目的 探讨过继转移日本血吸虫感染鼠DC对IL-13细胞因子调控抑制过敏性哮喘黏液分泌的作用机制.方法 采用MACS磁珠分选方法从日本血吸虫(SJ)感染小鼠及健康对照小鼠脾脏中提取DC,分别过继转移给BALB/c小鼠,并用OVA诱发哮喘.4周后处死小鼠,提取肺组织总RNA并制作病理切片,采取qRT-PCR和PAS染色方法等检测IL-13 mRNA以及蛋白水平.建立DC与CD4+T细胞共培养体系,检测IL-13的表达情况. 结果 肺组织病理学检查显示,与OVA单纯哮喘组比较,过继转移SJ感染DC组(SJ+OVA)过敏性哮喘被抑制.qRT-PCR显示,过继转移SJ感染DC组IL-13 mRNA表达受到抑制.PAS染色显示SJ感染DC组黏液分泌减少,免疫组化检查该组IL-13的表达量降低.DC与CD4+T细胞共培养结果显示SEA处理DC能抑制CD4+T细胞IL-13的表达. 结论 过继转移日本血吸虫感染鼠DC通过下调CD4+T细胞中IL-13细胞因子的释放而抑制过敏性哮喘的黏液分泌.

目的 探讨日本血吸虫(Schistosoma ja ponicum,SJ)感染鼠树突状细胞(Dendritic cell, DC)亚型对过敏性哮喘的抑制作用及其机制. 方法 采用MACS方法从SJ感染鼠脾脏中提取CD8α+ DC (SJCD8α+ DC)和CD8αDC(SJCD8dDC),分别过继转移给BALB/c小鼠,并用OVA诱发哮喘(SJCD8α+组和SJCD8α-组),以正常组(Normal组)和单纯诱发哮喘组(Asthma组)作为对照.取各组小鼠肺组织作石蜡切片,HE染色后观察肺组织病理变化.采用qRT-PCR检测SJCD8α+ DC和SJCD8α DC中IL-12和IL-10 mRNA水平,以正常鼠CD8α+ DC(NCD8α+ DC)和正常鼠CD8α-DC(NCD8α-DC)作为对照.用ELISA方法检测各组小鼠支气管肺泡灌洗液(Bronchial alveolar lavage fluid,BALF)和脾细胞培养上清中IL-10水平. 结果 肺组织病理学检查显示DC过继转移组过敏性哮喘被抑制,其中SJCD8α-组的抑制效果强于SJCD8α+组.SJCD8α-DC IL-10 mRNA表达水平高于SJCD8α+ DC(P＜0.05).并且,SJCD8α-组的BALF和脾细胞培养上清中IL-10蛋白表达水平明显高于SJCD8α+组(P＜0.01,P＜0.01).SJCD8α-DC和SJCD8α+ DC的IL-10 mRNA水平分别为1.73±0.24和1.03±0.03 (GAPDH％).SJCD8α组和SJCD8α+组的BALFIL-10蛋白水平分别为411.21±6.38 pg/ml和202.88±25.30 pg/ml,两组的脾细胞培养上清中IL-10蛋白水平分别为841.53±7.75 pg/ml和254.16±21.46 pg/ml. 结论 过继转移SJCD8α-DC可能通过高表达IL-10抑制OVA诱导的过敏性哮喘.

Objective To study the inhibition of OVA-induced allergic asthma by CD11c+CD8α-dendritic cell(DC) subsets from mice immunized with Schistosoma japonicum egg antigens(SEA).Methods BALB/c mice were injected with 50 μg SEA through the abdominal cavity and foot pad once a week for 4 times in total.Magnetic beads carrying antibodies were used to separate DC subsets into CD11c+CD8α+DCs and CD11c+CD8α-DCs.Eighteen BALB/c mice were randomly divided into four groups: Group A,health control;Group B,asthma alone;Group C,SEA-immune CD11c+CD8α+ DCs;and Group D,SEA-immune CD11c+CD8α-DCs.5×105 CD11c+CD8α+ DCs and CD11c+CD8α-DCs were adoptively transferred to mice in Group C and Group D,respectively,via the caudal vein.One hour later,mice in Groups B,C,and D developed asthma due to OVA at the same time.Four weeks later,all mice were sacrificed and lung tissue was removed to prepare pathology sections;inflammatory changes were observed after HE staining.Results There was no inflammatory reaction in lung tissue from Group A.Severe and extensive inflammation was noted in Group B,which had massive infiltration of inflammatory cells around the bronchi as well as the alveoli.There was marked inflammation in Group C.Compared to Groups B and C,Group D had marked less inflammation,and little infiltration of inflammatory cells was noted.Conclusion CD11c+CD8α-DCs from mice immunized with Schistosoma japonicum SEA may inhibit allergic asthma.