The expression of BADH gene of wheat seedlings was markedly lowered by cycloheximide and antinomycin D, while the expression was increased by chloramphenicol, showing the expression of the BADH gene was regulated and controlled at transcription level and translation level. The expression could be also regulated by the repressor synthesized in the mitochondria. The expression of BADH gene was lowered by H 7 and mannose. Reciprocally, the expression of BADH gene was markedly increased by Okadaic acid, showing the probable importance of protein phosphorylation in the expression in the BADH gene in wheat seedlings.
KCN (2 mol/L), antimycin A (2.7 μmol/L), NaN3 (6 mmol/L) 和SHAM (salicylhydroxamic acid, 5 mmol/L) 分别降低小麦芽中的BADH基因表达约40%,41%,46% 和22.0%, 而ATP, ADP 分别增加BADH基因表达约33% 和34%,DNP (2,4-dinitrophenol, 1 mmol/L) 降低BADH基因表达约30%。 这些结果表明,正常运转的末端氧化途径所形成的高能化合物对BADH基因表达是重要和必需的,细胞色素途径比交替途径对BADH基因表达有更大的影响。这是首次对“多条路线”假说关于呼吸代谢调节植物基因表达的观点提供直接的证据。
转甜菜碱醛脱氢酶(BADH)基因烟草叶片中超氧物歧化酶(SOD)的活性比对照增加约36%,过氧化氢酶(Cat)和过氧化物酶(POD)活性分别提高约62%和88%,定位于叶绿体中的抗坏血酸-谷胱甘肽途径的抗坏血酸过氧化物酶(AsAPOD),脱氢抗坏血酸还原酶(DAsAR)和谷胱甘肽还原酶(GR)活性分别提高67.7%,47.9%和38.8%,表明由SOD催化阴离子自由基O2所产生的活性氧H2O2能被完全分解,不会衍生毒性很强的羟自由基·OH.定量说明两类抗氧化酶分解自由基与活性氧的相互匹配,转基因植株的耐光抑制能力比对照提高幅度较大,这可能是由于抗氧化酶活性提高,更有效清除了活性氧和自由基,保护了叶绿体的结构与功能,证明抗氧化酶活性的提高可能是转BADH基因植株抗性提高的重要原因之一.