Abstract South American ecosystems harbour diverse cricetid rodents and their associated nematode parasites, with implications for ecology and zoonotic risk. We conducted a systematic review following the PRISMA guidelines to catalogue nematode taxa reported from the digestive tract and accessory organs of cricetid rodents in Argentina and Chile. Searches were carried out in five databases (PubMed, WoS, Scopus, SciELO, and Google Scholar) up to 10 January 2026. Parasitological descriptors, such as prevalence (P), mean abundance ( M A), and mean intensity ( M I) were estimated. We analysed 53 studies examining 4400 rodents (33 species), with Abrothrix olivacea ( n = 1000 specimens) and Akodon azarae ( n = 775 specimens) most frequently sampled. Ninety-two nematode taxa were reported, with A. olivacea exhibiting the highest richness (17 taxa ). Guerrerostrongylus zeta showed the highest P (97.56% in Oligoryzomys nigripes ), followed by Stilestrongylus stilesi (92.5% in Necromys lasiurus ) Peak infection intensities occurred with Stilestrongylus flavescens in O. flavescens ( M A = 72.79/ M I = 126.7) and St. stilesi in N. lasiurus ( M A = 120/ M I = 129.73). This review provides the first comprehensive synthesis of gastrointestinal and hepatopancreatic nematodes in cricetid rodents from Argentina and Chile, highlighting research biases and priorities for advancing our understanding of nematode–cricetid rodent dynamics in the region. The overrepresentation of A. olivacea underscores the need to expand the range of cricetid rodent sampled and to increase the use of non-invasive and molecular diagnostic tools. Addressing these gaps will improve our understanding of nematode diversity and associated zoonotic risks in cricetid rodents in these countries.

Myasis caused by Cochliomyia hominivorax larvae (Coquerel, 1858), represents a significant sanitary and economic challenge under the One Health approach, affecting human health, livestock production, and biodiversity. Studies on the spatial ecology and distribution of this species in South America (SA) have been scarce. This study aimed to model real and potential suitability areas for C. hominivorax in SA, based on myiasis cases in humans and other vertebrates reported in the scientific literature. The MaxEnt model was developed using WorldClim bioclimatic variables clipped to SA, with 1,000 iterations performed to generate mean logistic distribution models. To mitigate collinearity variables with an absolute correlation coefficient of ∣r∣ < 0.7 were retained. Jackknife tests determined the relative importance of the bioclimatic variables. Brazil and Argentina contained the majority of records, followed by Colombia and Venezuela. Paraguay and French Guiana contributed fewer occurrences. The most frequently recorded hosts were cattle, cats, dogs, and pigs, with great numbers of human cases documented in Brazil and Argentina. Predictive models indicated that west-central Amazonia, eastern Paraguay, northern Argentina, northeastern Brazil, and several countries in northern SA represent moderate to high suitability for species presence (habitat suitability scores 0.80 to 1.00; AUC 0.885). The potential distribution of C. hominivorax in SA was determined by humidity and temperature gradients in tropical and subtropical environments, excluding arid or cold zones. These findings emphasize the need to strengthen epidemiological surveillance and transboundary control strategies, with special attention to domestic hosts, wildlife movement, and human cases in the Southern Hemisphere.

Chagas disease (CD) is a zoonosis caused by the hemoflagellate Trypanosoma cruzi, which is transmitted by insects of the subfamily Triatominae. Studies to identify the vectors involved in the transmission of T. cruzi have been conducted in the Caribbean regions of Colombia, Venezuela, and Panama. However, retrospective studies and comprehensive reviews covering these three countries are still lacking, limiting our understanding of this issue. This study used the PRISMA method to conduct a systematic review of studies on the triatomine fauna and its natural infection by T. cruzi in the Caribbean regions of Panama, Colombia, and Venezuela, using descriptors such as “Chagas disease,” “Triatominae,” and “Trypanosoma cruzi” applied individually for each state, department, or province of the three countries. A total of 3477 studies were identified through a search of the PubMed, Scielo, and ScienceDirect databases. After the selection process, 48 articles were included, with six additional articles obtained through indirect search methods. A total of 18,344 triatomines belonging to 14 species of seven genera were identified from these 48 articles. The most common species was Rhodnius pallescens (Barber) with 7540 individuals (41.10

Sucking lice (Anoplura) are highly specific parasites that depend extensively on their mammal hosts for survival. However, the climate to which their hosts are exposed can cause changes in their populations, independent of the protection provided by their hosts. Despite its harsh climate, arid northern Chile is home to a wide variety of rodents, particularly cricetids. This area comprises four distinct ecoregions with different sub‐climates. We aimed to evaluate the spatial and seasonal variability of sucking lice (Anoplura) parasitizing rodents in northern Chile. Specifically, we analysed the association of environmental variables and host density with the louse point prevalence (P%), mean abundance ( M A) and mean infestation intensity ( M I). Rodent samples were collected during autumn and spring of 2011 and summer and winter of 2012 in 13 sites representative of the Coastal Desert (CD), Continental (Interior) Desert (ID), Andean Tropical (AT) and Pre‐Andean Tropical (PAT) ecoregions. A total of 992 rodents of eight species were captured and anaesthetized, and lice were collected and identified. The P%, M A and M I of lice were estimated. The association of climatic variables (temperature, precipitation and relative humidity) and host density (capture success as a proxy) with louse P% and M A was assessed using Spearman correlation and Fisher's test. Four lice species were identified based on external morphology (Hoplopleuridae: Hoplopleura aitkeni Johnson, Hoplopleura reducta Ferris, Hoplopleura sp. travassosi group; Poliplacidae: Polyplax spinulosa (Burmeister)). Hoplopleura aitkeni and H. reducta showed significant seasonal differences in P%, M A and M I in the AT and PAT ecoregions. In contrast, the H. sp. travassosi group showed no significant seasonal variation. Polyplax spinulosa was found exclusively on introduced rodents in the ID ecoregion, with distinct seasonal variations observed in Rattus norvegicus Berkenhout and Rattus rattus Linnaeus (Muridae). We reported 17 new host–parasite associations, and one lice species not previously described in the country. This study demonstrates that seasonal environmental variation does not equally impact all lice species, emphasizing the need for further research into the ecological and biological drivers of these dynamics.

Introduction: Dengue is an arboviral disease transmitted by the Aedes aegypti mosquito, which is common in urban areas of the Colombian Caribbean region. Effective control depends on social, environmental, and educational factors. Objective: To examine the social representations (SR) associated with dengue among students from an educational institution in the Colombian Caribbean region. Method: A descriptive study was conducted with seventh-grade students from an educational institution in Barranquilla. Semi-structured surveys containing 17 questions (14 closed/3 open-ended) assessed students' SR regarding dengue. Results: Sixty students participated in the study; 83.33% of students reported never having had dengue, but all identified A. aegypti as the vector. Some misconceptions about transmission and prevention persisted. The most frequently reported conditions were uncontrolled water storage (30%) and stagnant water near homes (36.67%). Conclusions: Students' knowledge of dengue was fragmented, highlighting the need for ongoing educational efforts to improve understanding in this population.

Chagas disease is the main endemic vector-borne anthropozoonosis in the Americas, a potentially fatal disease caused by the parasite Trypanosoma cruzi. The objective of this study was to retrospectively determine the seroprevalence of Chagas disease in an endemic community in Anzo & aacute;tegui State, Venezuela. An observational, descriptive, cross-sectional epidemiological study was conducted with 161 blood samples from individuals taken in 2018 and collected on filter paper. Enzyme-linked immunosorbent assay (ELISA) was used for processing, and for positive results, polymerase chain reaction (PCR) was applied. Fifteen positive results (9.32%) were obtained using the ELISA method, of which 14 (8.70%) were positive using the PCR technique. A seroprevalence of 9.32% for Chagas disease was observed. The sociodemographic characteristics reported that the age groups with greater frequency corresponded to 0-10 years (25.46%) and over 50 years (23.6%), female sex (50.93%), social stratum IV (57.14%), primary school and high school education level (46.58%). Likewise, a higher frequency of students (34.16%), ranch-type housing (59.63%), with ignorance of the vector (73.29%), ignorance of the disease (68.32%) and asymptomatics (93.17%). Risks (OR): ranch-type housing (OR: 1.97; p = 0.38), farmer (OR: 3.42; p = 0.50), heart disease (OR: 2.34; p = 0.27), vector bite (OR: 1.17; p = 0.93). The risk of exposure to Trypanosoma cruzi has continued to persist in recent years, with a higher frequency among individuals with agricultural occupations and poor housing conditions.

Las parasitosis afectan a millones de personas en todo el mundo, mayormente en países en desarrollo. Los niños son el grupo más vulnerable por su sistema inmunológico en desarrollo, hábitos higiénicos poco consolidados y mayor exposición al ambiente. Esta revisión sistemática identificó parasitosis reportadas en niños de Paraguay entre 2014 y 2024. Se empleó una metodología documental y estudio retrospectivo, siguiendo las guías PRISMA-SR y JBI. Se incluyeron estudios experimentales y observacionales, excluyendo revisiones narrativas. Se emplearon las bases de datos MEDLINE, Embase, Scopus y literatura gris. Dos revisores seleccionaron y extrajeron los datos utilizando Rayyan QCRI y Excel, la calidad del proceso se evaluó con la JBI Critical Appraisal Tool. Se identificaron 537 artículos, de los cuales 21 cumplieron los criterios de inclusión. Estos trabajos reportaron infecciones parasitarias más frecuentes ocasionadas por Giardia lamblia, Blastocystis hominis y Ascaris lumbricoides (76%). El 95% de las muestras fueron diagnosticadas por el método coproparasitológico. La mayoría de las muestras procedían de Concepción, Central y Alto Paraná. Los principales factores de riesgo fueron edad, hacinamiento, poco acceso a agua potable y saneamiento deficiente. Las parasitosis fueron más prevalentes en niños indígenas y en viviendas con piso de tierra. Se reveló la existencia de alta prevalencia de parásitos intestinales en comunidades vulnerables. El registro de hemoparásitos fue escaso, sugiriendo el subregistro de estas parasitosis. Es esencial mejorar el diagnóstico mediante técnicas moleculares e inmunológicas y promover estrategias de prevención para reducir la carga de parasitosis en Paraguay.

Motivation for the study. The role of bats as hosts of Trypanosoma spp. in the Atlantic department in Colombia, as well as its taxonomic diversity has been poorly studied. Main findings. This is the first report of frequency of infection by Trypanosoma spp. in bats in the Atlántico Department in Colombia. Implications. The great adaptive capacity of bats to different ecological niches and its role as hosts of Trypanosoma spp. for wild and urban ecotopes represents a risk factor in transmission cycles of epidemiological importance. We conducted a study to evaluate the frequency of infection by Trypanosoma spp. in bats captured in wild and urban ecotopes in the Department of Atlántico in the Caribbean region of Colombia from March 2021 to May 2022. Bats were taxonomically identified, and sex, relative age, and reproductive conditions were determined. A blood sample was used for parasitological analysis and DNA extraction to amplify a region of the 18S rRNA. 125 bats were collected, with the most abundant families being Molossidae (62/125; 49.6%) and Phyllostomidae (43/125; 34.4%). Molossus molossus collected in wild habitats showed an infection frequency of 8.1% (5/61) and 4.1% (3/61) through parasitological and molecular analysis, respectively. In comparison, Noctilio albiventris collected in urban habitats showed an infection frequency of 16.6% (2/12) for both analyses. These findings represent the first records of M. molossus harboring trypanosomes for the Department of Atlántico and of N. albiventris harboring trypanosomes in Colombia.

Phlebotomine sand flies are critical vectors of Leishmania parasites, impacting public health significantly. This study focused on assessing the diversity of sand flies in a rural area of El Carmen de Bolívar Municipality, northern Colombia, employing rarefaction curves and Hill numbers to understand potential vector communities and inform environmental management. From January 2018 to April 2019 (five samplings), sand flies were collected using CDC light traps with blue LED in domestic/peridomestic/sylvatic ecotopes, identifying species per Young and Duncan (1994) and Galati (2003). Hill numbers provided diversity estimates across samples, while Principal Component Analysis correlated with environmental factors with phlebotomine species presence and abundance. 8,784 phlebotomine individuals were collected; 56,4% females and 43.6 % males (ratio 3:2). These individuals belonged to eight species: Pintomyia evansi, Psychodopygus panamensis, Lutzomyia gomezi, Micropygomyia cayennensis, Evandromyia dubitans, Psathyromyia aclydifera, Pintomyia serrana, and Pintomyia rangeliana; with Pi. evansi being the most abundant species (74.39%; 6,530 exemplars). The ANOVA showed no significant differences between phlebotomine sand flies abundances across ecotopes (p=0.018). Species of epidemiological relevance as Pi. evansi and Lu. gomezi not show a positive correlation with environmental variables evaluated, only Ps. panamensis was positively correlated with precipitation. However, the study emphasizes the need for a continuous sand fly monitoring and research to enhance leishmaniasis control strategies, highlighting the necessity to expand knowledge on phlebotomine diversity and environmental interactions to understand vector ecology and disease dynamics better.

Introduction. El Alf & eacute;rez, a village in Los Montes de Maria (Bolivar, Colombia) and a macro -focus of leishmaniasis, recorded its first case in 2018, evidencing changes in the distribution and eco-epidemiology of the disease, although interactions between vectors and local fauna remain unknown. Objective. To evaluate the diversity of sandflies and their blood meal sources in the community of El Alferez in the municipality of El Carmen de Bolivar (Bolivar, Colombia). Materials and methods. In 2018 , sandflies were collected using LED -based light traps in domestic, peridomestic, and sylvatic ecotopes and identified at the species level. Multiplex polymerase chain reaction targeting the mitochondrial cytochrome B gene was used to analyze blood from the digestive tract. Results. Lutzomyia evansi was the most abundant species (71.85%; n = 485/675), followed by Lu. panamensis , Lu. gomezi , Lu. trinidadensis , Lu. dubitans , Lu. abonnenci , and Lu.aclydifera. Twenty-five percent of the species had blood meals from Canis familiaris (36.00%; n = 9/25), Ovis aries (36.00%; n=9:/25), Bos taurus (24.00%; n = 6/25) , Sus scrofa (20.00%; n = 5/25), and Homo sapiens (8.00%; n = 2/25). Lutzomyia evansi registered the highest feeding frequency (68.00%; n = 17/25), predominantly on a single (44.00%; n = 11/25) or multiple species (24.00%; n = 6/25). Conclusion. Results indicate a eclectic feeding behavior in Lu. evansi , implying potential reservoir hosts for Leishmania spp. and increasing transmission risk. This study is a first step towards understanding the diversity of mammalian blood sources used by sandflies, that may be crucial for vector identification and formulation of effective control measures.

Introducción. En 2018, en la vereda El Alférez de Los Montes de María (Bolívar, Colombia), un macrofoco de leishmaniasis, se reportó el primer caso y se evidenciaron cambios en la distribución y ecoepidemiología de la enfermedad. No obstante, las interacciones entre vectores y fauna local aún son desconocidas.Objetivo. Evaluar la diversidad de flebotomíneos y sus fuentes de alimentación sanguínea en la comunidad de El Alférez del municipio de El Carmen de Bolívar (Bolívar, Colombia).Materiales y métodos. En el 2018, se recolectaron flebotomíneos mediante trampas de luz led ubicadas en el domicilio, el peridomicilio y en el área silvestre, y se identificaron a nivel de especie. Se utilizó la reacción en cadena de la polimerasa múltiple dirigida al gen mitocondrial citocromo B para analizar la sangre del aparato digestivo.Resultados. Lutzomyia evansi fue la especie más abundante (71,85 %; n = 485/675), seguida por Lu. panamensis, Lu. gomezi, Lu. trinidadensis, Lu. dubitans, Lu. abonnenci y Lu. aclydifera. El 25 % (n = 25/100) de las especies analizadas tuvieron como fuentes de ingesta sanguínea a Canis familiaris (36 %; n = 9/25), Ovis aries (36 %; n = 9/25), Bos taurus (24 %; n = 6/25), Sus scrofa (20 %; n = 5/25) y Homo sapiens (8 %; n = 2/25). Lutzomyia evansi fue la especie con la mayor frecuencia de alimentación (68 %; n = 17/25), predominantemente de una sola especie (44 %; n = 11/25) o de varias (24 %; n = 6/25).

Introducción: Aedes aegypti es un insecto del grupo de los culícidos, preferencialmente antropofílico, que actúa como vector de algunos arbovirus, como del dengue, Zika y chikungunya y fiebre amarilla, con una amplia distribución a nivel de trópicos y subtrópicos y responsables de brotes epidémicos importantes en el Paraguay. La urbanización, la globalización y la falta de un control eficaz, favorecen su permanencia. Estudiar la diversidad genética de esta especie permitirá evaluar la dinámica de sus poblaciones y su adaptación a los cambios ambientales, para guiar su control. Objetivo: Estimar la diversidad genética de las poblaciones de Ae. aegypti de los departamentos Central y Cordillera del Paraguay utilizando marcadores de inter- repeticiones de secuencia simple (ISSR). Metodología: Se seleccionaron 40 mosquitos hembra, 20 del departamento Central y 20 del departamento de Cordillera correspondientes a la región del Chaco Húmedo, Paraguay, para la amplificación de los marcadores genéticos ISSR mediante PCR, utilizando los cebadores Rhea-4, ISSR-7, ISSR1 y Ae-ISSR-6. El grado de segregación de las poblaciones estudiadas con un AMOVA y la diferenciación entre ellas con el índice de fijación FST, fueron determinadas mediante el programa Arlequín versión 3.5.2.2. Resultados: Se identificaron 36 loci, 90,91 % de ellos polimórficos. La mayor parte de la diferenciación genética se debió a variaciones dentro de las poblaciones y el índice de fijación entre poblaciones (FST = 0.0432). Discusión: Es posible que la diversidad dentro de las poblaciones de Central y Cordillera se deba a las características heterogéneas en cuanto a urbanismo y semi-ruralidad y la existencia de ecotonos, donde circulan estas poblaciones. Los resultados revelaron una diferenciación genética interpoblacional moderada, indicativo de que no están completamente aisladas genéticamente.

Trypanosoma cruzi and Leishmania spp. coexist in several endemic areas, and there are few studies of Chagas disease and leishmaniasis coinfection worldwide; for this reason, the objective of this work was to determine the Chagas disease and leishmaniasis coinfection in several rural communities co-endemic for these diseases. A total of 1107 human samples from six co-endemic rural communities of Cojedes state, Venezuela, were analyzed. Serum samples were evaluated by ELISA, indirect hemagglutination, and indirect immunofluorescence for Chagas disease diagnosis, and individuals were evaluated for leishmaniasis by leishmanin skin test (LST). Approximately, 30

It is estimated that 6–7 million people worldwide are infected with Trypanosoma cruzi , the parasite that causes Chagas disease. In Venezuela, Chagas disease remains a public health problem. In this work, T. cruzi isolates from six species of triatomines and mammals of the orders Didelphimorphia and Xenarthra, captured in rural communities of Monagas, underwent parasitological and molecular characterization. A total of 471 triatomines and 17 mammals were captured, with a natural infection rate of 41.4% and 70.6%, respectively. In the male NMRI mouse model used for parasitological characterization (Prepatent period, parasitemia curve, mouse mortality and tissular parasitism), T. cruzi isolates exhibited high lethality due to their pronounced virulence, irrespective of the parasite load in each mouse, resulting in a mortality rate of 75%. Among the vector isolates, in the mouse model only 2 out of 6 remained alive, while the rest perished during the evaluation. Conversely, the isolates from mammals proved fatal for all the inoculated mice. All isolates were identified as belonging to DTU TcI, based on the molecular markers as intergenic region of the miniexon, D7 divergent domain of the 24Sα rDNA, size-variable domain of the 18S rDNA and hsp60-PCR-RFLP- Eco RV. This study demonstrates the presence of vectors and mammalian reservoirs naturally infected with T. cruzi in communities of Monagas, the 9th largest and 9th most populous state in Venezuela. This situation represents a neglected epidemiological problem demanding urgent attention and imperative health care intervention.

We studied the prevalence of infection by trypanosomatid parasites in Didelphis marsupialis and its relationship with morphological/age aspects in a rural area of El Carmen de Bolivar, Colombia. Five visits were made to the Vereda El Alférez; each of which lasted three consecutive nights. During these visits, Tomahawk® traps were installed in the peridomestic and wild ecotopes of the Vereda El Alférez. Body measurements, sex and age were determined from the collected animals. Blood was extracted by cardiopuncture, after sedation, in order to obtain total deoxyribonucleic acid (DNA) and amplify the conserved region of the kinetoplast minicircle DNA (kDNA) of parasitic trypanosomatids. The association between morphological parameters of didelphids and their frequency of infection by parasitic trypanosomatids was determined by binomial regression. Thirty D. marsupialis specimens (60.0% females and 40.0% males/66.7% adults and 33.3% juveniles) were collected. Molecular diagnosis revealed a frequency of trypanosomatid parasite infection of 46.7%. Stage (p=0.024) was a determinant for infection. We discuss the role of D. marsupialis as a potential reservoir of parasitic trypanosomatids in the Vereda El Alférez.

Se evaluó la prevalencia de infección por parásitos tripanosomátidos en Didelphis marsupialis y su relación con los aspectos morfológicos/etarios en una zona rural de El Carmen de Bolívar, Colombia. En cinco visitas (2018-2019) se instalaron trampas Tomahawk® en los ecótopos peridoméstico y silvestre en la Vereda El Alférez, durante tres noches consecutivas/visita. A los animales recolectados, se les determinaron medidas corporales, sexo y edad; y se les extrajo sangre por cardiopuntura, previa sedación, para extracción del ácido desoxirribonucleico (ADN) total y amplificación de la región conservada del ADN de minicírculos de kinetoplasto (ADNk) de parásitos tripanosomátidos. La asociación entre parámetros morfológicos de los didélfidos y su frecuencia de infección por parásitos tripanosomátidos fue determinada mediante una regresión binomial. Se recolectaron 30 individuos de D. marsupialis (60,0% hembras y 40,0% machos/66,7% adultos y 33,3% juveniles). El diagnóstico molecular reveló una frecuencia de infección por parásitos tripanosomátidos del 46,7%. El estadio (p=0,024) fue determinante para la infección. Se discute el papel de D. marsupialis como potencial reservorio de parásitos tripanosomátidos en la zona evaluada.

Panstrongylus is a Neotropical taxa of 16 species, some more widespread than others, that act as vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease (CD). This group is associated with mammalian reservoir niches. There are few studies of the biogeography and niche suitability of these triatomines. Using zoo-epidemiological occurrence databases, the distribution of Panstrongylus was determined based on bioclimatic modelling (DIVA GIS), parsimonious niche distribution (MAXENT), and parsimony analysis of endemic species (PAE). Through 517 records, a wide presence of P. geniculatus, P. rufotuberculatus, P. lignarius, and P. megistus was determined and recorded as frequent vectors of T. cruzi in rainforest habitats of 24–30 °C. These distributions were modeled with AUC >0.80 and <0.90, as well as with the seasonality of temperature, isothermality, and precipitation as relevant bioclimatic variables. Individual traces for each taxon in Panstrongylus—1036 records—showed widely dispersed lines for frequent vectors P. geniculatus, P. lignarius, P. rufotuberculatus, and P. megistus. Other occasional vectors showed more restricted dispersal, such as P. howardi, P. humeralis, P. lenti, P. lutzi, P. tupynambai, P. noireaiui, and P. chinai. Areas of defined environmental variation, geological change, and trans domain fluid fauna, such as the American Transition Zone and the Pacific Domain of Morrone, had the highest Panstrongylus diversity. Pan-biogeographic nodes appear to be areas of the greatest species diversity that act as corridors connecting biotopes and allowing fauna migration. Vicariance events in the geologic history of the continent need to be investigated. The geographical distribution of Panstrongylus overlapped with CD cases and Didelphis marsupialis/Dasypus novemcinctus presence, two important reservoirs in Central and South America. The information derived from the distribution of Panstrongylus provides knowledge for surveillance and vector control programs. It would increase information on the most and less relevant vector species of this zoonotic agent, for monitoring their population behavior.

Introduction: The sensitivity and specificity of diagnostic techniques for Chagas disease depend largely on the antigens and targets used and on the immune response and characteristics of the infection of the population where it is applied, hence the need for evaluation of the diagnostic techniques available in a given area. So, the objective of this work was to evaluate two commercial kits for the immunological and molecular diagnosis of Chagas disease in endemic areas of Venezuela. Methods: The evaluated kits were: Chagas ELISA IgG +IgM (R) and Speed Oligo Chagas (R) (Vircell (R), Granada, Spain). They were evaluated with 129 samples (35 from patients in the acute phase, 33 in the chronic phase, 31 from patients with other diseases, and 30 from healthy individuals). The results were compared with those obtained in the conventional ELISA and PCR-satellite DNA tests for Trypanosoma cruzi. Results: With Chagas ELISA IgG +IgM (R) a sensitivity of 94.1% and specificity of 93.4% were obtained, with Speed Oligo Chagas (R) a sensitivity of 92.6% and specificity of 100% were achieved, values similar to those showed by conventional ELISA and satDNA-PCR. Conclusion: The sensitivity and specificity of the commercial kits evaluated make them suitable for the diagnosis of Chagas disease in endemic areas of Venezuela. (C) 2020 Sociedad Espanola de Enfermedades Infecciosas y Microbiologla Clinica. Published by Elsevier Espana, S.L.U. All rights reserved.