야생 꽃송이버섯의 생리활성 물질을 탐색하여 기능성 식품 개발에 이용하기 위해 꽃송이버섯 물 추출물과 유기용매 분획물의 혈전 용해 활성과 트롬빈 저해 활성, 항산화 활성 및 항염증 활성을 확인하였다. 부탄올 분획물과 에틸아세테이트 분획물이 각각 0.70 plasmin unit과 2.03 plasmin unit의 높은 혈전 용해 활성을 나타내고, 클로로포름 분획물이 83.87%의 높은 트롬빈 저해 활성을 나타냈으며, 에틸아세테이트 분획물이 95.94%의 높은 항산화 활성을 나타냈으며, 클로로포름 분획물은 82.62%의 높은 항염증 활성을 나타냈다. 그러므로 본 연구에서 나타난 꽃송이버섯의 혈전 용해, 트롬빈 저해, 항산화 및 항염증 효과들의 우수한 생리활성 결과들로부터 꽃송이버섯은 혈관계 질환 성인병 치료와 예방을 위한 기능성 식품소재로 활용 가치가 매우 큼을 알 수 있었다. Physiological activities of solvent extracts of Sparassis crispa were investigated for fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities. The fibrinolytic activity was the highest in ethyl acetate extract (2.03 plasmin units/mL) followed by butanol extract (0.70 plasmin units/mL). The ethyl acetate extract exhibited the highest anti-oxidative activity as assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate with a value of 95.94%. The chloroform extract showed thrombin inhibitory activity up to 83.87%. The chloroform extract also showed the highest anti-inflammatory effects on the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. These findings suggest that Sparassis crispa may be a useful material for development of drugs and functional foods.

한국산 흑주름버섯속(Melanophyllum)의 종 다양성과 분포상을 조사하는 과정에서 2종이 확인되었고, M. eyrei는 국내 미기록종과 M. haematospermum은 기존 보고된 종이었다. 종 동정은 형태학적 현미경적 특징을 기초로 이루어졌고, 또한 ITS 염기서열 분석을 통해 이루어졌다. 미기록종은 '청흑주름버섯'으로 신칭하고, 기록종인 잔피막흑주름버섯은 종의 특징을 보완하였다. For the study of species diversity and distribution of Melanophyllum in Korea, M. eyrei and M. haematospermum were identified with unrecorded species and reported species, respectively. Their identification were confirmed based on macroand microscopic descriptions as well as ITS region sequence data. We give a Korean name 'Cheongheukjureumbeoseot' for M. eyrei and provided full description for M. haematospermum due to incomplete previous description.

본 연구는 야생버섯으로부터 혈관계질환 관련 생리활성을 확인하여 혈관계질환을 위한 기능성식품 개발에 재료로 사용하기 위해 야생버섯 33종의 야생버섯 메탄올 추출물을 제조하여 심혈관계 질환 생리활성을 확인하였다. 긴대밤그물버섯(Boletellus elatus)과 벽돌빛뿌리버섯(Heterobasidion insulasis)이 높은 혈전용해 활성과 트롬빈저해효과, 항산화활성을 나타내 혈관계질환 치료나 예방을 위한 기능성식품 개발에 이용 가능할 것으로 기대된다. In this study, the biological activities of 33 kinds of wild mushroom methanol extracts were investigated. Boletellus elatus showed the greatest fibrinolytic activity (1.08 plasmin units/mL) in a fibrin plate assay, and the activity of Heterobasidion insulasis was 0.89 plasmin units. The thrombin inhibitory activities of Boletellus elatus and Heterobasidion insulasis were 93.32% and 93.69%, respectively. In a ${\alpha}$-glucosidase inhibitory activity test, Laccaria amethystina showed the greatest inhibitory activity at 81.25%. The antioxidative activities of Gomphus sp. and Geastrum lageniforme were 91.37% and 90.42%, respectively. Since Boletellus elatus and Heterobasidion insulasis have strong fibrinolytic and thrombin inhibitory and antioxidative activities, the two mushrooms can be used as material for the development of biofunctional foods for cardiovascular diseases.

본 연구는 한국산 주름버섯목의 종 다양성과 분포상을 조사하는 과정에서 경기도 용문산에서 한국미기록 긴대개암버섯 "Hypholoma elongatum(Pers.) Ricken"을 채집하여 형태적 특성에 준하여 분류동정 하였다. 자실체의 형태적 특징에 대한 상세한 기술과 미세구조를 도해하고 한국명을 신칭하여 보고하고자 한다. 본 연구에 사용된 표본은 국립농업과 학원 식물균류표본보존센타(HCCN)에 보존되어 있다. Different mushroom species have collected from various areas and their taxonomic identification was carried out for species diversity. Among them, Hypholoma elongatum (Pers.) Ricken that was collected from Yongmoon mountain of Gyonggi in Korea was confirmed as new collection in Korea and registered here with descriptions and Korean common name. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

One rare and interesting species collected from Seorak-san, Inje-gun; Yeonyeop-san, Hongcheon-gun; Daeam-san, Yanggu-gun, Gangwon-do; Pocheon-gun, Gyeonggi-do; Songni-san, Boeun-gun; Joryeong-san, Goesan-gun, Chungcheongbuk-do and Sobaeksan, Yeongju-si, Gyeongsangbuk-do is described and illustrated in detail. The species "Protodaedalea hispida Imazeki" and genus "Protodaedalea Iamzeki" has not been previously recorded in Korean fungal flora. The specimens have been deposited in the Herbarium Conservation Center of the National Academy of Agricultural Sciences.

저자들은 한국산 균류의 종다양성 조사를 실시하여 왔으며, 그 중 주름버섯목 의 무명버섯속, 만가닥버섯속, 그늘버섯속, 외대버섯속과 젖버섯속에 속하는 5종 (장미무명버섯, 애만가닥버섯, 흰그늘버섯, 꼬마외대버섯과 치마젖버섯)이 한국미 기록종으로 확인되었다. 미기록종에 대한 미세구조와 특징을 기술하고 한국명을 신칭하여 보고한다. 사용된 모든 표본은 국립농업과학원 식물균류표본보존센타 (HCCN)에 보존되어 있다. The investigation on the species diversity for the Korean fungal flora was carried out by authors. Five species of the Agaricales recorded in the present paper are distributed Hygrocybe laeta (Pers.)P. Kummer var. laeta, Lyophyllum tylicolor (Fr.) M. Lange & Sivertsen, Clitopilus scyphoides (Fr.) Singer, var. scyphoides, Entoloma rusticoides (Gillet) Noordel. and Lactarius uyedae Singer were recorded for the first time and are described and illustrated in detail. Specimens cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

One rare and interesting species collected from Gyeryong-san, Chungnam Province is described and illustrated in detail. The species "Tectella patellaris (Fr.) Murr." and genus "Tectella Earle" is a first record for Korean fungal flora. Specimens cited here have been deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

One interesting parasitic forming fungus, being grown on the eggs of Phallus impudicus was collected from Mulchajoreum and on woods of Saryeoni in Jeju island. This taxon identified as a solmsii (E. Fisch.) S. Imai f. octospora Yoshim. (Hyporeaceae) based on morphological characteristics. According to the interpretation of Doi(1978), this taxon hsving the stipitate, genus Podostroma, family Hypocreaceae, Hypocreales, Ascomycota was identified as solmsi f. octospora and its korean common name designated by authors. This species is characterized in having the fusiforme to ellipsoidal, unicellular and warted ascospores without septate and with blunt apiculus at each end and mycoparasitic habitat. Although there is some mycologists suggest that this taxon having above characters needs to be derived from Hypomyces. But authors are primarily going to record this species as a named Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi for the fungus flora of Korea. In a future, it is need in detail to compare their DNA sequencing data on the type species and some related species. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

This study contains descriptions and illustrations of three new and noteworthy taxa of the genus Psathyrella from Korea. Among them, Psathyrella pervelatoid, P. koreana, and P. utriformcystis are new to science. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

Sejong Institute of Data Analysis, Suwon 443-766, Korea(Received December 10, 2010. Accepted December 23, 2010)ABSTRACT: Dead insects infected with fungi were collected from mountains and islands in several locations inKorea from 2003 to 2007. Morphological characteristics of the fungal fruiting bodies produced on the insect sampleswere examined. A total of 542 entomopathogenic fungal isolates were obtained from the insect samples and iden-tified based on their morphological and cultural characteristics. Three species of Beauveria, eight species ofCordyceps and four species of Isaria were mainly isolated from the insect samples. The other entomopathogenic fungiisolated from the insect samples were Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp. and Verticillium sp.KEYWORDS : Beauveria, Cordyceps, Entomopathogenic fungi, Isaria

Clitocybe alboinfundibulliforme sp. nov. is widely distributed in Korea. Volvariella koreana sp. nov. is rarely distributed in Korea. These taxa were occasionally found together at the same place. Both of these species seem to be associated with each other. These two species are fully described and illustrated in this paper.

Isolation of Inonotus obliquus from Betula costata in KoreaTaxonomic Study on Some Unrecorded Taxa of Korean AphyllophoralesSurveys of Host Plants of Pestalotiopsis spp. in KoreaBacillus subtilis B...

Specimens belonging to the genera Mycena and Collybia which have been preserved in the herbarium of National Institute of Agricultural Sciences and Technology, Suwon, Korea were investigated. Among them, four species were confirmed to be Hydropus species viz Hydropus marginellus, H. nitrata, H. erinensis, and H. floccipes. Authors registered them to the Korean mycoflora with full descriptions, illustrations and Korean names.

s of the 2005 Fall Meeting of the Korean Society of Mycology 257 groups. The first group included one species, C. militaris. The second group included four species, C. yongmoonensis, C. pruinosa, Shimozuomyces poradoxa. The third group was one species, C. scarabaeicola.With the comparison of sixteen isolates of C. militaris, we found that there was not exists intraspecific variation in the gpd gene of C. militaris. Notes on Two Species of the Laboulbeniales from Tibet Yong-Bo Lee 1 *, Chae-Kyu Lim 2 , Dong-Kyoung Jang 3 , In-Ha Jung 3 , Sang-Hee Park 3 , In-Hwa Jang 3 , Sung-Eun Yun 3 and Hansu Park 4 1 Division of Science Education, College of Education, Chosun University. 2 Dept. of Herbal Medicine Resources development, Naju College. 3 Major in Biology Education, Graduate School of Education, Chosun University. 4 Major in Biology Education, Graduated School, Chosun University Two species of the Laboubeniales based on the preparations of Tibet are described. They are new to the mycological flora of Tibet. Laboulbenia polypaga were found on several parts of Amara majuscula, this species have the outer appendages not ramified, simple. Peyritschiella protea were found on the lower abdomen of Philonthus wuesthoffi, this species have two perithecia and two antheridia produced on the third layer of receptacle. Phylogenetic Analysis of Nuclear Ribosomal DNA Intergenic Spacer (IGS) I Region of Genus Agaricus Young Hyun Ryu 1 *, Woo Sik Jo 1 *, Sung Guk Choi 1 *, Jong Guk Kim 2 , Jae Tak Yoon 3 and Jung Sik Park 4 1 Dept. Plant Environment, GyungBuk ATA. 2 Dept. Microbiology, Kyungpook Natl. Univ. 3 GyungBuk ATA. 4 NIAST, RDA Phylogenetic relationship of genus Agaricus was studied by comparing the nuclear ribosomal intergenic spacer (IGS) I region and structural characteristics was analyzed. IGS I region of 5 species (A. bisporus, A. edulis, A. bitorquis, A. campestris, A. potobella) of Agaricus was from 1,323 bp (A. bisporus ASI 1056) to 1,491 bp (A. potobella ASI 1210) long and GC contents were 47% (A. bisporus) to 49% (A. potobella). Size difference of IGS 1 region among Agaricus species was caused by sequence insertion in 3' end region, especially A. potobella. and inserted sequence was higher GC content than other IGS sequence and have repeated GG/GC patterns. ITS region was widely used in phylogenetic relation of basidiomycetes, but fungal ribosomal IGS region was not well understood and applied yet. Our study indicated that IGS region can be a good tool in phylogenetic study of basidiomycetes. The Identification and Characterization of Halophilic Fungi, Eurotium spp., Isolated from Marine Anchovies Su Young Kim 1 , Hee-Gon Choi 1,2 , Mira Jin 1 and Kwang-Yeop Jahng 1,3 * 1 Division of Biological Sciences, Chonbuk National University. 2 Research Institute of Water Environment, CNU. 3 Basic Science Research Institute, CNU We have isolated halophilic fungi from Korean traditional food, jeotkal, which is madeof salted anchovies. These filamentous fungi that formed the sexual structure cleistothecium could not grow without salt in growth medium. Spores were lysed by osmostress under no salt condition so that they could not germinate. All strains from anchovy jeotkal were also isolated from the surface tissue such as scales and gills of raw anchovy, but not from liver and digestive organs, implying that these isolates originated from sea water. The phylogenetic analyses based on the sequence comparison of 18S, 28S and ITS region of rDNA suggested that they classified into the genus Eurotium. Species of Eurotium typically produce bright yellow cleistothecia and dull green spinose conidia which are formed from phialides only. The nucleotide sequence of 28S region of isolates showed 100~99% identity to that of E. herbariorum and E. rubrum, suggesting that they should be highly related to these species. Morphological, physiological and molecular characteristics further supported that these fungal isolates belong to halophilic species of the genus Eurotium. Fungal Volatile from a Strain of PGPF, Cladospoprium sp. CL1 can Enhance Plant Growth and Induce Systemic Resis-

Abstract For investigation of the species diversity of the Genus Crepidotus in Korea and constructing a key to Korean Crepidotus species, a total of 65 specimens, collected from 18 locations from 1982 to 2002, were observed for morphological characters of carpophores and other features. All the specimens have been preserved in the herbarium of the National Institute of Agricultural Sciences and Technology, R.D.A., Suwon, Korea. The specimens were identified according to the classification systems given by Hesler and Smith (1965), Nordstein (1990), Orton (1960), Pilat (1948), Senn-Irlet (1991, 1992, 1993) and Singer (1951, 1973, 1986). In this study, a total of 10 Crepidotus species were confirmed. Among them, Korean common names were designated to six unrecorded species as follows: C. uber, “적귀버섯”; C. hygrophanus, “곤약귀버섯” C. latifolius, “꼬마무리귀버섯”; C. obscurus, “먼지귀버섯”; C. subverrucisporus, “분롬귀버섯”; and C. circinatus, “노년고리귀버섯”. A key to 10 Crepidotus species has been constructed.