OBJECTIVE:The study was designed to investigate how varying ratios of a functional monomer, 4-formylphenyl acrylate (FA) primer, formulated with a blend of ethyl-acetate (EAC) and ethanol (ET), impact the adhesive durability of All Bond Universal (ABU) and Single Bond Universal (SBU) under both etching and rinsing modes. METHODS:FA was synthesized via the acyl chloride reaction. FA primer was prepared by mixing ethyl acetate and ethanol as a binary solvent system at solvent ratios of 7:3, 6:4, 5:5, and 4:6. The optimal ratio of ethyl acetate to ethanol was determined by measuring the degree of conversion (DC) and contact angle, combining the microtensile bond strength (µTBS) and fracture pattern immediately and 10,000 thermocycles. Matrix metalloproteinases (MMPs) activities and adhesive infiltration at the interface were observed, whereas scanning electron microscopy was used to further evaluate nanoleakage and resin rags. RESULTS:Application of the primer containing a mixed EAC:ET-4:6 solvent resulted in a significant enhancement in the µTBS of both immediate and aging groups, and the contact angle of the demineralized dentin surface. Confocal Laser Scanning Microscope (CLSM) and Scanning Electron Microscope (SEM) were further observed the infiltration properties of resin rags in the bonding interface were strengthened. The MMPs activities in the hybrid layer was clearly inhibited, and the degree of nanoleakage at the bonding interface reduced remarkably, thus, it improved the stability and durability of the bond. CONCLUSION:FA primers containing appropriate ratios of ethanol and ethyl acetate solvents can simplify operation procedures, enhances collagen fiber resistance to enzymatic degradation, and improves hybrid layer sealing, thereby increasing bonding strength. CLINICAL SIGNIFICANCE:The FA primer formulated with an ethyl acetate-ethanol mixed solvent aim to achieve high-strength, low leakage, and long-term durable adhesive effects, demonstrating dual functional advantages: Effective collagen crosslinking capacity leading to enhanced bond durability and reduced secondary caries incidence, alongside decreased technique sensitivity. These findings provide novel perspectives for FA primer applications in clinical practice.

Objective: To prepare antibacterial resin cement containing photocurable triclosan derivative, and to evaluate its characterization performance, mechanical properties, adhesive strength, antibacterial properties, and cytotoxicity in vitro. Methods: The photocurable triclosan derivative（TCS-IH） was dissolved in acetone at 25 wt% and then added to Multilink Speed at a mass ratio of 0 wt%, 2 wt%, 4 wt%, 6 wt%, and 8 wt%, to obtain the resin cement with antibacterial properties for different components. FTIR was used to examine the conversion of double bonds before and after curing of resin cement in each group. The surface wettability of resin cement in each group was examined by static water contact angle. Its flexure strength was tested with a three-point bending test. Micro-tensile test was used to examine its instant bonding properties. S.mutans was selected for antibacterial experiments, and the antibacterial properties of resin cements were evaluated by colony counting, MTT, and live/dead bacterial fluorescence staining. The cytotoxicity grade in vitro was evaluated by CCK-8 cytotoxicity assay. Results: The addition of TCS-IH acetone solution at 2 wt% and 4 wt% had no significant effect on characterization performance, mechanical properties, and bonding strength of resin cement（P＞0.05）. When the mass percentage of TCS-IH reached 4 wt%, 6 wt%, and 8 wt%, resin cement all showed significant antimicrobial activity（P＜0.05）. Both 2 wt% and 4 wt% resin cements had good biocompatibility. Conclusion: When the mass percentage of TCS-IH was 4 wt%, the antibacterial properties of resin cement were effectively improved while its characterization properties, mechanical properties, adhesive properties, and biosafety were not affected.

牙本质粘接依靠粘接剂渗透到脱矿的胶原纤维网状结构和牙本质小管内并原位聚合,形成混合层和树脂突获得主要粘接固位力[11.牙本质因有机物含量高和粘接剂中聚合物降解等因素难以形成高质量的混合层[1～4].粘接界面是牙本质粘接的薄弱环节,其混合层质量及抗老化性能是影响粘接耐久性的关键.

有限元分析是利用数学计算分析真实的物理系统,计算模型赋予材料的参数,将任意几何结构分成有限个小的单元体,之间通过节点连接,计算每个结构中的形变、应力和所产生的变量,从而模拟分析真实条件情况[1,2].并可对具有复杂性质材料组成的不规则几何结构进行建模,模拟出复杂载荷与边界条件的情况[3,4].使用这种方法,可分析获得口腔修复材料的理想特性[5].

This review aims to evaluate whether the etch-and-rinse or self-etch mode is the better protocol for dentin adhesion by universal adhesives. A total of 15 articles were included in the meta-analysis. Two reviewers performed a literature search up to October 2020in four databases: PubMed, Web of Science, Embase, and the Cochrane Library. Without considering the difference in aging mode, the analysis of the immediate and long-term bond strength of dentin showed that there was no statistical significance between the etch-and-rinse and self-etch mode of universal adhesive, and the long-term bond strength decreased relative to the immediate. In vitro studies suggest that prior acid etching did not improve bond performance. Whether from the perspective of long-term bonding performance or simplifying operating procedures, the self-etch mode is preferred.

近几十年,牙本质粘接剂历经多代改良趋向多功能化和操作简便化,并在口腔修复治疗中广泛应用.为获得理想的远期修复效果,牙本质粘接剂在具备良好的物理化学和机械性能基础上,还需具备有效的抗龋性能.该文将重点从抗菌肽的抗菌作用机制、抗菌肽的种类、在牙本质粘接剂中的应用及防龋效果等方面展开综述,并对抗菌肽在牙本质粘接剂应用中面临的挑战和发展前景作出展望.

Objectives For a conventional indirect restoration, temporary cementation inevitably contaminated collapsed dentin collagen. The purpose of this review was to evaluate the optimal strategy for minimizing its negative effects. Material and methods Databases such as PubMed, Web of Science, EMBASE, and the Cochrane Library were searched for in vitro studies, involving the influence of immediate dentin sealing (IDS), different temporary cements, and their removal strategies on dentin bond strength. The meta-analysis used the inverse variance method with effect method of the standardized mean difference and statistical significance at p ≤ 0.05. The I 2 value and the Q -test were used to assess the heterogeneity. Results A total of 14 in vitro trials were subjected to the meta-analysis. Within the study’s limitations, we assumed that IDS eliminated the negative effects of temporary bonding, achieving the comparable immediate bond strength with the control ( p = 0.46). In contrast, under delayed dentin sealing (DDS), temporary cementation statistically decreased bond strength ( p = 0.002). Compared with resin-based and non-eugenol zinc oxide cements, polycarboxylate and calcium hydroxide cements performed better on bond strength with no statistical difference from the control group ( p > 0.05). Among the removal methods of temporary cements, the Al 2 O 3 abrasion restored the decreased bond strength ( p = 0.07) and performed better than hand instruments alone ( p = 0.04), while pumice removal slightly reduced the bond strength in contrast with the control group ( p = 0.05, 95% CI = − 1.62 to 0). Conclusions The choices of IDS, polycarboxylate and calcium hydroxide temporary cements, Al 2 O 3 abrasion removal method were feasible and efficient to enhance the bond strength. Clinical relevance It is worthwhile applying IDS technique, polycarboxylate and calcium hydroxide temporary cements during indirect restoration. The Al 2 O 3 abrasion of cleaning dentin can minimize the negative effects of temporary cement.

光照射后固化因为有利于大块充填树脂各种性能的提高,日益引起关注.其中光照射后固化对于提高复合树脂双键转化率、增加硬度、减小聚合收缩应力均有较好的效果.温度、光固化反应的双键转化率、树脂组成成分和浸泡溶剂等因素都可以影响大块充填树脂的光照射后固化.本文将对以上内容及其对临床意义进行综述.

成功的修复体必须具备功能性和耐久性,提高树脂-牙本质粘接界面耐水解和酶促降解对于提高粘接修复的耐久性至关重要.粘接界面再矿化有助于解决该问题.该文主要对仿生类似物诱导树脂-牙本质粘接界面再矿化的机制、常用产物及其表征做一综述.

Pickles are popular in China and exhibits health-promoting effects. However, nitrite produced during fermentation adversely affects health due to formation of methemoglobin and conversion to carcinogenic nitrosamine. Fruiting bodies of the mushroom Boletus edulis were capable of inhibiting nitrite production during pickle fermentation. A 90-kDa nitrite reductase (NiR), demonstrating peptide sequence homology to fungal nitrite reductase, was isolated from B. edulis fruiting bodies. The optimum temperature and pH of the enzyme was 45 °C and 6.8, respectively. B. edulis NiR was capable of prolonging the lifespan of nitrite-intoxicated mice, indicating that it had the action of an antidote. The enzyme could also eliminate nitrite from blood after intragastric administration of sodium nitrite and after packaging into capsule, this nitrite-eliminating activity could persist for at least 120 minutes thus avoiding immediate gastric degradation. B. edulis NiR represents the first nitrite reductase purified from mushrooms and may facilitate subsequent applications.

An RNase with a molecular mass of 28 kDa and with high ribonucleolytic activity toward poly(A) was purified from the ascocarps of Tuber indicum. The purification procedure involved ion exchange chromatography on diethylaminoethyl cellulose, Q‐Sepharose and Mono Q, and gel filtration by fast protein liquid chromatography on Superdex 75. The pH and temperature optima of the RNase were 7.2 and 50 °C, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly(A)>poly(C)>poly(G) ≈ poly(U). All of the metal ions used in this study, except for the K+ ions, curtailed the activity of the RNase. The RNase activity was reduced by ethylene diamine tetraacetic acid (EDTA), dithiothreitol (DTT), and sodium dodecyl sulfate (SDS) by 42.2%, 75.5%, and 96.6%, respectively. The RNase inhibited the proliferation of hepatoma (HepG2) and human breast cancer cell lines (MCF7), with half‐maximal inhibitory concentrations (IC50) of 12.6 and 16.6 μM, respectively.

A bacterial isolate,WM1002,obtained from packaged Pleurotus geesterani fruit bodies that had been processed using super high pressure technology,was identified as a strain of Bacillus based on 16S rRNA sequencing.The 16S rRNA sequence has been deposited in GenBank under the accession number,HQ730134.A phylogenetic tree consisting of 16S rRNA sequences from other Bacillus species and constructed using the neighbor-joining method revealed that isolate WM1002 exhibited a high genetic similarity(99.8%) with B.velezensis CR-502,B.vallismortis DSM11031,B.methylotrophicus CBMB205 and B.atrophaeus JCM9070.

[Objective] To isolate and identify a spoilage bacteria in the ultra-high pressure preserved Lentinula edodes foods.[Method] Spoilage microorganisms in ultra-high pressure treated Lentinula edodes food were isolated by diluted plate and streak cultivation methods.And the morphological,physiological,biochemical properties,16S rDNA sequence and phylogenetic tree of the isolated strains were analyzed.[Result] A strain of Bacillus sp.(No.MW1001) was isolated from the treated shiitake mushroom samples.Based on the results of 16S rDNA sequencing,and physiological and biochemical properties,the strain showed high genetic relation to Bacillus methylotrophicus and Bacillus subtilis with the homology of 99.55% and 99.24% respectively.[Condusion]The research provided theoretical basis for further improving the ultra-high pressure preservation of Lentinula edodes.

The aim of this study is to identify the bacteria strain numbered as WM1003 isolated from the package of the Agrocybe cylindracea processed by super high pressure technology. The genomic DNA of the strain WM1003 was isolated and purified. 16S rRNA gene sequence was amplified using genomic DNA of the strain WM1003 as template and was sequenced. Phylogenetic position of the WM1003 was analyzed by using MEGA4.0 based on the 16S rRNA sequences. PCR product of the strain WM1003 was about 1600 bp. The 16S rRNA gene sequence of the strain WM1003 was accepted by GenBank as HM748831. Phylogenetic analysis based on the 16S rRNA sequences showed that the strain WM1003 held highest similarity with Paenibacillus jamilae CECT 5266 (99.5%). In the present study, the strain WM1003 was identified through 16S rRNA gene sequence and phylogenetic analysis. This laid the foundation for the application of super high pressurs technology in Agrocybe cylindracea.