Objective To study the delayed protective effects of limb ischemia preconditioning(LIPC) on liver ischemia-reperfusion(I-R) injury in rats.Methods Thirty-six SD rats were equally randomized into there groups of S(sham operated),I-R(I-R model) and LIPC(LIPC before I-R).I-R model was established by 70% liver ischemia for 1 hour and reperfusion for 3 hours.LIPC was performed by 5-min ischemia of two posterial limbs for three times.Blood and liver samples were obtained to determine the activity of total superoxide dismutase(tSOD),malondialdehyde(MDA),ALT and AST,liver wet/dry weight(W/D),and the expression of TNF-α.The ultrastructural damage of the liver was observed as well.Results Compared with group I-R,the activities of MDA,ALT and AST were significantly decreased,tSOD level was increased and W/D and the expression of TNF-α were obviously reduced in group LIPC(P0.01).The damage of liver tissue was significantly less in group LIPC than that in group I-R.Conclusion LIPC has an delayed protective effect on liver I-R injury in rats,which is possibly related to an increase of the antioxidant capacity,suppression of liver inflammatory reaction,inhibition of the expression of TNF-α and improvement of liver microcirculation.

目的应用几何分割法改进手术设计,用扩张皮瓣修复瘢痕性秃发及面部皮肤缺损。方法运用AutoCAD软件及缺损创面几何分割法改进术前设计,用扩张器精确修复30例瘢痕性秃发及先天性畸形继发的面部缺损整形的患者。结果 30例患者扩张皮瓣均全部成活,修复后形态自然,随访6个月-1年,效果满意。结论通过改进术前设计,可以使得扩张皮瓣修复瘢痕性秃发及面部皮肤缺损变得精确、简单易行。

目的 通过对微创小切口法治疗腋臭手术过程中病理组织学的动态研究,探讨一种判定达到根治腋臭的临床标准.方法 对30例腋臭患者进行搔刮手术,根据以往的临床经验确立的标准及时停止手术,并对手术开始、进行、结束时选取4个时间点采集标本,进行病理组织学分析及疗效判定.结果 30例患者都达到了根治效果,随访6个月～1年,效果满意,无复发.病理学检查结果显示标本1有少量的大汗腺组织,标本2、3可见大量发达的大汗腺组织,标本4仅有极少量的大汗腺组织.结论 根据我们确立的微创法治疗腋臭过程中手术深度的临床判定标准,能够达到根治的目的,值得进一步研究和推广.

间充质干细胞来源广泛,体外扩增能力强,具有多向分化潜能,是组织工程研究中重要的种子细胞和基因载体,具有极大的研究和应用价值。同时在研究中还发现它具有免疫原性弱,不诱导免疫排斥反应等特点,但对于机制尚不十分清楚。本文将对间充质干细胞的免疫调节作用的研究现状作一简要综述。