健康管理服务时最关心服务主体,对服务内容的潜在需求仍需引导,居民对线上服务、中医药服务和家庭医生签约服务存在偏好.目前应该加大宣传力度和政策指引,充分发挥各类健康管理机构在健康管理中的作用,完善线上线下的服务闭环,积极引进中医慢性病防控的服务方式,持续推进家庭医生签约服务的有序进行.

目的 分析与预测新疆社区卫生服务中心医疗服务效率的发展趋势,找出与西部地区之间存在的差距,为提升新疆社区卫生服务中心医疗服务效率提供依据.方法 基于2011-2019年新疆统计年鉴及国家卫生统计年鉴相关数据,借助灰色预测GM(1,1)模型分析预测2019-2025年新疆社区卫生服务中心医疗服务效率.结果 2010-2018年,新疆社区卫生服务中心诊疗人次数及医师日均担负诊疗人次数均呈逐年增长趋势,截止至2018年,分别为603.0万人次及11.9人次;床位使用率、出院人数、出院患者平均住院日及医师日均担负住院床日数均呈S型变化,截止至2018年,分别为42.9％、4.7万人、8.0日及0.8床日.通过灰色模型预测得,2019-2025年诊疗人次数、医师日均担负诊疗人次数、出院人数均呈上升趋势,预测至2025年,分别增加到1106.04万人次、15.73人次及4.81万人次;病床使用率、平均住院日、医师日均担负住院床日均呈下降趋势,预测至2025年分别下降到39.52％、7.07日及0.77床日.结论 未来几年,新疆社区卫生服务中心的医疗服务效率仍处于西部地区中等偏下水平,政府应根据当地实际情况在提升诊疗服务效率的基础上,加大对住院服务效率的提升.

Exosomal pyruvate kinase isoenzyme type M2 (PKM2) has been found to play a key role in the progression of human hepatocarcinoma. However, exosomal PKM2 (especially plasma-derived exosomal PKM2), in patients with oesophageal squamous cell carcinoma (ESCC) has not been well defined. In the present study, plasma-derived exosomes were isolated from healthy controls and patients with ESCC, and identified by transmission electronic microscopy, western blotting, nano-flow cytometry, nanoparticle tracking and phagocytosis analysis; exosomal PKM2 was detected by western blotting and ELISA. In addition, changes in cellular proliferation and motility in recipient cells (Eca109) were assessed using Cell Counting Kit-8, colony formation, wound-healing and Transwell assays. The PKM2 content was higher in exosomes from patients with ESCC than in those from healthy donors. Furthermore, exosomes from patients with ESCC enhanced the proliferation and motility of ESCC cells in vitro. Notably, PKM2 was found to be transferred by exosomes, and was able to act by activating STAT3. To verify the association between PKM2 and STAT3, immunohistochemistry was employed to analyse the protein levels of PKM2 and pSTAT3(Tyr705). These data revealed that PKM2 and pSTAT3(Tyr705) were upregulated and associated with overall survival in patients with ESCC. Therefore, the present study highlights that exosomes from patients with ESCC enhance the migration and invasiveness of ESCC cells by transferring PKM2.

Background Pyruvate kinase 2 (PKM2) is a key enzyme in the glycolysis pathway and has been reported to be associated with the development of esophageal squamous cell carcinoma (ESCC). However, the prognostic value of PKM2 in ESCC remains undetermined. Methods This study aimed to investigate the clinicopathological significance of PKM2 expression in ESCC. A comprehensive and systematic literature search was conducted using the PubMed, Embase, Medline, and Cochrane library databases. The quality of studies and potential for bias were appraised, and meta-analysis was performed to assess the prognostic impact of PKM2 on overall survival (OS). Results A total of 5 studies with 781 participants were eligible and enrolled. Patients with high PKM2 expression were associated with poor prognosis in ESCC [hazard ratio (HR) =1.72, 95% confidence interval (CI): 1.41–2.09; P<0.01]. Furthermore, upregulated PKM2 was significantly associated with lymph node metastasis [odds ratio (OR) =2.38, 95% CI: 1.68–3.35; P<0.01], clinical stage (OR =3.29, 95% CI: 2.27–4.77; P<0.01), and tumor (T) classification (OR =2.92, 95% CI: 2.05–4.16, P<0.01). Discussion High PKM2 expression denotes worse OS in ESCC patients, and correlates with the lymph node metastasis, clinical stage, and T classification. However, further studies are warranted to assess how PKM2 can be implemented as a reliable staging element in clinical practice and whether it could provide a new target for therapeutic intervention.

Objective To investigate the awareness and vaccination willingness of human papillomavirus(HPV) vaccine among college students in Urumqi,analyze the factors affecting HPV vaccination,and provide a reference for further promoting the HPV vaccination among college students.Methods From April to May 2020,after a stratified cluster sampling of freshman to junior college students aged ≥18 years old in a university in Urumqi,an electronic questionnaire survey was conducted on HPV vaccine awareness and vaccination willingness.Results A total of 590 valid questionnaires were collected,including 211(35.76%) in the first year,207(35.08%) in the second year,and 172(29.15%) in the third year. There were 203 people(34.41%) who had heard of HPV and 216 people(36.61%) who had heard of HPV vaccine. There were statistically significant differences in HPV and HPV vaccine awareness among college students of different ages,grades,household registration,and premarital sexual behaviors(all P<0.05).There were 461 people(78.14%) who were willing to receive HPV vaccine. The main reason for the vaccination was that they thought they could benefit from it and accounted for 76.79%(354 people). 129 people(21.86%) were unwilling to receive HPV vaccine. The main question was in terms of vaccine safety,which accounted for 53.49%(69 people). Logistic regression analysis showed that the factors affecting the willingness of HPV vaccination were grade,gender and attitudes towards premarital sex(all P<0.05). Conclusion College students have a low awareness of the HPV-related,but the willingness to vaccinate is considerable.Therefore,in order to improve the awareness of the HPV vaccine among college students and further increase the willingness to vaccinate,it is necessary to use the university platform to strengthen the promotion of HPV vaccine health-related knowledge.