Objective To investigate the role of endothelial bioreacter device in sepsis porcine model.Method Sepsis porcine model was induced gy established endotoxin (LPS,0.25 mg·kg~(-1)) in healthy hybrid swines. The animals were randomly divided(random number) into endothelial bioreactor device group(EBR group) and sham circulation group( Sham group)( n = 6, respectively). After the infusion of endotoxin, extracorporeal circulation was started with the blood flow of 30 mL/min. The blood went through the endothelial bioreactor, then went back to the body via internal jugular vein in the EBR group. The bioreactor with the same size and without endothelial cells(ECs) was used in the sham group. Hemodynamic variables, blood biochemistry, inflammatory markers, Endothelin-1(ET-11) and yon Willebrand Factor(vWF) were examined just before and every hour after the injection. When the survival time of the animals was recorded,the animals were sacrificed to calculate the lung injury score. The time-dependent hemodynamics and cytokine data were compared between groups by repeated measurement ANOVA .Student's t -test was used to analyze the survival time. Results The mean artetial blood pressure (MAP) remarkably decreased in both groups after LPS injection, while the decreasing rate in EBR group was significantly lower than that in control group after 2 hours( P < 0.05). The ET- 1 level in EBR group increased after a slight decrease at the beginning, while that in the sham group went on increasing(P<0.01). The vWF levels increased first, then returned to the baseline in the sixth hour in both groups, while the change in EBR group was significantly less than that in the sham group(P<0.05). The Lung Injury Score in EBR-treated group was significantly lower than that in the sham group(6.1 ± 0.9 vs. 8.2 ± 1. 0, P < 0.05). These physiologic and biochemical alterations were associated with a significant advantage to the survivals in the EBR group when compared with the control sham group(6.7 ± 1.32 vs. 5.2 ± 0.61 h, P < 0.01 ). Conclusions Timely intervention in endotoxin shock with EC therapy by using tissue-engineered bioreactor may improve cardiovascular performance and alter the natural course of this disease process, probably via modulating ioflammation and coagulation cascades.

Introduction: Severe sepsis involves a complex response including the activation of lots of cells, inflammatory mediators, and the hemostatic system. Central to this process is an alteration of endothelial cell function. Therefore, we investigated whether an endothelial bioreactor (EBR) would provide a new therapeutic approach to this clinical disorder. Methods: EBR was constructed using a cartridge which contained with nonwoven polytetrafluoroethylene seeded with porcine iliac artery endothelial cell (PIEC). Pigs were intravenously administered with 0.25 mg/kg lipopolysaccharide and immediately placed in an extracorporeal circuit with EBR or sham controls. Results: Compared with the sham group, EBR therapy resulted in significantly higher mean arterial blood pressure and significantly lower plasma von Willebrand factor, endothelin-1 and scores of lung injury. These alterations were associated with a significant survival advantage in the EBR group. Conclusions: Timely intervention with EC therapy in a tissue-engineered bioreactor may improve cardiovascular performance and alter the natural history of endotoxemia sepsis.

Objective To observe the effects of endothelial cells on haemodynamics,lactic acid,IL-1 and IL-10 in porcine sepsis models. Methods We picked up 15 pigs and divided them into 3 groups (5 for each group) randomly:control group,glucocorticosteroid (GC) group and glucocorticosteroid plus endothelial cells(GC+EC) group. Lipopolysaccharide (LPS) was given by intravenous injection in all three groups and then we gave methylprednisolone(MP) 250mg to the GC group and MP 250mg plus 108 endothelial cells via arteria to the GC+EC group. The changes of haemodyanmic,biochemical and inflammatory parameters were recorded. Results The elevation of mean arterial pressure P in GC and GC+EC group were higher than that in the control group(GC:P=0.004;GC+EC:P=0.000). The elevation of pulmonary vascular resistance in GC+EC group was lower than that in the control group(P=0.035),while the elevation in GC group had no significant difference with the control group (P=0.346). WBC recovered sooner in GC group than in control group (P=0.000) or GC group (P=0.000). The elevation of AST in GC group and GC+EC group were lower than that in the control group(GC:P=0.008 GC+EC:P=0.005),but no difference was observed between GC and GC+EC group(P=0.858). IL-10 in GC+EC group was lower than that in the control group(P=0.021) while no significant difference was observed between GC and control group (P=0.158). Conclusions The combination of glucocorticosteroid and endothelial cells improved haemodyanmic indexes,delayed organ failures and modulated inflammatory level. It signifies the rationality of the construction of endothelial cell assist device.