Objective:Screening the causative genes of nonspecific interstitial pneumonia (NSIP) by bioinformatics and provide targets for further research.Method:By downloading the gene chip datasets GSE110147, GSE21369, GSE40839 from the GEO database, and using the limma package analysis tool to screen out the differentially expressed genes between normal tissues and NSIP. The clusterProfiler package was used to perform GO analysis and KEGG pathway enrichment analysis on the differentially expressed genes to find the biological functions of the differentially expressed genes and their concentrated signaling pathways in the pathogenesis of NSIP. To study the relationship between differentially expressed genes and proteins, the STRING database and CYTOSCAPE software were used to construct the protein-protein interaction (PPI) network, and the MCODE software was used to extract the sub-network modules in the protein interaction network.Result:Veen plots result suggested three common significantly differentially expressed genes were found. GO enrichment analysis showed that up-regulated differentially expressed genes in NSIP mainly affected biological processes related to RNA splicing, antiviral infection, and cellular responses to peptides. The enriched molecules are mainly involved in the synthesis, secretion, and splicing complexes of cellular components, and the enriched molecular function are mainly involved in ATPase activity, receptor ligand activity and DNA-binding transcription activator activity. The down-regulated proteins in NSIP are mainly involved in biological processes regulated by transferase activity. KEGG pathway analysis showed that the up-regulated differentially expressed genes in NSIP were mainly involved in signaling pathways such as PI3K-Akt pathways, human papilloma virus infection pathways and MAPK pathways.Conclusion:The differentially expressed genes screened by bioinformatics may be new targets for the pathogenesis of NSIP, which is significant for the future clinical diagnosis and treatment of NSIP.

实验动物是指用在科学研究、教学、生产和检定当中的动物,对人类健康、医疗事业的进步有巨大贡献.为适应国家对于医学创新型、实践型人才的需求,医学院校逐渐落实了医学实验动物学课程,旨在提高医学生的实践操作动手能力.医学院校作为培养医学生的主要阵地,在积极展开实验动物血课程的同时,为了保证动物实验开展的科学、合乎于道德,还应重视对学生的动物伦理学教育.使医学生能规范实验行为,培养其正确的医学伦理观,实现医学教育与素质教育的融合.