Addressing environmental degradation and fostering agricultural sustainability are critical challenges of the twenty-first century. Agroecological practices such as organic and biodynamic farming are vital for transforming agriculture and enhancing ecosystem services by avoiding synthetic inputs. While often criticized for lower yields, little is known about how these systems adapt and perform over extended periods under varying climatic conditions, particularly for perennial crops such as grapevines. This represents a major knowledge gap, as understanding their long-term adaptive capacity is essential for designing resilient and sustainable production systems under climate change. This 18-year field trial in Geisenheim, Germany, addresses this gap by assessing the long-term effects of organic, biodynamic, and integrated management on Vitis vinifera cv. Riesling. It represents the only long-term study on perennial crops to systematically compare these systems’ impact on agronomic parameters, plant performance, and grape quality, observing their evolution post-conversion and response to climatic variability. Initially, organic (−17

The potential of soils to maintain biological productivity, defined as soil health, is strongly influenced by human activity, such as agriculture. Therefore, soil management has always been a concern for sustainable agriculture and new methods that account for both soil health and crop yield must be found. Biofertilization using microbial inoculants emerges as a promising alternative to conventional interventions such as excessive mineral fertilization and herbicide use. Biodynamic preparations used as a central part of biodynamic agriculture have various effects on soil properties, such as microbial biomass and respiration. We conducted several biomarker experiments to infer the effect of biodynamic preparations on soil prokaryotic and fungal communities and compared results to organic management. Potential plant growth promoting amplicon sequence variants were quantified using a commercial database based on their taxonomic identity. We found significantly higher numbers of putative plant growth promoting amplicon sequence variants in biodynamically compared with organically treated soils. Furthermore, prokaryotic amplicon sequence variants enriched in biodynamic preparations were found in higher numbers in biodynamically treated soils, indicating successful colonization after treatment. Experiments were conducted at three locations in Germany and 21 locations in France covering different crops and soil types. Altogether, our results indicate that biodynamic preparations can act as biofertilizers that promote soil health by increasing the abundance of plant growth promoting microorganisms.

The image-forming methods copper chloride crystallization, capillary dynamolysis and circular chromatography are presented as an instrument for assessing wine quality. Wine quality of samples from a long-term field trial comparing integrated, organic and biodynamic management were investigated by using image-forming methods and sensory analysis. Concerning the image-forming methods copper chloride crystallization, capillary dynamolysis and circular chromatography, the images of encoded samples were (i) grouped into pairs with similar image features; (ii) characterized based on reference images (e. g. high–low resistance to degradation); (iii) ranked (according to the characterization), and (iv) assigned to the different production methods (classified). Wine samples from organic and biodynamic management needed less wine per sample for a similar expression of structural characteristics than wine samples from integrated cultivation. Organic and biodynamic samples also show structures that indicate less degeneration than integrated samples. Due to these properties, nine coded wine samples from 2010 could be (i) grouped, (ii) characterized, (iii) ranked and (iv) classified without errors, i.e., assigned to the cultivation methods of integrated, organic and biodynamic agriculture. In sensory analysis, the wine derived from biodynamic management had the highest aroma intensity. In the other parameters the differences were not significant. Analysis with the image-forming methods copper chloride crystallization, capillary dynamolysis and circular chromatography complements sensory analysis for a more complete description of the characteristic properties of wines originating from different management systems. If further studies confirm these results, the image-forming methods copper chloride crystallization, capillary dynamolysis and circular chromatography may be developed as a complementary tool to sensory and chemical analysis in assessing wine quality.

Aim: The image forming methods biocrystallisation, capillary dynamolysis and circular chromatography are introduced as a complementary tool for grape quality assessment. These methods were used to investigate grape juice samples from a long-term field trial comparing integrated, organic and biodynamic viticultural practices.Methods and results: Characteristic changes in structures created by the reaction of metal salts with grape juice were evaluated using biocrystallisation, circular chromatography and capillary dynamolysis image forming methods. In particular, this study tested the effects of cultivation method, aging time and juice concentration on structure formation.To assess grape quality, the images of the encoded grape juice samples were: i) grouped into pairs with similar image features, ii) characterised based on reference images (e.g., high versus low resistance to degradation, or the amount of substance necessary for structure formation), iii) ranked according to structures associated with grape quality, and iv) assigned to the different production methods (classification). In order for similar structural features in the image forming methods to be expressed, all samples of grape juice harvested from integrated production over four years required higher juice concentrations than samples from organic and biodynamic origin. This was interpreted as the latter two production systems having higher structure formation efficacy. Furthermore, juices produced from the integrated management system exhibited more structures, indicative of a lower resistance to aging. In three out of four harvest years, the biodynamic samples exhibited the highest structure forming efficacy and resistance to aging.Conclusion: These findings are consistent with enhanced form maintenance and thus higher internal product quality of biodynamic and organic grapes compared to grapes from integrated farming.Significance and impact of the study: Image forming methods may serve as a valuable tool for grape juice and wine quality assessment to complement compound-specific chemical analyses.

Aim: The effects of integrated, organic and biodynamic management on soil quality and the growth and morphological development of Riesling grapevines were assessed during the first 4 years of a long-term field trial in Geisenheim, Germany. The overall aim was to understand the effects of these different viticultural practices on soil quality and plant morphology as the basis for product quality. Methods and results: As indicators of soil quality, earthworm abundance and the activity of selected enzymes were assessed. The vegetative and reproductive development of the grapevines, as well as their susceptibility to fungal diseases in the field, wood and grape composition, and grape yield, were investigated. Individual variables were subjected to analysis of variance. Additionally, all variables were subjected to multivariate principal component analysis. Compared with plots under integrated management, plots under the two biological treatments were characterized by higher soil quality and lower vegetative growth and grape yield, and therefore higher exposure of grapes and lower grape cluster compactness, and, probably as a result of these morphological differences, lower incidence of acetic acid rot. Principal component analysis clearly differentiated the three treatments, and showed that biodynamic management had more pronounced effects than organic management in terms of enhanced soil fertility and reduction of vegetative growth. Conclusions: In the present study, organic and especially biodynamic management resulted in a morphology favouring production of high-quality grapes. The treatments differed in terms of fertilization and plant protection methods as well as choice of cover crops. Therefore, further research is necessary with respect to root growth and the nitrogen and water uptake dynamics of vines and cover crops. The differences between grapes produced under organic and biodynamic management emphasize the need for more research on the mode of action of biodynamic preparations. Significance and impact of the study: In recent years, both winegrowers and consumers have expressed steadily growing interest in organic and especially biodynamic wine production. The present study contributes to a better understanding of the effects on grapevine growth and morphological development of shifting to these methods as a way to increase product quality.

In this study, the effects on grape juice quality resulting from the conversion of vineyard plots from integrated to organic and biodynamic management vs. integrated management were assessed using image forming methods. In addition, grape yield, Botrytis infection and the chemical composition of wood prunings and grapes were assessed. The images of replicate, encoded samples representing five different treatments were: (i) grouped into pairs displaying similar image features, (ii) characterised with respect to the quality domains 'strength of form expression' and 'resistance to deterioration' and (iii) ranked according to the quality characterisation. Based on the image analyses, all samples pairs were correctly grouped, i.e. the five production methods were precisely separated. The treatments were characterised as follows: (i) Integrated (INT): weakest form expression, most advanced deterioration; (ii) Conversion to Biodynamic without horn silica (BD0): strongest form expression, advanced deterioration; (iii) Conversion to Organic (ORG): weak form expression, low deterioration; (iv) Conversion to Biodynamic with 3 x horn silica (BD3): medium form expression, low deterioration; (v) Conversion to Biodynamic with 4 x horn silica (BD4): strong form expression, low deterioration. Principal component analysis (PCA) of field assessment and chemical analysis data revealed that the two treatments with the strongest form expression, BD0 and BD4, were characterised by high vigour, as indicated by high prune weight and primary amino nitrogen (NOPA). With respect to the resistance to deterioration, as depicted by image forming methods, no parallels were found with PCA.

In 2006 wurde ein weinbaulicher Feldversuch zum Vergleich verschiedener Bewirtschaftungssysteme (integriert, biologisch-organisch, biologisch-dynamisch) an der Hochschule Geisenheim University in Geisenheim, Rheingau, etabliert. Ertrag, Schnittholzgewicht und Zuckergehalt der Moste unterschied sich signifikant zwischen den Bewirtschaftungssystemen in einem Untersuchungszeitraum von 7 Jahren (2006-2012). Die integrierte Variante zeigte ein signifikant hoheres Schnittholzgewicht, einen signifikant hoheren Ertrag und signifikant niedrigeren Zuckergehalt des Mostes im Vergleich zu den beiden biologischen Systemen. Zwischen der biologisch-organischen und der biologisch-dynamischen Variante wurde kein Unterschied beobachtet. Weiter unterschieden sich die Bewirtschaftungssysteme weder im Gesamtsauregehalt noch im pH der Moste. Grunde fur das unterschiedliche Wachstum, den unterschiedlichen Ertrag und der unterschiedlichen Mostqualitat wie z.B. die Nahrstoff- und Wasserverfugbarkeit und die Haufigkeit auftretender Krankheiten in den unterschiedlichen Varianten mussen diskutiert werden. Ein Ruckgang von Schnittholzgewicht und Ertrag unter okologischer im Vergleich zu integrierter Bewirtschaftung wurde bereits in vorhergehenden Studien belegt. Allerdings sind die hier vorgestellten Untersuchungsergebnisse der erste Anhaltspunkt fur veranderte Zuckergehalte im Most und somit fur veranderte Produktqualitat durch unterschiedliche Bewirtschaftungssysteme im Weinbau.

Nine encoded wine samples from a German long-term field trial on the comparison of different cultivation systems were examined with the image forming methods biocrystallization, capillary dynamolysis and circular chromatography. The images of the encoded samples were i. differentiated into three groups of images with similar form expression, ii. characterised as ‘fresh – aged’ based on a catalogue of reference images, iii. ranked (according to the quality characterization) and iv. assigned to the different cultivation systems (classified). Images gained with samples from integrated farming showed more structures indicating enhanced degradation compared with the samples from organic and especially from biodynamic origin. Based on these observations, a correct assignment of all encoded samples to cultivation systems was possible. These results are interpreted as indicating higher product quality of biodynamic and organic wine compared to wine from integrated farming.

Based on a field trial, the impact of three different viticultural management strategies on vigour and grapevine physiology of Vitis vinifera cv. Riesling was compared. The vines were planted in 1991 at Geisenheim (Rheingau, Germany) and three different management strategies i.e. integrated (code of good practice), organic (European Union Regulation 834/07 and ECOVIN standard) and biodynamic (European Union Regulation 834/07 and DEMETER standard) were established in 2006. Even though all treatments received the same level of nutrients and water a decline in vigour, expressed as lateral growth, was observed for the organic and biodynamic treatment during three seasons (2010 to 2012). During dryer conditions (2011) a reduction of physiological activity expressed as stomatal conductance gs, assimilation rate A and transpiration E two weeks after full-bloom and a reduction in pre-dawn water potential at veraison were assessed for the biological treatments. In 2012 under wetter growing conditions neither differences in physiological activity nor in pre-dawn water potential were observed. Therefore changes in physiological activity and pre-dawn water potential are just partially responsible for the reduced vigour in the biological treatments.

World wide many wine farms are converting into organic or biodynamic viticulture. The main reasons are better wine quality and healthier wine with the long-term aim of sustainability in viticulture. In 2005 a long-term study started in Geisenheim. The objective of the research program is to investigate, compare and optimise the techniques of integrated, organic and particularly biodynamic wine production in terms of resource protection and food quality. The goals of this study are to look at the effects on the biological and microbial activity in the soil, the vegetative and generative growth of the vine, microbiology, the grape and wine quality and the sustainability of the three viticultural systems.

Nine encoded wine samples from a German long-term field trial on the comparison of different cultivation systems were examined with the image forming methods biocrystallization, capillary dynamolysis and circular chromatography. The images of the encoded samples were i. differentiated into three groups of images with similar form expression, ii. characterised as ‘fresh – aged’ based on a catalogue of reference images, iii. ranked (according to the quality characterization) and iv. assigned to the different cultivation systems (classified). Images gained with samples from integrated farming showed more structures indicating enhanced aging compared with the samples from organic and especially from biodynamic origin. Based on these observations, a correct assignment of all encoded samples to cultivation systems was possible. These results are interpreted as indicating higher product quality of biodynamic and organic wine compared to wine from integrated farming.