During this phytochemical study, 13 compounds from the bark of Salix cinerea L. were isolated and their structures elucidated. These included two salicylic alcohol derivatives, one flavonol, two phenylpropanoids, two flavan-3-ols, two dimeric procyanidins, two dimeric prodelphinidins, and a unique ester of catechin (3-O-(1-hydroxy-6-oxo-2-cyclohexen-1-carboxylic acid), HCH-catechin). Furthermore, seasonal variations in the composition of Salix cortex regarding proanthocyanidins (PA) and the degree of polymerization were examined using NMR spectroscopy, revealing an increase in polymerization throughout the growing season 2020 associated with a consistent hydroxylation pattern in the B-ring. The isolated HCH-catechin was tested in vitro for its inhibitory effect on TNF-α-induced ICAM-1 expression in human microvascular endothelial cells (HMEC-1). A 24 h treatment with a 25 µM solution of HCH-catechin significantly reduced ICAM-1 expression (83.7 ± 3.2%) compared to unsubstituted catechin (97.9 ± 4.4%). Additionally, during a mass-spectrometric screening, numerous HCH adducts within the PA fraction could be identified, allowing for the proposition of a characteristic fragmentation pattern. This study establishes a foundation for a comprehensive assessment of the phenolic, PA-rich fraction in willow bark, particularly the occurrence of HCH adducts, which may contribute to the medicinal properties of Salicis cortex. Findings on seasonal variations and mass spectrometric profiling offer new insights into the quality standards for Salicis cortex as a medicinal remedy.

The abundant use of commercial anti-infective agents has led to the resistance development in bacterial strains, the presence of their residues in animal products and other negative consequences. The aim of the present work was to examine the antibacterial activity of EOs obtained from three plants (Satureja montana L., Ocimum basilicum L. and Salvia officinalis L.) against field isolates and reference bacterial strains of the most important pathogens in the cow endometrium. Their susceptibility was tested in vitro using a disk diffusion method, and the agar dilution test (ADT) and microdilution test (MDT) were used to determine the minimum inhibitory concentrations (MIC). In addition, the cytotoxic effect of the highest effective oil was tested by MTT, using the two cell lines, i.e. the BEND cell line (endometrium, 24h after exposure), and the MDBK cell line (kidney, 3, 6, 12 and 24h after exposure). The results of the antimicrobial assays suggest that the EO of S. montana, consisting mainly of p-cymene (42.8%) and carvacrol (28.1%), is a potent antimicrobial agent with high zones of inhibition determined on the disk diffusion method, where none of the individual isolates were resistant. Moreover, the calculated MIC values on both ADT and MDT were <1 mg/mL for bacterial isolates of all species. In addition, the oil exhibited low cytotoxic potential on both BEND (IC50=1.27 mg/mL) and MDBK cell lines (IC50=1.02-1.56 mg/mL). The obtained results indicate the possibility of the use of S. montana EO for the treatment of bacterial-induced reproductive diseases in cows.

This study aimed to assess the photocatalytic removal efficiency of the fluoroquinolone antibiotic ciprofloxacin (CIP), a pharmaceutically active compound of Ciprocinal (R), from the aquatic environment. It focused on evaluating how various influencing factors - radiation type, catalyst loading, initial substrate concentration, CIP source, the introduction of different electron acceptors and scavengers of reactive species, water matrix, as well as photoreactor design - influence the photodegradation efficiency of the antibiotic. Namely, CIP photolytic removal efficiency was enhanced in the presence of H2O2, (NH4)(2)S2O8, and KBrO3. The highest reaction rate was achieved with a ZnO loading of 1.0 mg/mL, while the degradation rate of CIP increased with the initial concentration across the studied concentration range (0.0025-0.05 mmol/L). Molecular O-2 has the best electron acceptor properties in CIP photocatalytic experiments, whereas central role in the degradation mechanism belongs to HOads center dot, h(+), and O-2(center dot-). Next, CIP present in a commercial formulation was more prone to removal than the analytical standard, and its photocatalytic degradation occurs in natural water, however with a reduced removal efficiency compared to ultrapure water. Moreover, reaction intermediates formed during photocatalytic degradation were studied in detail by employing LC-ESI-MS2 technique, whereby the formation of nine degradation intermediates was revealed, and possible CIP degradation patways were proposed. Lastly, ECOSAR model predicted that these intermediates do not pose acute or chronic toxicity towards the aquatic organisms.

BACKGROUND:The increasing difficulties in combating anthelmintic resistance in gastrointestinal nematodes (GINs) of sheep worldwide, and the residues of chemical drugs in animal products and the environment, necessitate the search for alternatives. Previous studies have shown that plant essential oils (EOs) could be valuable anthelmintic agents, due to their numerous advantages. The present study aimed to evaluate the possibility of using winter savory (Satureja montana L.) EO against sheep GINs. The chemical composition of the tested oil was determined by gas chromatography-mass spectrometry (GC-MS). The efficacy of the tested oil was determined in vitro using the egg hatch test (EHT), and in vivo using the faecal egg count reduction test (FECRT) performed in two farms. Preliminary toxicity studies including clinical observation, haematological and biochemical blood analysis were also performed to evaluate the safety of the tested oil to the hosts. In addition, a coproculture study was carried out in the tested farms using the appropriate morphological keys. RESULTS:Main compounds of the S. montana oil identified by GC-MS analysis were p-cymene (42.8%), carvacrol (28.1%) and y-terpinene (14.6%). The in vitro EHT showed a dose-dependent (R2 = 0.94) anthelmintic potential of the tested oil, with ovicidal activity varying from 17.0-83.3% and determined IC50 value of 0.59 mg/ml. The field efficacy reached 33% (at group level) and 50% (at individual level) at D14 after treatment. In vivo efficacy was significantly higher in farm 2 (FEC above 65% at group level, p < 0.05) where sheep were kept in pens during treatment. No toxic effects were observed, either in the physical observation of the test animals or in their liver and kidney function. No significant changes (p > 0.05) in the percentage representation of GIN genera were observed in the coproculture study, indicating that the treatment agent was not specific to a single genus. CONCLUSION:The anthelmintic potential showed on EHT and FECRT, without adverse effects on the sheep, suggests that S. montana EO is suitable for the control of sheep GINs as part of an integrated parasite management. However, further studies should be conducted to increase efficacy in field conditions.

Crataegus species (hawthorn) have been commonly used in traditional medicine, especially for the treatment of congestive heart failure. Many studies confirmed that they are rich in polyphenols, thus exhibiting strong antioxidant activity, which contribute to the beneficial effects of hawthorn on the cardiovascular system. In the market, there are many herbal medicinal products based on hawthorn, which consumption as adjuvant therapy in heart-related issues is supported by European Medicines Agency. Since there is a global trend of making homemade herbal preparations, this study aimed to compare whether there is a difference in polyphenol profile and antioxidant potential between homemade and commercial ethanol extracts of hawthorn. Polyphenol profile was evaluated by determination of total phenolic and flavonoid contents, and by quantitative analysis of selected polyphenols by liquid chromatography? mass spectrometry/mass spectrometry. Antioxidant potential was examined by 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing antioxidant power and lipid peroxidation inhibition assays. The results of this study suggest that homemade ethanol extracts of hawthorn flowers, leaves and fruits are just as good source of polyphenols and antioxidants as commercial ones, and their utilization should be supported. Furthermore, hawthorn extracts made of leaves and flowers are better source of bioactive polyphenols and have higher antioxidant activity compared with the same of fruits, regardless of the method of preparation.

Even though we have only one planet to live on, environmental pollution is still an urgent global issue. Among all-natural threatened resources, the drinkable water has one of the most burdening influences on the life quality and economy. The pharmaceutically active ingredients are the most concerning pollutants. In this study, heterogeneous photocatalysis was used as a sustainable alternative for tolperisone hydrochloride (TLP) removal from water. The degradation pathway of TLP was also analyzed using various scavengers and it was found that the contribution of reactive species changed in following order: OHfree > h(+) >> OHads center dot> e(-). Furthermore, 12 photodegradation intermediates of TLP were identified using LC-ESI-MS/MS. In addition, the toxicity of TLP and its degradation intermediates were examined on barley, using chemometrics. The most significant variations were observed on barley plant after 4 days of germination and on the root, during the barley biomass production. The results also showed that TLP and its intermediates are harmful to the plants, due to deteriorating water uptake with toxic TLP-compounds that reach the vital parts of plants. Also, slightly variations in total nitrogen amount were observed. The high genetic variability efficiently guards barley from various environmental stresses.

Wine represents a complex matrix of polyphenolic compounds. Polyphenols alter the sensory qualities of the wine, represent chemotaxonomic markers and are responsible for many of its health benefits. It is critical to accurately quantify these compounds, which are the subject of extensive current research. Quantification usually requires HPLC analysis, where the main step in sample preparation involves filtration. With the assumption that sample compounds remain on the syringe filter, this study investigated whether diluting the sample before filtration would enable a better recovery of polyphenols, yielding more accurate results. For this purpose, a red, rosé and white wine were diluted with 4 different solvents (water, methanol, ethanol and a 1:1 methanol-ethanol mixture) prior to filtration, and their polyphenolic composition was analyzed. Only 7 polyphenols present in low concentrations were not affected by dilution, while 38 compounds were all detected in higher concentrations when applying certain solvents, compared to undiluted wine. The methanol-ethanol mixture enabled the best flow of polyphenolics through the filter, while the water dilution showed that 30-40% of the sum of anthocyanins remained on the filter if a dilution step was not included in the sample preparation procedure. This study confirmed the need to incorporate a preliminary evaluation of the filtration step during sample preparation to improve the accuracy of subsequent HPLC analyses.

Anthriscus sylvestris (L.) Hoffm. (Apiaceae), commonly known as wild chervil, has gained scientific interest owing to its diverse phytochemical profile and potential therapeutic applications. The plant, despite being categorized as a noxious weed, is traditionally used in treating various conditions like headaches, dressing wounds, and as a tonic, antitussive, antipyretic, analgesic, and diuretic. Its pharmacological importance stems from containing diverse bioactive lignans, especially aryltetralins and dibenzylbutyrolactones. One of the main compounds of A. sylvestris, deoxypodophyllotoxin, among its wide-ranging effects, including antitumor, antiproliferative, antiplatelet aggregation, antiviral, anti-inflammatory, and insecticidal properties, serves as a pivotal precursor to epipodophyllotoxin, crucial in the semisynthesis of cytostatic agents like etoposide and teniposide. The main starting compound for these anticancer medicines was podophyllotoxin, intensively isolated from Sinopodophyllum hexandrum, now listed as an endangered species due to overexploitation. Since new species are being investigated as potential sources, A. sylvestris emerges as a highly promising candidate owing to its abundant lignan content. This review summarizes the current knowledge on A. sylvestris, investigating its biological and morphological characteristics, and pharmacological properties. Emphasizing the biological activities and structure–activity relationship, this review underscores its therapeutic potential, thus encouraging further exploration and utilization of this valuable plant resource.

Cabernet Sauvignon grape juice and wine underwent in vitro digestion, resulting in a reduction of most phenolic compounds (10% - 100% decline), notably impacting anthocyanins (82% - 100% decline) due to pH variations. However, specific phenolics, including p - hydroxybenzoic, protocatechuic, vanillic, p -coumaric, gallic and syringic acids, and coumarin esculetin, increased in concentration (10% - 120%). Grape juice and wine samples showed comparable polyphenolic profile during all phases of digestion. Antioxidant activity persisted, and inhibition of angiotensin-I converting enzyme was improved after the digestion process, likely because of increased concentrations of listed phenolic acids and esculetin. Digested grape juice displayed comparable or superior bioactivity to red wine, indicating it as a promising source of accessible grape polyphenols for a broader audience. Nevertheless, Caco-2 cell model metabolization experiments revealed that only 3 of 42 analyzed compounds passed to the basolateral compartment, emphasizing the significant impact of digestion on polyphenol bioactivity, suggesting potential yet unmeasurable and overlooked implications for human health.

Gastrointestinal nematodes (GINs) represent major obstacle to modern sheep farming, where the exclusive use of commercial anthelmintics is no longer a sustainable option due to the development of resistance in nematodes and the problem of drug residues in animal products and the environment. The aim of this study was to investigate the in vitro phytotherapeutic properties of seven essential oils (EOs) against GINs in sheep. Their chemical composition was determined by gas chromatography-mass spectrometry (GC-MS) analysis. The anthelmintic potential was evaluated using the egg hatch test performed at eight concentrations for each oil (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL). Additionally, a coproculture examination was performed to identify the GIN genera present in the tested fecal samples. The tested EOs showed a dose-dependent (R2 close to 1) ovicidal activity with basil- Ocimum basilicum L. (23.3-93.3%, IC50=0.08 mg/mL), spearmint- Mentha spicata L. (13.0-92.7%, IC50=0.07 mg/mL) and hyssop- Hyssopus officinalis L. (42.7-91.3%, IC50=0.19 mg/mL) being the most effective. The high activity of these oils could be attributed to the high content of the monoterpene alcohol linalool (O. basilicum) and the terpenoid ketones carvone (M. spicata) and pinocamphone (H. officinalis). Four genera of sheep GINs were identified on coproculture examination, i.e. Haemonchus 43%, Trichostrongylus 35%, Teladorsagia 17% and Chabertia 5%. The obtained results suggest that EOs of basil, spearmint and hyssop could represent a valuable alternative in the control of GINs in sheep, which could help to reduce the use of chemical drugs. Future field trials should be performed to confirm these findings.

This paper presents a comprehensive study of commercially available monovarietal red and white wines from Fruška Gora mountain in Serbia, conducted to characterize their chemical and biochemical properties. Namely, 72 wines of 18 different Vitis vinifera varieties, including international, domestic and autochthonous ones, were assessed. Phenolic profile of wines was determined qualitatively, using spectrophotometric methods, and quantitatively, using LC-MS/MS and HPLC-UV/VIS methods. Quantified phenolic compounds pointed out differences between red and white wines, where some compounds stood out as distinctive characteristic of analyzed varieties, while others served as chemotaxonomic markers of the Fruška Gora region. Antioxidant and inhibitory properties towards acetylcholinesterase were evaluated for all wines and their relation to phenolic composition was investigated. This study is valuable since chemical and biochemical properties of domestic and autochthonous Fruška Gora varieties are reported for the first time. Also, international varieties studied herein gave distinctive information about the terroir of Fruška Gora region, which is scarce in scientific literature.

Background Gastrointestinal nematodes (GINs) represent major obstacle to modern small ruminant farming due to their various negative effects on animal health, welfare and productivity. The exclusive use of commercial, synthetic anthelmintics is no longer a sustainable option due to the development of resistance in GINs and the problem of residues of these drugs in animal products and the environment. The aim of this study was to investigate in vitro the phytotherapeutic properties of seven different plant essential oils (EOs): Citrus x sinensis (L.) Osbeck, Citrus medica (L.), Mentha spicata (L.), Ocimum basilicum (L.), Hyssopus officinalis (L.), Salvia officinalis (L.) and Lavandula angustifolia Mill. against GINs in sheep. Methods First, the chemical composition (qualitative and semi-quantitative characterization) of the tested EOs was determined by gas chromatography–mass spectrometry (GC–MS) analysis. Their anthelmintic potential was evaluated in vitro using the egg hatch test, which was performed at eight different concentrations of each oil: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml. Finally, a coproculture examination was performed to determine the genera of GIN against which the oils were tested. Results Some of the most frequently identified compounds in GC-MS analyses were limonene, myrcene, α- and β- pinene, γ-terpinene, sabinene, linalool, eucalyptol, terpinen-4-ol, camphor, etc., with their total number per oil varying from 8 (C. sinensis) to 42 (O. basilicum). The tested EOs showed a dose-dependent (R2 close to 1) ovicidal activity, with O. basilicum (23.3–93.3%, IC50 = 0.08 mg/ml), M. spicata (13.0-92.7%, IC50 = 0.07 mg/ml) and H. officinalis (42.7–91.3%, IC50 = 0.19 mg/ml) being the most effective. The high activity of these oils could be attributed to the high content of the monoterpene alcohol linalool and the terpenoid ketones carvone and pinocamphone. The least effective were S. officinalis (15.0–89.0%, IC50 = 0.53 mg/ml) and C. medica 12.3–95.0% (IC50 = 2.52 mg/ml). Four genera of sheep GINs were identified on coproculture examination, i.e. Haemonchus 43%, Trichostrongylus 35%, Teladorsagia 17% and Chabertia 5%. Conclusion The results obtained suggest that EOs tested, especially wild mint, basil and hyssop, could represent a valuable alternative in the control of GINs in sheep, which could help to reduce the use of chemical drugs. Future field trials should be performed to confirm these findings.

Steaming of green timber, a common industrial process for various hardwood species, significantly influences wood properties, including coloration and drying characteristics. However, the environmental implications of substantial volumes of condensate generated during wood steaming underscore the urgency for its sustainable management. This study explores the chemical composition of the condensate obtained during the 90-hour indirect steaming of walnut timber (WTSC), aiming to identify potential applications for this wastewater while addressing environmental risks. Chemical characterization of WTSC included qualitative LC-MS/MS analysis, determination of the total phenolic content (TPC), total flavonoid content (TFC) and the content of selected phenolics. WTSC exhibited high TPC (188 mg gallic acid equivalents per L) and TFC (9.74 mg quercetin equivalents per L) values. Additionally, WTSC showed significant antioxidant activity (IC50 (DPPH) = 61.4 µg/mL and 103 µg ascorbic acid equivalents per mL in FRAP assay). Specific phenolic compounds detected in the WTSC distinguish it from other wood industry effluents and are a consequence of the unique characteristics of walnut wood and conditions during steaming process. A variety of acids (p-hydroxybenzoic, protocatechuic, syringic, gallic, cinnamic, cinnamic, p-coumaric, o-coumaric, vanillic) and flavonoids (apigenin, genistein, naringenin, luteolin, kaempferol, chrysoeriol, isorhamnetin, apigenin 7-O-glucoside, vitexin, kaempferol 3-O-glucoside, catechin, epicatechin, and quercitrin) were identified and quantified. The condensate exhibited higher TPC value and antioxidant activity than other wood industry effluents, positioning it as a promising natural antioxidant with potential applications in pharmaceutical and food industries. However, our short-term goal is to explore the potential use of WTSC as received – without isolating individual compounds – in studies focused on plant protection, textile dyeing, and wood-based panel production.

Intensification of poultry production has been associated with an increase in parasite prevalence and adaptation of an invasive external parasitic species, such as the poultry red mite (Dermanyssus gallinae). The studies of biological efficacy (contact and fumigant) and level of toxicity to mites indicated that the external application of essential oils (EOs) can be an alternative to acaricides. In this study, the results of acaricidal efficacy of eight selected EOs - Lavandula angustifolia Mill., Laurus nobilus L., Mentha x piperita L., Mentha spicata L., Ocimum basilicum L., Salvia officinalis L., Satureja montana L. and Thymus vulgaris L. are presented. Their chemical profiles were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The acaricidal efficacy of EOs (6% concentration) was tested on adult mites over 10 days in laboratory conditions using the Petri-dish method, through direct exposure for 1 min (contact toxicity) and subsequent exposure for 1 h (residual toxicity). The most effective EO in direct exposure-contact, after 48 h of observation, was S. montana (100% toxicity), while the greatest residual effect was observed in T. vulgaris (11% toxicity). The obtained results showed high efficacy of the EOs against the mites through direct contact and thus their great acaricidal potential. However, the activity was lost with subsequent exposure, indicating the absence of prolonged effect. Possible ways to overcome this problem are discussed further in this paper. In any case, the present study confirmed the acaricidal potential of herbal medicines that can be used in the integrated control of poultry red mite.

Background: The increasing difficulties in combating anthelmintic resistance in gastrointestinal nematodes (GINs) of sheep worldwide and the residues of chemical drugs in animal products and the environment necessitate the search for alternatives. Previous studies have shown that plant essential oils (EOs) could be valuable anthelmintic agents. The aim of this study was to evaluate the possibility of using winter savory (Satureja montana L.) EO against sheep GINs through performing in vitro and in vivo efficacy tests and preliminary toxicity studies. Methods: The chemical composition of the S. montana EO was determined by gas chromatography-mass spectrometry (GC-MS). For the evaluation of anthelmintic potential in vitro, the Egg Hatch Test (EHT) was performed at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml). Field efficacy was evaluated using the Faecal Egg Count Reduction Test (FECRT) on two farms where the oil was applied intraruminally to sheep at a mean dose of 150 mg/kg. The toxic effect of the oil was evaluated by physical observation of the tested animals and by collecting blood samples for further haematological and biochemical analyses. Coproculture examination of faecal samples from the tested sheep before and after treatment was also performed to identify the GIN genera present. Results: Main compounds of the S. montana oil identified by GC-MS analysis were p-cymene (42.8%), carvacrol (28.1%) and y-terpinene (14.6%). The in vitro EHT showed a dose-dependent (R2=0.94) anthelmintic potential of the tested oil with ovicidal activity varying from 17.0-83.3% and determined IC50 value of 0.59 mg/ml. The field efficacy reached 33% (at group level) and 50% (at individual level) at D14 after treatment. In vivo efficacy was significantly higher in farm 2 (FEC above 65% at group level, p<0.05) where sheep were kept in pens during treatment. No toxic effects were observed either in the physical observation of the test animals or in their liver and kidney function. Conclusion: The moderate efficacy on EHT and FECRT, without adverse effects on the sheep, suggests that S. montana EO is suitable for the control of sheep GINs as part of an integrated parasite management. Further studies should be conducted to further increase efficacy.

The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.

Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0-90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography-mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.

Wild chervil (Anthriscus sylvestris (L.) Hoffm.), known also as cow parsley, is a widely distributed herbaceous plant from Apiaceae family. Traditional use as analgesic, antipyretic, antitussive, tonic, diuretic etc. is associated with the proven presence of various types of biomolecules, primarily lignans that possess cytotoxic, antiproliferative, insecticidal, antiviral and other activities. The majority of lignans reported in A. sylvestris belong to two classes - aryltetralins and dibenzylbutyrolactones. The data of lignan profile of A. sylvestris are mostly limited to root and herb composition, therefore LC-DAD-ESI-MS/MS analysis was used for the tentative identification of 15 compounds from the fruit extract of A. sylvestris, of which two were never reported in nature.