目的 观察3种不同方法处理下肢静脉性溃疡的临床疗效.方法 选择2019年1月至2021年1月收治的90例下肢静脉性溃疡的患者,按随机数字表法分为观察1组、观察2组和对照组,每组30例.依据伤口分期及患者的情况,观察1组根据伤口的分期选用不同种类的新型敷料,另加用生物活性敷料(外用重组人酸性成纤维细胞生长因子rhAFGF)换药,观察2组前期处理同观察1组,只是未加用生物活性敷料(rhAFGF)换药;对照组选用碘仿纱条和凡士林纱布换药.结果 观察1组伤口愈合总有效率明显优于对照组(P<0.01),观察1组优于观察2组(P<0.05);观察1组下肢静脉性溃疡愈合时间较对照组明显缩短(P<0.01),观察1组较观察2组缩短(P<0.01).结论 新型敷料联合生物活性敷料(rhAFGF)治疗下肢静脉性溃疡临床疗效显著,能有效缩短溃疡的愈合时间,值得临床应用.

目的 探讨心外膜脂肪体积(EATV)与冠状动脉粥样硬化性心脏病(冠心病)患者再发心血管事件的相关性,为临床治疗提供新的策略,为患者预后提供参考.方法 选取148例行冠状动脉(冠脉)CTA检查并冠脉造影术确诊冠心病患者,记录被试者基本临床资料及其它病史,通过前瞻性随访记录患者再发不良心血管事件的情况.根据EATV中位数128.02 cm3为界分为两组,低EATV组74例,高EATV组74例,比较两组间冠脉狭窄情况及再发不良心血管事件的相关性.结果 ①研究对象一般资料比较,性别、年龄、吸烟史、家族史、高血压病史、高密度脂蛋白、低密度脂蛋白、三酰甘油、总胆固醇水平方面差异均无统计学意义(P均>0.05);而糖尿病史、体质指数(BMI)在两组之间有显著性差异(χ2=3.918,P=0.048);BMI(t=-8.183,P=0.000),相关分析显示EATV与BMI呈正相关,相关系数r=0.590,P<0.01,而糖尿病与EATV间无显著相关性(r=-0.037,P>0.05).②高EATV组冠脉Gensini评分(50.98±19.55)明显高于低EATV组(35.01±17.24),两组间差异有统计学意义(t=-5.271,P=0.000),且EATV大小与冠脉病变严重程度呈正相关(r=0.418,P<0.01).③高EATV组冠心病再发不良心血管事件发生率明显高于低EATV组(χ2=13.669,P=0.000),Kaplan-Meier分析显示,高EATV组的生存时间低于低EATV组,且两组生存时间有显著差异(P<0.01).结论 EATV作为冠心病的独立危险因素之一,与冠心病基本危险因素BMI呈正相关;EATV大小与冠脉狭窄程度有显著相关性,EATV越大,冠脉狭窄程度越重.EATV对冠心病及再发不良心血管事件发生率及生存时间有较大影响.

目的 探讨不同体质量指数(BMI)的冠心病患者心包周围脂肪含量的差异及意义.方法 选择冠心病患者127例,按照患者BMI分为正常体质量组17例、超重组74例、肥胖组36例.应用德国西门子第二代Somatom Definition Flash炫速双源CT半自动后处理Volume软件测量各组心外膜脂肪(EAT)及心外膜旁脂肪(PAT),观察冠状动脉形态不自然的发生情况.结果 肥胖组、超重组的EAT、PAT均高于正常体质量组,且肥胖组的EAT、PAT均高于超重组(P均<0.05或0.01).BMI与EAT、PAT均呈正相关(r分别为0.464、0.404,P均<0.001).肥胖组中男性EAT高于女性(P<0.05)、PAT低于女性(P<0.01).超重组及肥胖组冠状动脉形态不自然的发生率高于正常体质量组(χ2=9.001,P=0.011).结论 随着BMI的增加,冠心病患者EAT与PAT均明显增大,且与冠状动脉形态学改变有一定关系.

背景 恶性肿瘤蕈状创口,其大量的渗出、恶臭、疼痛等给患者造成生理上的痛苦和心理上的冲击.目的 探讨症状干预联合灵性照护对恶性肿瘤蕈状创口的效果.方法 选取2015年12月—2017年12月在河北医科大学第四医院门诊换药室换药的59例恶性肿瘤蕈状创口患者,治愈性治疗和姑息性治疗共同进行,对恶性肿瘤蕈状创口进行症状干预联合灵性照护,干预前、干预后2、4周分别评价患者疼痛程度、渗出程度、气味等级及采用世界卫生组织生存质量测定量表(WHOQOL-BREF)评估生活质量.结果 干预前后数字分级法(NRS)评分、渗出程度、气味等级、生理领域(PHYS)、心理领域(PSYCH)、独立性领域(IND)、社会关系领域(SOCIL)、环境领域(ENVIR)、精神支柱/宗教/个人信仰(DOM6)评分比较,差异有统计学意义(P<0.05);其中干预后2周渗出程度、气味等级较干预前降低,PHYS、PSYCH、IND、ENVIR、DOM6评分较干预前升高,干预后4周NRS评分、渗出程度、气味等级较干预前、干预后2周降低,PHYS、PSYCH、IND、SOCIL、ENVIR、DOM6评分较干预前、干预后2周升高(P<0.05).结论 症状干预联合灵性照护对恶性肿瘤蕈状创口处理效果良好,可以改善患者躯体症状,提高其舒适度和生活质量,并使患者心灵得到慰藉,人性得到升华,值得临床推广.

Objective To observe the expression levels of p27 and PCNA protein in patients with chronic gastric diseases and in patients with gastric cancer,and to explore the correlation between the expressions of p27,PCNA protein and pathogenesis of gastric cancer.Methods A total of 155 biopsy specimens of gastric mucous membrane by gastroscopy were collected including 32 cases of chronic non-atrophic gastritis,25 cases of chronic atrophic gastritis, 30 cases of intestinal metaplasia,32 cases of atypical hyperplasia and 36 cases of gastric carcinoma. The expression levels of p27 and PCNA protein in gastric mucosa were detected by immunohistochemistry (SP) and statistically analyzed. Results The colouring of P27 protein in gastric cancer tissue superficial parts was relatively strong,however,which in deep part was relatively weak. The expression levels of p27 were gradually decreased in the order of chronic non-atrophic gastritis, chronic atrophic gastritis, intestinal memplasia,atypical hyperplasia and gastric carcinoma,however, the expression levels of PCNA were gradually increased with the order,there were significant differences between two groups each other(P<0.05).Moreover the expression levels of P27 were negatively correlated with chronic non-atrophic gastritis, chronic atrophic gastritis,intestinal memplasia, atypical hyperplasia and gastric carcinoma,(r= -0.976,P<0.01),however,the expression levels of PCNA were positively correlated with the disease types mentioned above (r=0.979,P<0.05).In addition the the expression levels of P27 were negatively correlated with those of PCNA among the 5 groups(r= -0.920,P<0.01).Moreover the expressio nlevels of P27 and PCNA protein were not correlated with patient's age and sex,location and size of tumor (P>0.05).Conclusion The expression levels of P27 and PCNA protein in gastric mucosa are correlated with the pathogenesis and development of chronic gastric diseases,gastric precancerous lesion and gastric cancer,which play an important role during the development process of gastric cancer.

Objective To investigate the effects of metformin on hepatic inflammation, liver fibrosis and insulin resistance in patients with type 2 diabetes mellitus ( T2DM) complicated by nonalcoholic fatty liver disease ( NAFLD). Methods A total of 70 patients with T2DM complicated by NAFLG who were admitted and treated in our hospital from June 2011 to May 2016 were divided into observation group ( n =35 ) and control group ( n =35 ) according to random number table. The patients in control group were treated by diet and exercise only,however,the patients in observation group,on the basis of control group,were treated by oral metformin. After 12-week treatment, the levels of tumor necrosis factor-α( TNF-α), interleukin 6 (IL-6), high sensitivity C reactive protein (hs-CRP), triglyceride (TG), total cholesterol (TC), high density lipoprotein ( HDL-C ) , low density lipoprotein ( LDL-C ) , alanine transferase ( ALT ) , aspartate aminotransferase (AST), type Ⅲ procollagen (PCⅢ), type Ⅳ collagen (Ⅳ-C), hyaluronic acid (HA), laminin (LN), fasting plasma glucose (FPG), 2h postprandial blood sugar glucose (2 hPG), glycosylated hemoglobin (HbA1c), fasting insulin (Fins), insulin resistance index ( HOMA-IR ) and incidence of adverse reactions before and after treatment were observed and compared between the two groups. Results After treatment, the indexes of serum inflammatory factors ( TNF-α, IL-6, hs-CRP) , indexes of blood lipid and liver function ( TC, TG, LDL-C, ALT, AST) , indexes of liver fibrosis ( PCⅢ,Ⅳ-C, LN, HA) and indexes of blood glucose and insulin (FPG, 2 hPG, Fins, HbA1c and HOMA-IR) were significantly decreased in both groups, as compared with those before treatment (P<0. 05). Moreover these indexes mentioned above in observation group were significantly lower than those in control group (P<0. 05), however the levels of HDL C in observation group were significantly higher than those in control group (P<0. 05). In addition there was no significant difference in incidence rate of adverse reactions during treatment between two groups (P>0. 05). Conclusion Metformin can significantly improve hepatic inflammatory reactions of patients with T2DM complicated by NAFLD, relieve liver fibrosis, reduce insulin resistance of body, which is conducive to control blood glucose smoothly, without increasing adverse reactions of patients.

Objective To study the expression of p27 protein and apoptosis index in gastric mucosa of patients with chronic gastric diseases and patients with gastric cancer, and to explore the relationship between the expression of p27 protein in gastric cancer and the evolution of AI. Methods A total of 155 mucosal biopsy specimens including 32 cases of chronic nonatrophic gastritis, 25 cases of chronic atrophic gastritis, 30 cases of intestinal metaplasia, 32 cases of atypical hyperplasia and 36 cases of gastric cancer from outpatients were enrolled in the study. The expression levels of p27 protein and apoptosis index ( AI) were detected by immunohistochemical SP method. Results Yellow or brown granules were located in the nucleus in gastric cancer tissues, with relatively weak staining in superficial layer and relatively strong staining in deeper layer. The expression levels of P27 protein were gradually decreased with the progression of disease. There were significant differences between the groups each other (P<0. 05). The positive staining of apoptotic cells were located in the nucleus, with condensed cytoplasm against the nuclear membrane or stained evenly, without inflammatory reaction around. With the progression of disease ( chronic nonatrophic gastritis, chronic atrophic gastritis, intestinal memplasia, atypical hyperplasia, gastric cancer) , AI was increased gradually in the first three stages, then decreased gradually in the last stages, and there were no significant differences between the first three groups each other (P > 0. 05),however, there were significant differences between the last three groups each other (P<0. 05). In addition, the expression of p27 protein in gastric mucosa of the patients was negatively related to the type of disease (r=0. 976,P<0. 01). However there was no correlation between the expression levels of AI and the type of disease (r=0. 060,P>0. 05),moreover, the expressions levels of p27 protein and AI were not correlated with patient's age, gender, the location and size of the tumor (P>0. 05). Conclusion The expression levels of P27 protein and cell apoptosis are related with the pathogenesis and development of chronic gastric diseases, gastric precancerous lesions and gastric cancer, which play an important role in the evolution of gastric cancer.

目的 探讨绝经后反复阴道出血的患者再次宫腔镜检查的病理类型和意义.方法 回顾性分析2005年1月至2015年1月因绝经后阴道出血就诊于医院的240例患者的临床资料.结果 初次绝经后阴道出血的患者恶性肿瘤共24例,占出血患者的20.0%;子宫内膜不典型增生患者17例,占出血患者的14.2%;子宫内膜良性病变患者56例,占出血患者的46.7%;无组织取出者23例,占出血的19.2%.绝经后再次阴道出血的患者恶性肿瘤共38例,占出血患者的31.7%;子宫内膜不典型增生患者25例,占出血患者的20.8%;子宫内膜良性病变患者54例,占出血患者45.0%;无组织取出者3例,占出血患者的2.5%.绝经后反复阴道出血患者恶性肿瘤检出率为31.7%明显高于首次绝经后阴道出血患者的20.0%,差异有统计学意义(P<0.05).结论 反复绝经后阴道出血子宫内膜癌的风险增加,宫腔镜检查可以更准确的排除子宫内膜癌.

蕈样霉菌病,又名蕈样肉芽肿,是起源于记忆性辅助T细胞的低度恶性的皮肤T细胞淋巴瘤,病程呈慢性渐进性,初期为多种形态的红斑和浸润性损害,以后发展成肿瘤,晚期可累及淋巴结和内脏[1].蕈样霉菌病是一种罕见的疾病,年发病率为0.3/10万,病因与细胞遗传、免疫及环境因素有关.临床分为红斑期、斑块期和肿瘤期,肿瘤期患者预后一般都较差,特别是Ⅳ期(TNM分期)患者[2].河北医科大学第四医院血液内科收治1例蕈样霉菌病ⅣA期患者伴右大腿外侧大面积皮损,临床采用CODP方案(环磷酰胺+长春新碱+含脂质体阿霉素+泼尼松龙)化疗,局部加强换药,取得满意疗效,现报道如下.

目的 探讨盆底康复训练联合护理干预治疗盆底功能障碍性疾病的效果,为临床应用提供依据.方法 盆底功能障碍性疾病患者200例,按入院先后顺序随机分为观察组和对照组,每组100例.对照组仅给予常规盆底康复训练,观察组在其基础上进行护理干预,比较2组患者治疗结束后的盆底肌力改善情况及子宫脱垂、尿失禁和性生活质量的治疗康复情况.结果 治疗后观察组盆底肌力评分1级3例,2级7例,3级32例,4级30例,5级28例.盆底肌力评估3级以上90例,占90%;对照组盆底肌力评分1级18例,2级24例,3级25例,4级20例,5级13例.盆底肌力评估3级以上58例,占58%.观察组盆底肌力改善情况明显优于对照组,比较差异有统计学意义(P<0.05).治疗后观察组子宫脱垂、尿失禁和性生活质量的治疗康复情况均优于对照组,比较差异均有统计学意义(P<0.05).观察组的满意率为96%(96/100),对照组的满意率仅为77%(77/100),观察组满意率明显高于对照组(P<0.05).结论 盆底康复训练联合护理干预治疗盆底功能障碍性疾病疗效显著,患者满意率高,具有临床推广应用价值.

目的 探讨综合护理干预在产后早期盆底功能康复中的应用效果.方法 收集收治的产后6~8周产妇240例,按入院先后顺序随机分为观察组和对照组,每组120例.对照组给予常规护理,观察组实施综合性护理干预,主要措施有心理干预、健康教育、生活干预和盆底功能康复训练.比较2组产妇护理后子宫脱垂分级情况、盆底肌力评定情况、尿粪失禁和性生活满意率.结果 观察组子宫脱垂分级情况好于对照组,盆底肌力恢复情况优于对照组,差异均有统计学意义(P<0.05).观察组产后压力性尿失禁和粪失禁发生率均低于对照组,差异有统计学意义(P<0.05).观察组对性生活满意106例,占88.33%,对照组对性生活满意65例,占54.17%;观察组的性生活满意率高于对照组,差异有统计学意义(P<0.05).结论 实施综合护理干预可有效促进产后早期盆底功能康复,改善其生活质量,具有临床推广应用价值.

目的 探讨宫腔镜手术在绝经期子宫内膜癌患者诊断中的应用价值.方法 子宫内膜癌患者124例,随机分为观察组(宫腔镜手术组,n=65)和对照组(分段诊刮组,n=59),分析比较2组病例相关情况、两种诊断方法术前病理诊断符合率以及2组术前联合诊断分期与手术病理分期结果 比较的准确率.结果观察组术前诊断与术后病理结果符合率为96.9%(其余1例为病理类型不符,1例误诊为癌前病变),高于对照组的81.4%(其余2例为病理类型不符,9例误诊为癌前病变),差异有统计学意义(P<0.05).观察组术前联合诊断分期准确率为Ⅰa期89.7%、Ⅰb期90.7%,高于对照组Ⅰa期64.5%、Ⅰb期64.3%,差异有统计学意义(P<0.05);2组Ⅱ、Ⅲ、Ⅳ期者诊断准确率比较差异无统计学意义(P>0.05).结论 与常规分段诊刮方法相比,宫腔镜手术提高了病理诊断符合率和术前联合诊断分期准确率,有助于临床医师进行临床决策;该方法易于掌握,对硬件设备要求低,适合基层医院开展和推广.

目的 分析本院十年绝经期阴道出血病例资料,探讨绝经期出血病例特点、原因及合理化诊疗建议.方法 选取2007年1月至2016年12月妇科门诊及病房收治的以绝经期阴道出血为主要临床症状首诊患者共1 522例,平均年龄(53.5±7.2)岁;平均绝经6.1年,其中共225例(14.8%)诊断为妇科恶性肿瘤.结果 以每两年为时间节点: 2007至2008年共177例(11.6%)、恶性肿瘤12例(5.3%),2009至2010年共187例(12.3%)、恶性肿瘤21例(9.3%),2011至2012年共302例(19.8%)、恶性肿瘤53例(23.5%),2013至2014年共395例(25.9%)、恶性肿瘤64例(28.4%),2015至2016年共461例(30.3%)、恶性肿瘤74例(32.9%).发病率前三位分别为子宫内膜增生(26.5%)、老年性阴道炎(18.9%)、功血/萎缩性子宫内膜(16.1%);子宫内膜癌发病率(12.0%)远高于宫颈癌(2.8%).约半数绝经期阴道出血就诊患者均为绝经5年内,绝经5年内患者患恶性肿瘤构成比为64.0%,10年内为86.7%.绝经后11～15年、16～20年、>20年三条曲线拟合度较高,此三类人群发病特点相似.结论 对于绝经期阴道出血患者,应根据具体情况进行个体化临床决策:从病史和查体出发,以各种化验检查结果为依据,排除恶性肿瘤可能后考虑各种良性疾病,诊断明确后在对症施治的同时做好患者心理安慰,最后给出随诊建议.

目的：评价三种方法处理糖尿病足溃疡的治疗效果。方法将门诊换药室就诊的60例Wangner分级Ⅱ～Ⅲ级糖尿病足溃疡的患者采用随机数字表法分为生物活性敷料组、新型敷料组和传统敷料组。生物活性敷料组采用联合清创，银离子敷料控制感染，藻酸盐敷料和泡沫敷料管理渗液的换药方法，另外加用生物活性敷料（皮肤创面无机诱导活性敷料德莫林）促进肉芽生长；新型敷料组前期处理同生物活性敷料组，只是未加用生物活性敷料；传统敷料组选用碘仿纱条和凡士林纱布换药。结果伤口处理6周时，伤口愈合总有效率生物活性敷料组明显优于传统敷料组（ P ＜0．01），且生物活性敷料组优于新型敷料组（ P ＜0．05）；伤口面积减小率，伤口愈合计分生物活性敷料组明显优于传统敷料组（ P ＜0．01）。溃疡愈合时间较传统敷料组明显缩短（ P ＝0．000），且优于单纯应用新型敷料换药（ P ＝0．002）。结论新型敷料联合生物活性敷料（皮肤创面无机诱导活性敷料德莫林）治疗糖尿病足溃疡总有效率明显高于传统敷料组，溃疡愈合速率较传统敷料组明显加快，取得满意疗效。

目的：观察宫腔粘连术前两种不同宫颈预处理方法的疗效。方法收集在石家庄市第一医院行宫腔镜治疗的宫腔粘连患者72例。根据术前宫颈预处理方法不同随机分为研究组及对照组。研究组术前3 d晨起空腹顿服米非司酮200 mg，术前2 h阴道后穹窿放置卡前列甲酯栓1 mg。对照组术前30 min给予间苯三酚80 mg静脉注射，术中观察宫颈软化的程度、手术时间、出血量情况，术后观察患者的月经改变。结果研究组宫颈软化的有效率83．33％高于对照组50．00％（ P ＜0．05）。研究组手术时间（26．30±6．25）min、出血量（15．02±5．26）ml低于对照组的（35．64±8．16）min、（23．30±8．09） ml（ P ＜0．05）。术后研究组月经较前好转的明显高于对照组（ P ＜0．05）。结论米非司酮联合卡前列甲酯栓应用于宫腔粘连术前宫颈预处理中，可充分软化宫颈，促进宫颈成熟，减少术中出血，缩短手术时间，且术后月经情况较前好转。

Objective To explore the prognostic factors for patients with severe sepsis and septic shock according to the survival periods.Methods Clinical data of 108 cases of severe sepsis and septic shock treated during Jan.2013 and Dec.2014 were retrospectively studied.The independent risk factors of death was analyzed with Cox porportional haz-ard models.Results The overall mortality was 53.7%,and the mortality at days 0-29,30-89,and 90-365 was 27.8%,24.4% and 15.3%,respectively.Factors significantly associated with mortality included APACHE-Ⅱscore (HR=1.105,95%CI:1.066-1.146),Charlson Comorbidities Index (CCI)(HR=1.390,95%CI:1.210-1.596) and procalcitonin changes (△PCT)(HR=0.996,95%CI:0.995-0.997).Results of stratified analysis according to different survival periods indicated that,APACHE-Ⅱscore (HR=1.104,95%CI:1.052-1.158),CCI(HR=1.462, 95%CI:1.204-1.775),△PCT (HR=0.992,95%CI:0.989-0.995),and days of ICU-stay(HR=0.893,95%CI:0.841-0.949)were the prognostic factors for patients who survived for less than 30 days;risk factors associated with mortality included APACHE-Ⅱscore(HR=1.102,95%CI:1.026-1.183)and △PCT(HR=0.996,95%CI:0.994-0.998)for patients who survived for 30 d-89 d;for patients survived for 90 d-365 d,CCI (HR=1.689,95%CI:1.065-2.679)was the only prognostic factor.Conclusions APACHE-Ⅱscore,CCI and △PCT are the independent&nbsp;prognostic factors for patients with severe sepsis and septic shock,while there were slight differences among different survival periods.It is necessary to develop a multicenter large-scale prospective cohort study for further confirmation.

目的：通过对未婚女性患者实施一般人流和无痛人流的对比分析，及医护人员全面的健康教育指导，使患者更详细的了解无痛人流优于一般人流，为未婚女性人工流产的选择提供理论性依据。方法选取2012年8月至2014年4月妇科门诊就诊的未婚女性患者240例，随机分为无痛有流组和一般人流组，每组120例，观察组未婚女性患者采取无痛人流手术，对照组患者采取一般人流手术进行诊治，对这2组患者进行比较分析。结果2组未婚女性人流患者在手术时间和镇痛效果上观察组明显优于对照组，差异有统计学意义（ P ＜0^．05），而在手术出血量及流产效果上差异无统计学意义（ P ＞0．05），2组均无流产失败病例出现，未婚女性患者无痛人流手术后并发症发生率明显比一般人流组并发症发生率低，差异有统计学意义（ P ＜0．05）。结论为未婚女性患者实施无痛人流手术可以使手术时间缩短，患者疼痛感得到明显改善，有效抑制人流手术后各种并发症的发生，提高患者手术满意度，是终止意外怀孕的一种安全有效方法，在临床上值得广泛应用。

目的：探讨知情同意书对预防化疗患儿液体外渗的作用。方法120例化疗患儿随即分为观察组和对照组，每组60例。观察组由主管护士向患儿家长逐条解读知情同意书的内容，家长理解后签字；对照组仅采用口头宣教的方法。记录2组患儿家长对预防液体外渗的认识程度及化疗患儿发生液体外渗情况。结果观察组宣教方式对患儿家长影响情况较对照组改善明显，观察组液体外渗率为32．27％明显低于对照组的10．23％，差异有统计学意义（ P ＜0．05或＜0．01）。不同宣教方式中认识到液体外渗可以预防者的外渗发生率与不认同液体外渗可以预防者的外渗发生率比较，差异有统计学意义（ P ＜0．01）。结论应用知情同意书可以减少液体外渗的发生率。

目的：了解石家庄市第一医院妇科门诊未婚女性人流的次数、原因及避孕知识知晓状况，为降低的未婚女性再次妊娠率提出预防措施。方法对600例要求人工流产的未婚女性进行问卷调查，包括年龄文化水平人流次数首次性生活的年龄避孕方式避孕知识、药具获得途径等情况进行统计分析。结果600例未婚女性人工流产中年龄16～24岁，平均19岁；首次妊娠268例，再次妊娠332例；未采取避孕措施278例，避孕失败322例，未避孕、避孕失败及重复人流占很大比例。结论未婚女性缺乏科学避孕知识，避孕方法了解不足，避孕信息获得渠道不当，重复流产、高危人流的比例越来越高，为降低未婚女性再次人流的发生，关键是重视青春期男女的生殖健康知识及避孕知识的普及，做好未婚女性人工流产后关爱服务，提高未婚女性生殖健康水平。

目的：建立同时高效液相色谱法同时测定凤仙花中槲皮素、山奈酚的含量。方法色谱柱采用十八烷基硅烷键合硅胶为填充剂（150 mm ×4 u．6 mm，5μm）。甲醇0．4％磷酸溶液（50∶50）；检测波长：360 nm。结果槲皮素在0．1000～4．9987μg范围内，回归方程为Y＝5．608×107X＋8．018×104，R2＝0．9999；山奈酚在0．098～9．777μg范围内，Y＝5．7267×107X ＋3．8767×104， R2＝0．9998，回收率槲皮素为102．27％， RSD 为0．66％；山奈酚为104．16％，RSD为0．83％。结论本方法操作简便，测定结果准确，重复性好，可用于凤仙花中槲皮素、山奈酚的含量测定。