PDF file - 166KB, Supplementary Table 1. Human and murine primer.

PDF file - 6319K, Relationship between size of primary tumor and intravasation. Analyses of lymphatic vessel, cancer stem cell and macrophage density in primary tumors.

PDF file - 207KB, GDF15/MIC-1 is expressed by LNCaP prostate cancer cells and NIH-GDF15 fibroblasts.

PDF file - 1652K, Analysis of associations between STC1 expression and stroma abundance and survival.

PDF file - 117KB, Prostate cancer cells, NIH-GDF15 fibroblasts and CAFs secrete human GDF15/MIC-1.

FIGURES. Fig S1, Bioinformatics reveal potential CXCL14-receptor candidates; Fig. S2, Expression of CXCL14 correlates with EMT in breast cancer patients; Fig. S3, Expression of CXCL14 correlates with EMT in breast, prostate and ovarian cancer patients; Fig. S4, Enhanced expression of CXCL14 and ACKR2 correlates with EMT in ovarian and prostate cancer patients; Fig. S5, CXCL14-fibroblasts stimulate the EMT program in xenograft tumors; Fig. S6, Soluble factors secreted by CXCL14-fibroblasts induce EMT of breast cancer cells in vitro; Fig. S7, CXCL14-fibroblasts enhance invasion of breast cancer cells; Fig. S8, CXCL14-responsive and non-responsive breast cancer cell lines; Fig. S9, CXCL14 signals through a Gαi-coupled receptor; Fig. S10, CXCL14 responses depend on ACKR2; Fig. S11, Downregulation of ACKR2 abolishes CXCL14-induced ERK signaling; Fig. S12, CXCL14 is not a direct ligand of ACKR2; Fig. S13, Correlation of CXCL14 expression levels and EMT does not reflect stroma abundance; Fig. S14, Enhanced expression of CXCL14 and ACKR2 predicts worse outcome in breast cancer patients; Fig. S15, Identification of potential mediators of CXCL14-fibroblast- stimulated breast cancer EMT; Fig. S16, A CXCL14-ACKR2-NOS1 axis in fibroblasts stimulates EMT and invasion of breast cancer cells // TABLES. Table S1, Primers for the amplification of human and murine genes by qRT-PCR; Table S2, Scored levels of E-Cadherin and CytK8/18 in MCF7 breast xenograft tumors, and CytK8/18 in LnCaP prostate xenograft tumors; Table S3, CXCL14 candidate receptor expression in CXCL14-responsive and non-responsive cell lines; Table S4, Multivariable analysis for CXCL14high/ACKR2high subgroup of breast cancer patients from the TCGA gene expression dataset; Table S5, Univariable cox-regression analyses for the CXCL14 (high)/ACKR2(high) subgroup (90-percentile) in publicly available gene expression datasets (TCGA) from 13 different tumor types.

PDF file - 42KB, Nos1 is induced upon oxidative stress in NIH3T3 fibroblasts.

PDF file - 157KB, Fibroblast-derived GDF15/MIC-1 stimulates cytokine production by LNCaP cells.

PDF file - 275KB, GDF15/MIC-1 serum levels correlate with "responder" tumor growth.

PDF file - 6235K, "Route-specific" analysis of metastases.

PDF file - 2355K, Analyses of PDGF β-receptor expression in LIM1215 co-cultured with BJHTERT fibroblasts and PDGFβR ligand production of colon tumors cells. Basal migration levels and migration levels with co-cultured stimulated PDGF fibroblasts of colon cancer cell lines.

PDF file - 46KB, Nos1 knockdown in CXCL14-fibroblasts.

PDF file - 135KB, Recruitment of blood vessels into fibroblast-stimulated prostate and breast tumors.

PDF file - 46KB, NOS1 and CXCL14 mRNA expression correlates in human breast cancer data sets.

PDF file - 8870K, Histopathological analysis of primary tumors.

PDF file - 59KB, NO and glutathione protect CXCL14-fibroblasts.

PDF file - 122KB, Lymphatic vessel recruitment into fibroblast-stimulated prostate and breast tumors.

PDF file - 53KB, Enhanced activation of Hif1α-signaling in CXCL14-fibroblasts.

PDF file - 1263K, PDGF-dependent stimulation by MEFs of colon cancer cell invasion. STC1-dependent stimulation by fibroblasts of colon cancer cell migration.