目的 观察银黄消痤胶囊对痤疮家兔模型皮脂腺及P物质(Substance P,SP)的影响.方法 70只12～13 w龄雄性家兔,随机平均分为空白组、模型组、银黄消痤胶囊高、中、低剂量组、清热暗疮丸组、盐酸米诺环素组7组.除空白组外,其余60只以2％煤焦油溶液涂抹家兔左耳2 w建立痤疮模型.造模成功后,在给药前及给药3 w后,分别在兔耳动脉抽血用酶联免疫吸附法(ELISA)测血清SP含量及取左耳组织行病理检查.结果 各组实验性粉刺组织学分级比较,治疗后各组与模型组比较,均具有统计学意义(P<0.01).血清SP检测:治疗后银黄消痤胶囊高、中、低剂量组、清热暗疮丸组、米诺环素组与模型组比较有极显著降低(P<0.01);消痤胶囊高剂量组比较中剂量、米诺环素组有显著降低(P<0.05),高剂量组与低剂量组、清热暗疮丸组比较有极显著降低(P<0.01);中剂量组与清热暗疮丸组比较有显著降低(P<0.05).结论 银黄消痤胶囊可降低家兔痤疮模型血清SP含量,银黄消痤胶囊可明显抑制毛囊皮脂腺导管角化异常,表明银黄消痤胶囊可通过降低SP含量及抑制毛囊皮脂腺导管角化异常来治疗痤疮.

Based on computer network platform to explore the impact of the heuristic teaching of medical records and effective classroom questioning on the teaching of Surgery of Traditional Chinese medicine. For this study, 195 undergraduates of traditional Chinese medicine specialty, from the classes of 2014, 2015 and 2016, from Shaanxi University of Traditional Chinese Medicine, were selected as research objects. The plan was to teach three heuristic medical records in the 2014class; seven were planned for the 2015 class. The effectiveness of the construction of questions related to heuristic medical records for the 2016 class grades were based on the grades from 2015.The changes of test scores, grade points and examination grades were analyzed before and after the increase of heuristic medical records and effective classroom questioning. The test scores and grade points gradually but significantly increased from the classes of 2014, 2015 and 2016 (P < 0.05).The test scores and grade points of the 2015 and 2016classes were significantly higher than the 2014 class (P < 0.05), while those of the 2016class were obviously higher than the 2014 class (P < 0.05). The proportions of students with good grades and above in 2014, 2015 and 2016 were 12.00%, 44.68% and 87.176% respectively. Statistical differences were noted among the three classes (P < 0.05). For example, there was a statistical difference in the 2015 and 2016 classes, compared with the 2014 class (P < 0.017), and all had statistical differences between the classes of 2015 and 2016 (P < 0.017). After the effective construction of medical record-related questions in the heuristic teaching of medical records, students’ understanding and mastery of knowledge points were enhanced, and the quality of teaching has improved. However, the design of medical record-related issues still needs to be optimized, in order to achieve effective classroom questioning.

BACKGROUND:The inflammatory skin wound response is regulated by argonaute 2-bound microRNAs (Ago2-miRNAs) such as miR-139-5p, which inhibit transcription of their target mRNAs. Jiang Tang Xiao Ke (JTXK) is a traditional Chinese medicine that reduces miR-139-5p expression, suggesting that topical application of JTXK may have effects on wound healing.METHODS:miR-139-/- mice and wild-type (WT) mice were employed to characterize the in vivo effects of miR-139-5p on sterile wound healing. Neutrophil migration and activation into the wound site were examined by live imaging analysis in lys-EGFP mice and myeloperoxidase/aminophenyl fluorescein assays, respectively. In silico and in vitro studies in differentiated HL60 cells were performed to identify miR-139-5p's downstream mediator(s). miR-139-/- neutrophil transplantation (with or without Eif4g2-knockdown rescue) or a topical JTXK gel preparation (with or without miR-139-5p mimic rescue) were employed to characterize the in vivo effects of miR-139-5p and JTXK, respectively, on Staphylococcus aureus (S. aureus)-infected wound healing.RESULTS:miR-139-/- mice display impaired sterile wound healing but improved S. aureus-infected wound healing. Eif4g2, a protein that supports neutrophil proliferation and differentiation, was identified as a key downstream mediator of miR-139-5p. miR-139-/- mice show elevated neutrophilic activation and Eif4g2 upregulation. miR-139-/- neutrophils enhanced S. aureus-infected wound healing in an Eif4g2-dependent manner. Moreover, topical JTXK gel therapy also enhanced S. aureus-infected wound healing in a miR-139-5p-dependent manner.CONCLUSIONS:miR-139-5p negatively regulates the neutrophilic response during S. aureus-infected wound healing, suggesting that JTXK or other miR-139-5p suppressants may be effective for treating infected skin wounds.